The complexity of Trypanosoma cruzi populations revealed by schizodeme analysis

Morel, Carlos Médicis; Deane, Maria P.; Gonçalves, A. Pedro

doi:10.1016/0169-4758(86)90038-4

Cited by 36 publications

(24 citation statements)

References 32 publications

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“…A high degree of variability was corroborated and extended when more loci where analyzed and after the application of a plethora of distinct molecular methods (Morel et al 1986, Tibayrenc & Ayala 1988, Macedo et al 1992, Henriksson et al 1996. Nevertheless, this diversity could not be consistently correlated with a particular clinical presentation, epidemiological circumstances of transmission or geographical origin.…”

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confidence: 77%

Genetic diversity of Colombian sylvatic Trypanosoma cruzi isolates revealed by the ribosomal DNA

Cuervo

Cupolillo

Segura

et al. 2002

Mem. Inst. Oswaldo Cruz

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confidence: 77%

Genetic diversity of Colombian sylvatic Trypanosoma cruzi isolates revealed by the ribosomal DNA

Cuervo

Cupolillo

Segura

et al. 2002

Mem. Inst. Oswaldo Cruz

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“…Similarly the "Y" strain derived from certain laboratories showed selected subpopulations in monoclonal material and sometimes laboratory mixups, according to Morel et al 22 Therefore the poor contribution of these stocks to the vector-parasite interaction, as shown in Fig. 3, could be due to the heterogeneity of the parasite stocks.…”

Section: Discussionmentioning

confidence: 99%

Studies in search of a suitable experimental insect model for xenodiagnosis of hosts with Chagas' disease: 4 - The reflection of parasite stock in the responsiveness of different vector species to chronic infection with different Trypanosoma cruzi stocks

Perlowagora-Szumlewicz¹,

Müller²,

Moreira³

1990

Rev. Saúde Pública

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Previous studies (1982,1987) have emphasized the superiority of sylvatic vector species over domestic species as xenodiagnostic agents in testing hosts with acute or chronic infections by T. cruzi "Y" stock. The present study, which is unique in that it contains data on both infectivity rates produced by the same stock in 11 different vector species and also the reaction of the same vector species to seven different parasite stocks, establishes the general validity of linking efficiency of xenodiagnosis to the biotope of its agent. For example, infectivity rates produced by "São Felipe" stock varied from 82.5% to 98.3% in sylvatic vectors but decreased to 42.5% to 71.3% in domestic species. "Colombiana" stock produced in the same sylvatic vectors infectivity rates ranging from 12.5% to 45%. These shrank to 5%-22.5% in domestic bugs. The functional role of the biotope in the vector-parasite interaction has not been eluddated. But since this phenomenon has been observed to be stable and easy to reproduce, it leads us to believe that the results obtained are valid. Data presented also provide increasing evidence that the infectivity rates exhibited by bugs from xenodiagnosis in chronic hosts, are parasite stock specific. For example, infectivity rates produced by "Berenice", "Y", "FL" and "CL" varied in R. neglectus from 26.3% to 75%; in P. megistus from 56.3% to 83.8%; in T. sordida from 28.8% to 58.8% in T. pseudomaculata from 41.3% to 66.3% and in T. rubrovaria from 48.8% to 85%. Data from xenodiagnosis in the same hosts, carrying acute infections by the same parasite stocks, gave the five sylvatic vectors a positive rating of approximately 100%, thus suggesting that the heavy loads of parasites circulating in the acute hosts obscured the characteristic interspecific differences for the parasite stock. Nonetheless these latter were revealed in the same hosts with chronic infections stimulated by very low numbers of the same parasite stocks. Certain observations here described lead us to speculate as to the possibility of further results from other parasite stocks, allowing the association of the infectivity rates produced in bugs by different parasite stocks with the isoenzymic patterns revealed by these stocks.

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“…Although this finding has not proved particularly useful in the choice of xenodiagnostic agent candidates, it is of major importance to investigators involved in classification of T. cruzi strains on the basis of isoenzyme profiles reported by Miles & Cibulskis 10 , in the analysis of infection of bugs with single-cell-isolate clones of T. cruzi, described by Garcia & Dvorak 7 and also for the characterization of parasites on the genotype level at least until new techniques that would not require parasite population amplification are developed, as stated in 1986 by Morel et al 12 . Consequently this insect will have more enthusiastic backing among investigators mentioned above but, there will be some scepticism for using it in field surveys.…”

Section: And Recomendationsmentioning

confidence: 99%

Studies in search of a suitable experimental insect model for xenodiagnosis of hosts with Chagas' disease: 3 - On the interaction of vector species and parasite strain in the reaction of bugs to infection by Trypanosoma cruzi

Perlowagora-Szumlewicz¹,

Müller²,

Moreira³

1988

Rev. Saúde Pública

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ABSTRACT:The reaction of nine vector species of Chagas' disease to infection by seven different Trypanosoma cruzi strains; Berenice, Y, FL, CL, S. Felipe, Colombiana and Gávea, are examined and compared. On the basis of the insects' ability to establish and maintain the infection, vector species could be divided into two distinct groups which differ in their reaction to an acute infection by T. cruzi. While the proportion of positive bugs was found to be low in Triatoma infestans and Triatoma dimidiata it was high, ranging from 96.9% to 100% in the group of wild (Rhodnius neglectus, Triatoma rubrovaria)and essentially sylvatic vectors in process of adaptation to human dwellings, maintained under control following successful insecticidal elimination of Triatoma infestans (Panstrongylus megistus, Triatoma sordida and Triatoma pseudomaculata). An intermediate position is held by Triatoma brasiliensis and Rhodnius prolixus. This latter has been found to interchange between domestic and sylvatic environments. The most important finding is the strikingly good reaction between each species of the sylvatic bugs and practically all T. cruzi strains herein studied, thus indicating that the factors responsible for the excellent reaction of P.megistus to infection by Y strain, as previously reported also come into operation in the reaction of the same vector species to acute infections by five of the remaining T.cruzi strains. Comparison or data reported by other investigators with those herein described form the basis of the discussion of Dipetalogaster maximus as regards its superiority as a xenodiagnostic agent.

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The complexity of Trypanosoma cruzi populations revealed by schizodeme analysis

Cited by 36 publications

References 32 publications

Genetic diversity of Colombian sylvatic Trypanosoma cruzi isolates revealed by the ribosomal DNA

Genetic diversity of Colombian sylvatic Trypanosoma cruzi isolates revealed by the ribosomal DNA

Studies in search of a suitable experimental insect model for xenodiagnosis of hosts with Chagas' disease: 4 - The reflection of parasite stock in the responsiveness of different vector species to chronic infection with different Trypanosoma cruzi stocks

Studies in search of a suitable experimental insect model for xenodiagnosis of hosts with Chagas' disease: 3 - On the interaction of vector species and parasite strain in the reaction of bugs to infection by Trypanosoma cruzi

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