The complete mitochondrial genome sequence of the Daweishan Mini chicken

Abstract

Daweishan Mini chicken is a valuable chicken breed in China. In this study, the complete mitochondrial genome sequence of Daweishan Mini chicken using PCR amplification, sequencing and assembling has been obtained for the first time. The total length of the mitochondrial genome was 16,785 bp, with the base composition of 30.26% A, 23.73% T, 32.51% C, 13.51% G. It contained 37 genes (2 ribosomal RNA genes, 13 protein-coding genes, 22 transfer RNA genes) and a major non-coding control region (D-loop region). The protein start codons are ATG, except for COX1 that begins with GTG. The complete mitochondrial genome sequence of Daweishan Mini chicken provides an important data set for further investigation on the phylogenetic relationships within Gallus gallus.

Complete mitochondrial DNA and phylogenetic study of qionglai native black chicken

“…The gene arrangement and composition were similar to those of other avian species [10,11]. The overall nucleotide composition of A, T, C and G were found to be 30.28%, 23.74%, 32.50% and 13.48%, respectively, and in the order C>A>T>G. Most of the genes are encoded on the H-strand and they are similar in structure to the typical mitochondrial genome of vertebrates [12].…”

Complete mitochondrial genome sequence and gene organization of Chinese indigenous chickens with phylogenetic considerations

“…The sequence lengths differed slightly, mainly due to changes in the CR. The overall A + T contents of the Rugao yellow and Jinhu Black-bone chicken mtDNA genomes were 54.04 and 53.96%, which were similar to those of other chicken breeds, such as Daweishan Mini chickens (Yan et al, 2016), Gallus domesticus Brisson , and Huanglang chickens (Yu et al, 2016). The Rugao yellow and Jinhu Black-bone chickens possessed 13 gene overlaps and five intergenic spacers, which differs from other chicken breeds (Liu et al, 2016a,b).…”

“…Protein-coding genes were determined by sequence comparisons using specialized BLAST at the NCBI database site. Next, these 13 genes were checked with the known complete mtDNA sequence of closely related species, including Daweishan Mini chickens (Yan et al, 2016), Huang Lang chickens (Yu et al, 2016), Taoyuan chickens (Liu et al, 2016b, and Xuefeng black-boned chickens (Liu, et al, 2016a). Twenty-one tRNA genes and their anticodons were annotated online using tRNAscan-SE Search Server v.1.21 (http://lowelab.ucsc.edu/tRNAscan-SE/) (Lowe and Eddy, 1997).…”

Complete mitochondrial genome of the Partridge Shank chicken (Galliformes: Phasianidae)

“…It consisted of 37 mitochondrial genes, including 13 protein-coding genes (PCGs), 22 transfer RNAs, 2 rRNA (12S rRNA and 16S rRNA) and a non-coding control region (D-loop region). The arrangement of all genes and transcriptional direction were identical with those of typical Gallus (Desjardins and Morais 1990;Liu et al 2016;Yan et al 2016). The mitochondrial genome was very closely arranged with gene overlaps.…”