The comparative performance of the single intradermal comparative tuberculin test in Irish cattle, using tuberculin PPD combinations from different manufacturers

Good, Margaret; Clegg, Tracy A.; Murphy, Fionna; More, Simon J.

doi:10.1016/j.vetmic.2011.02.028

Cited by 23 publications

(20 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although this is until now only stated for the skin test and in context of false negative test results [69]. The specificity of the Bovigam® assay tests varies also with the concentration and potency of PPDs [70], which can differ remarkably [71, 72]. These influences might explain to some extend the estimated low specificities of the Bovigam® assay.…”

Section: Discussionmentioning

confidence: 99%

Evaluating diagnostic tests for bovine tuberculosis in the southern part of Germany: A latent class analysis

et al. 2017

View full text Add to dashboard Cite

Germany has been officially free of bovine tuberculosis since 1996. However, in the last years there has been an increase of bovine tuberculosis cases, particularly in the southern part of Germany, in the Allgäu region. As a consequence a one-time tuberculosis surveillance program was revisited with different premortal and postmortal tests. The aim of this paper was to estimate diagnostic sensitivities and specificities of the different tests used within this surveillance program. In the absence of a perfect test with 100% sensitivity and 100% specificity, thus in the absence of a gold standard, a Bayesian latent class approach with two different datasets was performed. The first dataset included 389 animals, tested with single intra-dermal comparative cervical tuberculin (SICCT) test, PCR and pathology; the second dataset contained 175 animals, tested with single intra-dermal cervical tuberculin (SICT) test, Bovigam® assay, pathology and culture. Two-way conditional dependencies were considered within the models. Additionally, inter-laboratory agreement (five officially approved laboratories) of the Bovigam® assay was assessed with Cohen's kappa test (21 blood samples). The results are given in posterior means and 95% credibility intervals. The specificities of the SICT test, SICCT test, PCR and pathology ranged between 75.8% [68.8–82.2%] and 99.0% [96.8–100%]. The Bovigam® assay stood out with a very low specificity (6.9% [3.6–11.1%]), though it had the highest sensitivity (95.7% [91.3–99.2%]). The sensitivities of the SICCT test, PCR, SICT test, pathology and culture varied from 57.8% [48.0–67.6%] to 88.9% [65.5–99.7%]. The prevalences were 19.8% [14.6–26.5%] (three-test dataset) and 7.7% [4.2–12.3%] (four-test dataset). Among all pairwise comparisons the highest agreement was 0.62 [0.15–1]). In conclusion, the specificity of the Bovigam® assay and the inter-laboratory agreement were lower than expected.

show abstract

Section: Discussionmentioning

confidence: 99%

Evaluating diagnostic tests for bovine tuberculosis in the southern part of Germany: A latent class analysis

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Variations in PPD production methods may lead to different antigenic profiles and therefore to qualitative differences between PPDs (Seibert and DuFour, 1940; Landi and McClure, 1969; Haagsma et al., 1982; Tameni et al., 1998). Moreover, the in vivo potency tests are inherently variable and of questionable precision, demonstrated by studies with repeated batch testing (Good et al., 2008). Importantly, a recent comparison of commercially available tuberculins has shown that if applied in a dose of 1 mg/ml, the majority of these tuberculins would not meet the required minimum dose of 2000 International Units (Bakker et al., 2009).…”

Section: Current Testing Strategiesmentioning

confidence: 99%

Bovine Tuberculosis: A Review of Current and Emerging Diagnostic Techniques in View of their Relevance for Disease Control and Eradication

Schiller

Oesch²,

Vordermeier

et al. 2010

Transboundary and Emerging Diseases

209

274

View full text Add to dashboard Cite

Summary Existing strategies for long‐term bovine tuberculosis (bTB) control/eradication campaigns are being reconsidered in many countries because of the development of new testing technologies, increased global trade, continued struggle with wildlife reservoirs of bTB, redistribution of international trading partners/agreements, and emerging financial and animal welfare constraints on herd depopulation. Changes under consideration or newly implemented include additional control measures to limit risks with imported animals, enhanced programs to mitigate wildlife reservoir risks, re‐evaluation of options to manage bTB‐affected herds/regions, modernization of regulatory framework(s) to re‐focus control efforts, and consideration of emerging testing technologies (i.e. improved or new tests) for use in bTB control/eradication programs. Traditional slaughter surveillance and test/removal strategies will likely be augmented by incorporation of new technologies and more targeted control efforts. The present review provides an overview of current and emerging bTB testing strategies/tools and a vision for incorporation of emerging technologies into the current control/eradication programs.

show abstract

“…The other method is through slaughter surveillance that is entirely based on meat inspection at the slaughterhouse. The SCT has practical drawbacks such as subjectivity in result interpretation (the interpretation is based on observation and the recorded increases in skin‐fold thickness) and lack of specificity (false‐positive reactions caused by sensitization by other mycobacteria and by local inflammation) (Rua‐Domenech et al., ; Good et al., ; Fernández et al., ; Humblet et al., ; Bezos et al., ). The single comparative cervical tuberculin test (SCIT) is not used by the GOVs due to the lack of financial support.…”

Section: Introductionmentioning

confidence: 99%

Prevalence of Bovine Tuberculosis in Egyptian Cattle and the Standardization of the Interferon-gamma Assay as an Ancillary Test

Abdellrazeq

Elnaggar

Osman

et al. 2014

Transbound Emerg Dis

View full text Add to dashboard Cite

Bovine tuberculosis (bTB) caused primarily by Mycobacterium bovis continues to cause significant losses in the cattle industry and is a major public health problem. Despite its worldwide application, the IFN-γ assay has not been applied in Egypt. The aim of this study was to determine the appropriate cut-off value of IFN-γ assay to complement the skin test screening in Egypt. The relative sensitivity (Ser ) of PPD and antigen cocktail-based IFN-γ assays (IFN-γ-BA and IFN-γ-EC) was analysed retrospectively, relative to bTB confirmatory tests (culture and PCR), using single cervical tuberculin (SCT) test reactors during 2011-2013. The absolute specificity (Sp) was studied using blood samples collected from cattle from one bTB-free herd. Analysis of the bTB database-generated sheets indicates the infection rate had decreased from 2009 to 2012 and then increased in 2013. The disease is concentrated in the Egyptian Nile Delta and Valley relative to elsewhere in the country. The cut-offs for IFN-γ-EC assay could be optimized to provide higher sensitivity, comparable to cut-offs for IFN-γ-BA assay. Data analysis suggests (PPDbOD > 0.1, PPDbOD - NILOD > 0.05 and PPDbOD > PPDaOD ) and (ECOD - NILOD ≥ 0.1) cut-off strategies to get optimal IFN-γ-BA and IFN-γ-EC assays results respectively. To our knowledge, this is the first report describing the prevalence of bTB in cattle in Egypt and pointing out the appropriate cut-off criteria to optimize IFN-γ assay as a routine ancillary test for diagnosis of bTB in Egypt.

show abstract

The comparative performance of the single intradermal comparative tuberculin test in Irish cattle, using tuberculin PPD combinations from different manufacturers

Abstract: 13Ireland currently obtains its avian and bovine tuberculin purified protein derivatives (PPDs) from a 14 single source. Because problems of supply or quality cannot be discounted, it is prudent that Ireland

Cited by 23 publications

References 11 publications

Evaluating diagnostic tests for bovine tuberculosis in the southern part of Germany: A latent class analysis

Evaluating diagnostic tests for bovine tuberculosis in the southern part of Germany: A latent class analysis

Bovine Tuberculosis: A Review of Current and Emerging Diagnostic Techniques in View of their Relevance for Disease Control and Eradication

Prevalence of Bovine Tuberculosis in Egyptian Cattle and the Standardization of the Interferon-gamma Assay as an Ancillary Test

Contact Info

Product

Resources

About