The comparative effects of nano and bulk size particles of CuO and ZnO on glycyrrhizin and phenolic compounds contents in Glycyrrhiza glabra L. seedlings

Oloumi, Hakimeh; Soltaninejad, Razieh; Baghizadeh, Amin

doi:10.1007/s40502-015-0143-x

Cited by 74 publications

(35 citation statements)

References 22 publications

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“…In our study, nano-TiO 2 treated plants at certain concentrations caused higher phenolic and flavonoid contents than control untreated plants. Our results are in confirmation of the findings of Oloumi et al (2015), who reported that nano-sized CuO and ZnO particles had positive effects on secondary metabolite contents, including phenolic compounds and glycyrrhizin content compared to their bulk size in Glycyrrhiza glabra seedlings. Other studies also showed an increase in secondary metabolites production including total phenolic (Diaz et al 2001) and flavonoid (Cristina and Constantin 2011) following abiotic elicitor application.…”

Section: Extract Yield and Total Phenolic And Flavonoid Contentssupporting

confidence: 92%

Major essential oil constituents, total phenolics and flavonoids content and antioxidant activity of Salvia officinalis plant in response to nano-titanium dioxide

Ghorbanpour

2015

Ind J Plant Physiol.

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Medicinal plants produce a wide range of secondary metabolites to increase their performance upon exposure to various kinds of elicitors. The present study was undertaken to evaluate the impacts of various concentrations of nano-titanium dioxide (TiO 2 ) particles, viz., 0, 10, 50, 100, 200 and 1000 mg l -1 on growth, biosynthesis of secondary metabolites such as essential oils, total phenolics, flavonoids and antioxidant efficacy of the extracts obtained from the treated Salvia officinalis plants, assayed using DPPH free radical scavenging and b-carotene/linoleic acid bleaching test systems. The results showed that the highest and the lowest dry weights of root and shoot tissues were obtained from plants exposed to 100 and 1000 mg l -1 TiO 2 , respectively. The highest total phenolic (35.2 mg GAE g -1 dry wt.) and flavonoid (21.9 mg CE g -1 dry wt.) contents were obtained in plants exposed to 200 and 100 mg l -1 nano-TiO 2 , respectively. Gas chromatography and mass spectrometry analysis showed that the major components of essential oils were monoterpenes, including Camphene, p-Cymene, 1,8-Cineol, Cis-Thujene and Camphor. The highest value of Cis-Thujene (34.5 %) and 1,8-Cineol (21.2 %) were achieved in extracts obtained from plants exposed to 200 mg l -1 nano-TiO 2 . However, the maximal content of Camphene (12.1 %) was obtained from plants exposed to 1000 mg l -1 nano-TiO 2 treatment. Although the extract from all treated plants improved antioxidant activity, it was highest for the extract obtained from plants treated with nano-TiO 2 at 200 mg l -1 with an IC 50 value of 2.44 lg g -1 dry wt. as compared to that of control plants (6.42 lg g -1 dry wt.) and butylated hydroxytoluene as positive probe (3.68 lg g -1 dry wt.). It was suggested for the first time that nano-TiO 2 may act as an elicitor to improve secondary metabolism in S. officinalis plants for biosynthesis of natural antioxidants.

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Section: Extract Yield and Total Phenolic And Flavonoid Contentssupporting

confidence: 92%

Major essential oil constituents, total phenolics and flavonoids content and antioxidant activity of Salvia officinalis plant in response to nano-titanium dioxide

Ghorbanpour

2015

Ind J Plant Physiol.

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“…The TPC in extracts of H. reticulatus hairy roots increased after elicitation with the various ZnO concentrations and exposure times in comparison with non-elicited hairy roots. The few published studies on plant metabolite production in response to nanoparticle elicitation diverge in the effects of submicron-size particles on natural metabolites in cell and organ cultures [7]. The few published studies on plant metabolite production in response to nanoparticle elicitation diverge in the effects of submicron-size particles on natural metabolites in cell and organ cultures [7].…”

Section: Discussionmentioning

confidence: 99%

“…For instance, the artemisinin content was increased in a hairy root culture of Artemisia annua upon treatment with silver nanoparticles [6]. In Glycyrrhiza glabra, nanoparticles of zinc oxide and copper oxide increased the content of glycyrrhizin, total phenols, flavonoids, anthocyanin and proline in comparison to the control [7]. In H. reticulatus, the highest hyoscyamine and scopolamine production (an increase of about five-fold compared to the control) was achieved with 900 and 450 mg/L FeNPs at 24 and 48 h of exposure time, respectively [8].…”

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confidence: 99%

Influence of nano‐zinc oxide on tropane alkaloid production, h6h gene transcription and antioxidant enzyme activity in Hyoscyamus reticulatus L. hairy roots

Asl

Hosseini

Sharafi

et al. 2018

Engineering in Life Sciences

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The use of nanotechnology and biotechnology to improve the production of plant bioactive compounds is growing. Hyoscyamus reticulatus L. is a major source of tropane alkaloids with a wide therapeutic use, including treatment of Parkinson's disease and to calm schizoid patients. In the present study, hairy roots were obtained from two‐week‐old cotyledon explants of H. reticulatus L. using the A7 strain of Agrobacterium rhizogenes. The effects of different concentrations of the signaling molecule nano‐zinc oxide (ZnO) (0, 50, 100 and 200 mg/L), with three exposure times (24, 48 and 72 h), on the growth rate, antioxidant enzyme activity, total phenol contents (TPC), tropane alkaloid contents and hyoscyamine‐6‐beta‐hydroxylase (h6h) gene expression levels were investigated. Growth curve analysis revealed a decrease in fresh and dry weight of ZnO‐treated hairy roots compared to the control. ANOVA results showed that the antioxidant activity of the enzymes catalase, guaiacol peroxidase and ascorbate peroxidase was significantly higher in the ZnO‐treated hairy roots than in the control, as was the TPC. The highest levels of hyoscyamine (37%) and scopolamine (37.63%) were obtained in hairy roots treated with 100 mg/L of ZnO after 48 and 72 h, respectively. Semi‐quantitative RT‐PCR analysis revealed the highest h6h gene expression was in hairy roots treated with 100 mg/L of ZnO after 24 h. It can be concluded that ZnO is as an effective elicitor of tropane alkaloids such as hyoscyamine and scopolamine due to its enhancing effect on expression levels of the biosynthetic h6h gene.

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“…In Silybum marianum hairy root cultures, the greatest accumulation of silymarin was in roots treated with 2 mM silver nanoparticles, which was twofold higher when compared with the non‐elicited roots . The CuO and ZnO nanoparticles in Glycyrrhiza glabra hairy root cultures achieved the highest glycyrrhizin content . The amount of saponin content in Calendula officinalis increased compared to the control roots after treatment with 0.4 mM nanosilver .…”

Section: Introductionmentioning

confidence: 94%

“…30 The CuO and ZnO nanoparticles in Glycyrrhiza glabra hairy root cultures achieved the highest glycyrrhizin content. 31 The amount of saponin content in Calendula officinalis increased compared to the control roots after treatment with 0.4 mM nanosilver. 32 Due to the importance of the pharmaceutical and industrial applications of RA, its production has been studied in various plant species (Orthosiphon aristatus, Salvia miltiorrhiza, and Melissa officinalis) influenced by biological and non-biological elicitors.…”

Section: Introductionmentioning

confidence: 94%

Iron oxide nanoparticles: a novel elicitor to enhance anticancer flavonoid production and gene expression in Dracocephalum kotschyi hairy‐root cultures

et al. 2019

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BACKGROUND Dracocephalum kotschyi Boiss. is a valuable source of rosmarinic acid (RA) and methoxylated hydroxyflavones (such as xanthomicrol and cirsimaritin) with antioxidative and antiplatelet effects and with antiproliferative potential against various cancer cells. The extensive application of nanotechnology in hairy root cultures is a new sustainable production platform for producing these active constituents. In the present study, hairy roots derived from 4‐week‐old leaves and Agrobacterium rhizogenes strain ATCC15834 were used to investigate the impact of various concentrations of iron oxide nanoparticles (Fe NPs) in two elicitation time exposures (24 and 48 h) on growth, antioxidant enzyme activity, total phenolic and flavonoid content (TPC and TFC), and some polyphenols. Gene expression levels of phenylalanine ammonia‐lyase (pal) and rosmarinic acid synthase (ras) were also analyzed. RESULTS Iron nanoparticles enhanced biomass accumulation in hairy roots. The treatment time and Fe NP dosage largely improved the activity of antioxidant enzymes, TPC and TFC. The highest RA (1194 μg g−1 FW) content (9.7‐fold), compared to controls, was detected with 24 h of exposure to 75 mg L−1 Fe NP, which was consistent with the expression of pal and ras genes under the influence of elicitation. The xanthomicrol, cirsimaritin, and isokaempferide content was increased 11.87, 3.85, and 2.27‐fold, respectively. CONCLUSION Stimulation of D. kotschyi hairy roots by Fe NPs led to a significant increase in the induction and production of important pharmaceutical compounds such as rosmarinic acid and xanthomicrol. © 2019 Society of Chemical Industry

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The comparative effects of nano and bulk size particles of CuO and ZnO on glycyrrhizin and phenolic compounds contents in Glycyrrhiza glabra L. seedlings

Cited by 74 publications

References 22 publications

Major essential oil constituents, total phenolics and flavonoids content and antioxidant activity of Salvia officinalis plant in response to nano-titanium dioxide

Major essential oil constituents, total phenolics and flavonoids content and antioxidant activity of Salvia officinalis plant in response to nano-titanium dioxide

Influence of nano‐zinc oxide on tropane alkaloid production, h6h gene transcription and antioxidant enzyme activity in Hyoscyamus reticulatus L. hairy roots

Iron oxide nanoparticles: a novel elicitor to enhance anticancer flavonoid production and gene expression in Dracocephalum kotschyi hairy‐root cultures

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