The concentration of ionized magnesium in serum during the periparturient period of non-paretic dairy cows

Riond, J.‐L.; Kocabağlı, Neşe; Spichiger, Ursula E.; Wanner, M.

doi:10.1007/bf01839298

Cited by 27 publications

(24 citation statements)

References 25 publications

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“…Magnesium has no effect on hemostasis when serum levels are less than 3 mM but the coagulation time of whole blood is prolonged when Mg concentration are greater than 7 mM [12]. The total Mg level found in this study for bovine serum was 0.93 ± 0.18 mM/l which is similar to other reports [4,15,18]. However, the Mg content in FF (S) showed a significant high level compared with the value in plasma and the Mg level decreased with follicle development with approximately sera being reached in the largest follicles.…”

Section: Discussionsupporting

confidence: 81%

“…The Gladependent Ca binding is a prerequisite for the interaction between these coagulation factor proteins and biological membranes [23]. In this study, total serum Ca concentration were similar to those closely previously reported [4,16,18,19,22] as were the ionized Ca concentration [11,19]. The ionized Ca concentration in FF was found relatively constant at all stages of follicles and approximated the bovine serum level.…”

Section: Discussionsupporting

confidence: 73%

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Magnesium as a Regulator of Thrombin Formation in Bovine Ovarian Follicular Fluid.

Yamada

Hirakushi

Inoue

et al. 1998

The Journal of Veterinary Medical Science

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ABSTRACT. In the ovarian follicular fluid (FF) of Holstein cows, calcium (Ca) and magnesium (Mg) levels and their roles on thrombin generation were examined and compared with the blood samples. Total Ca levels in FF increased while the total Mg levels decreased with follicular development from preantral to preovulatory stage of follicles. These changes resulted in Ca values being significantly (p<0.05) higher in FF from the most developed follicles and the Mg values being significantly higher (p<0.05) in the least developed follicles. To determine whether the high level of Mg might function to regulate thrombin generation in FF as occurs in plasma, the influence of Mg supplementation of FF from various types of follicles was examined. In FF from small size follicles, Mg accelerated the prothrombin time, an estimation of the overall rate of thrombin production, although a similar effect was not observed in FF from medium and large size follicles. The addition of Mg to FF from all sizes of follicles resulted an inhibition in factor X activation. Since activation of factor X is a precursor step for thrombin formation it is concluded that Mg can function as a slow accelerator of thrombin generation in FF from follicles at the antral stage of development. It is likely to have a more important role in regulating the rate of thrombin generation as the follicle develops. -KEY WORDS: bovine, calcium, follicular fluid, magnesium, thrombin generation.J. Vet. Med. Sci. 60 (7): [837][838][839][840][841][842] 1998 was any connection between the levels of this hormone and Ca and Mg level [1]. Because of the potential role of thrombin as a mitogen in follicular development [7], a second objective of the study was to investigate whether Ca and/or Mg might have a role in thrombin generation during follicular development.To assess the involvement of Ca and Mg level or their concentration ratio to thrombin generation in ovarian follicular fluid, three types of assays were performed: 1) thrombin time assay to determine possible direct effects of Ca and Mg on thrombin activity towards the fibrinogen substrate present in follicular fluid, 2) prothrombin time, an overall measurement of thrombin generation in follicular fluid and 3) a specific factor X activity assay since FXa is key activating enzyme in thrombin generation. The results of assays in FF were compared with those of bovine plasma. MATERIALS AND METHODSBlood and follicular fluid samples: Whole blood and ovaries were obtained at a local abattoir from healthy Holstein cows. The blood was collected directly from the carotid artery without anticoagulant for serum preparation and with 3.8% citrate sodium solution for thrombin generation assays or 10 g/l heparin sodium (Daiichi Pure Chem. Co., Tokyo, Japan) solution for mineral assays to obtain plasma. Serum was prepared by incubating the whole blood clot at 4°C for 24 hr before aspiration of the supernatant. Plasma was prepared by centrifuging anticoagulated blood at 3,000 × g, for 15 min at 4°C. Bovine ovaries were kept at 3...

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Section: Discussionsupporting

confidence: 81%

Section: Discussionsupporting

confidence: 73%

Magnesium as a Regulator of Thrombin Formation in Bovine Ovarian Follicular Fluid.

Yamada

Hirakushi

Inoue

et al. 1998

The Journal of Veterinary Medical Science

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“…The measured strong ion difference obtained from 7 strong ions34 (SID 7 , mEq/L) was calculated by the measured value for [Ca 2+ ] determined by ion‐selective potentiometry and assigning a charge of +1.38 to magnesium assuming 69% dissociation35 and −1 to D‐lactate and L‐lactate assuming 100% dissociation such that:…”

Section: Methodsmentioning

confidence: 99%

Effect of Intravenous Small‐Volume Hypertonic Sodium Bicarbonate, Sodium Chloride, and Glucose Solutions in Decreasing Plasma Potassium Concentration in Hyperkalemic Neonatal Calves with Diarrhea

Trefz

Constable

Lorenz

2017

Veterinary Internal Medicne

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BackgroundHyperkalemia is a frequently observed electrolyte imbalance in dehydrated neonatal diarrheic calves that can result in skeletal muscle weakness and life‐threatening cardiac conduction abnormalities and arrhythmias.HypothesisIntravenous administration of a small‐volume hypertonic NaHCO3 solution is clinically more effective in decreasing the plasma potassium concentration (cK) in hyperkalemic diarrheic calves than hypertonic NaCl or glucose solutions.AnimalsTwenty‐two neonatal diarrheic calves with cK >5.8 mmol/L.MethodsProspective randomized clinical trial. Calves randomly received either 8.4% NaHCO3 (6.4 mL/kg BW; n = 7), 7.5% NaCl (5 mL/kg BW; n = 8), or 46.2% glucose (5 mL/kg BW; n = 7) IV over 5 minutes and were subsequently allowed to suckle 2 L of an electrolyte solution. Infusions with NaHCO3 and NaCl provided an identical sodium load of 6.4 mmol/kg BW.ResultsHypertonic NaHCO3 infusions produced an immediate and sustained decrease in plasma cK. Hypertonic glucose infusions resulted in marked hyperglycemia and hyperinsulinemia, but cK remained unchanged for 20 minutes. Between 30 and 120 minutes after initiation of treatment, the most marked decrements in cK from baseline occurred in group NaHCO3, which were significantly (P < .05) larger during this period of time than in calves in group NaCl, but not group glucose. After 120 minutes, the mean decrease in cK from baseline was −26 ± 10%, −9 ± 8%, and −22 ± 6% in groups NaHCO3, NaCl, and glucose, respectively.Conclusions/Clinical ImportanceSmall‐volume hypertonic NaHCO3 infusions appear to have clinical advantages for the rapid resuscitation of hyperkalemic diarrheic calves, compared to hypertonic NaCl or glucose solutions.

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“…The chemical gradient from 2-4 mM in the lumen, of which 49-100 % is ultrafilterable (Storry, 1961), to 0.8-1*2 mM in blood serum (Moodie, 1975), of which about 70°% is ionized (Riond, Kocabagli, Spichiger & Wanner, 1995), is opposed by a transruminal potential difference of about 30 mV (blood side positive, Dobson & Phillipson, 1958) that overcomes the chemical driving force. Magnesium net absorption across the rumen epithelium is therefore mainly driven by active transport mechanisms (Martens, Harmeyer & Breves, 1976).…”

Section: Introductionmentioning

confidence: 99%

Effects of short chain fatty acids and carbon dioxide on magnesium transport across sheep rumen epithelium

Leonhard-Marek

Gäbel²,

Martens³

1998

Experimental Physiology

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The concentration of ionized magnesium in serum during the periparturient period of non-paretic dairy cows

Cited by 27 publications

References 25 publications

Magnesium as a Regulator of Thrombin Formation in Bovine Ovarian Follicular Fluid.

Magnesium as a Regulator of Thrombin Formation in Bovine Ovarian Follicular Fluid.

Effect of Intravenous Small‐Volume Hypertonic Sodium Bicarbonate, Sodium Chloride, and Glucose Solutions in Decreasing Plasma Potassium Concentration in Hyperkalemic Neonatal Calves with Diarrhea

Effects of short chain fatty acids and carbon dioxide on magnesium transport across sheep rumen epithelium

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