2001
DOI: 10.1073/pnas.201182798
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Abstract: Open reading frame expressed sequences tags (ORESTES) differ from conventional ESTs by providing sequence data from the central protein coding portion of transcripts. We generated a total of 696,745 ORESTES sequences from 24 human tissues and used a subset of the data that correspond to a set of 15,095 full-length mRNAs as a means of assessing the efficiency of the strategy and its potential contribution to the definition of the human transcriptome. We estimate that ORESTES sampled over 80% of all highly and m… Show more

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Cited by 118 publications
(83 citation statements)
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“…Therefore, we constructed a cDNA microarray that is enriched with a collection of partial transcripts that map to intronic segments of known genes. We used a bioinformatics approach to select these from nearly one million ESTs that were generated by the Human Cancer Genome Project with the ORESTES technique (DiasNeto et al, 2000) from 24 distinct types of cancer and normal adjacent tissues, having sampled over 60% of the human genes (Camargo et al, 2001;Brentani et al, 2003). The resulting intron array was used to measure the expression profile of 27 prostate tumor samples with different degrees of differentiation (Gleason score, GS 5 to 10).…”
Section: Introductionmentioning
confidence: 99%
“…Therefore, we constructed a cDNA microarray that is enriched with a collection of partial transcripts that map to intronic segments of known genes. We used a bioinformatics approach to select these from nearly one million ESTs that were generated by the Human Cancer Genome Project with the ORESTES technique (DiasNeto et al, 2000) from 24 distinct types of cancer and normal adjacent tissues, having sampled over 60% of the human genes (Camargo et al, 2001;Brentani et al, 2003). The resulting intron array was used to measure the expression profile of 27 prostate tumor samples with different degrees of differentiation (Gleason score, GS 5 to 10).…”
Section: Introductionmentioning
confidence: 99%
“…Attempts to catalogue the mammalian transcriptome have been based upon assembly of sequences from large-scale expressed-sequence-tag (EST) sequencing projects 9 . More recently, serial analysis of gene expression 10 and large-scale sequencing of open reading frame (ORF) sequence tags 11 have contributed to the definition of the transcriptome and have improved genome annotation. Each has the limitation that no physical cDNA clones are fully sequenced.…”
mentioning
confidence: 99%
“…The limitations of a priori genome annotation dictate that the transcriptome needs to be identified experimentally via cDNA cloning and sequencing. Although expressed sequence tags (ESTs) (Adams et al 1991(Adams et al , 1995Hillier et al 1996;Marra et al 1999;Kargul et al 2001) and ORESTES (Camargo et al 2001) have been extremely valuable for new gene discovery, these approaches have not allowed highthroughput recovering of full-length cDNA clones nor definition of protein sequence derived from actual cDNA clones. To overcome such problems, we undertook from the year 1995, a strategic project aimed at the comprehensive collection of at least one full-length cDNA derived from each mouse gene, a strategy that is recently becoming useful in similar projects to collect full-length gene collections (Stapleton et al 2002;Strausberg et al 2002).…”
mentioning
confidence: 99%