The coding potential of circRNAs

Das, Aniruddha; Gorospe, Myriam; Panda, Amaresh C.

doi:10.18632/aging.101554

Cited by 26 publications

(18 citation statements)

References 7 publications

(11 reference statements)

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“…Circular RNAs (circRNAs), a class of covalently closed endogenous RNA molecules, have begun to be extensively studied because of their unique structure and potential function [ 5 ]. In recent years, circRNAs have been reported to serve as sponges on miRNAs, thereby affecting gene transcription or RNA-binding protein interactions [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

Circular RNA hsa_circ_0000073 contributes to osteosarcoma cell proliferation, migration, invasion and methotrexate resistance by sponging miR-145-5p and miR-151-3p and upregulating NRAS

Liu

Zhang

et al. 2020

Aging

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An increasing number of studies have demonstrated that circular RNAs (circRNAs), as promising therapeutic targets, are essential for diverse human diseases, especially cancer. However, the potential functions and complex mechanisms of most circRNAs in osteosarcoma (OS) are still not fully elucidated. In the present study, we obtained the expression profile of circRNAs from a GEO database (GSE96964) and identified hsa_circ_0000073 as a highly expressed candidate in OS. Overexpression of hsa_circ_0000073 accelerated the proliferation, migration, invasion and MTX resistance of OS cells, and knockdown of hsa_circ_0000073 resulted in the opposite effects. Mechanistically, hsa_circ_0000073 upregulated NRAS expression by targeting miR-145-5p and miR-151-3p in OS cells. In addition, the promotion of OS progression by hsa_circ_000007 was blocked by miR-145-5p and miR-151-3p-mediated NRAS inhibition. In conclusion, hsa_circ_0000073 facilitated the proliferation, migration, invasion and MTX resistance of OS cells through the inhibition of miR-145-5p and miR-151-3p-mediated downregulation of NRAS.

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Section: Introductionmentioning

confidence: 99%

Circular RNA hsa_circ_0000073 contributes to osteosarcoma cell proliferation, migration, invasion and methotrexate resistance by sponging miR-145-5p and miR-151-3p and upregulating NRAS

Liu

Zhang

et al. 2020

Aging

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“…CircRNAs, a class of ncRNAs, have emerged as mediators of gene expression. To date, more than 100,000 circRNAs have been identified, which are expressed in specific tissues or cells and are associated with various physiological and pathological conditions ( 17 ). The biogenesis of circRNAs is regulated by various molecular factors such as RNA-binding proteins (RBPs), splicing components, proteins that affect transcriptional elongation, and the presence of reverse RNA repeats ( 18 ).…”

Section: Introductionmentioning

confidence: 99%

Landscape of N6-Methyladenosine Modification Patterns in Human Ameloblastoma

Niu

Liu

et al. 2020

Front. Oncol.

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Objective To comprehensively analyze the global N 6 -methyladenosine (m 6 A) modification pattern in ameloblastoma. Methods m 6 A peaks in ameloblastoma and normal oral tissues were detected by MeRIP-seq. Differentially methylated m 6 A sites within messenger RNAs (mRNAs), long no-coding RNA (lncRNAs) and circular RNA (circRNAs) were identified, followed by functional enrichment analysis. By comprehensively analyzing MeRIP-seq and RNA-seq data, differentially expressed mRNAs, lncRNAs and circRNAs containing differentially methylated sites were identified. RNA binding proteins (RBPs) were then identified for differentially methylated m 6 A sites. Results In total, 3,673 differentially methylated m 6 A sites within coding genes were detected, of which 16.2% (704/3,673) were significantly upmethylated sites in ameloblastoma compared to normal oral tissues. Furthermore, 4,975 differentially methylated m 6 A sites within lncRNAs were identified, of which 29.4% (1,465/4,975) were upmethylated sites in ameloblastoma. We also found 364 differentially methylated m 6 A sites within circRNAs, of which 22.5% (82/364) were upmethylated sites in ameloblastoma. Differentially methylated m 6 A was most often harbored in the CDS (54.10%), followed by 5’UTR (21.71%). Functional enrichment analysis revealed that m 6 A modification could be involved in the development of ameloblastoma by organism developmental processes. A total of 158 RBPs within differentially methylated m 6 A sites were identified, which were significantly involved in mRNA metabolic process, mRNA processing, RNA processing, RNA splicing and RNA transport. Conclusion Our findings for the first time provide m 6 A landscape of human ameloblastoma, which expand the understanding of m 6 A modifications and uncover regulation of lncRNAs and circRNAs through m 6 A modification in ameloblastoma.

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“…Cumulative evidence indicates that ceRNA and circRNAs can function as endogenous sponges to influence mRNA, lncRNA or miRNA activity [27,28]. We found many ncRNAs were significant up-regulated or down-regulated by the mitochondria polymorphism in C. elegans, which include novel miRNAs and lncRNAs and predicted circRNAs and ceRNAs.…”

Section: Agingmentioning

confidence: 68%

The whole transcriptome regulation as a function of mitochondrial polymorphisms and aging in Caenorhabditis elegans

Song

Wang

Zhu

et al. 2020

Aging

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Recently, mitochondrial-nuclear interaction in aging has been widely studied. However, the nuclear genome controlled by natural mitochondrial variations that influence aging has not been comprehensively understood so far. We hypothesized that mitochondrial polymorphisms could play critical roles in the aging process, probably by regulation of the whole-transcriptome expression. Our results showed that mitochondria polymorphisms not only decreased the mitochondrial mass but also miRNA, lncRNA, mRNA, circRNA and metabolite profiles. Furthermore, most genes that are associated with mitochondria show age-related expression features (P = 3.58E-35). We also constructed a differentially expressed circRNA-lncRNA-miRNA-mRNA regulatory network and a ceRNA network affected by the mitochondrial variations. In addition, Kyoto Encyclopedia of Genes and Genomes pathway analyses showed that the genes affected by the mitochondrial variation were enriched in metabolic activity. We finally constructed a multi-level regulatory network with aging which affected by the mitochondrial variation in Caenorhabditis elegans. The interactions between these genes and metabolites have great values for further aging research. In sum, our findings provide new evidence for understanding the molecular mechanisms of how mitochondria influence aging.

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The coding potential of circRNAs

Cited by 26 publications

References 7 publications

Circular RNA hsa_circ_0000073 contributes to osteosarcoma cell proliferation, migration, invasion and methotrexate resistance by sponging miR-145-5p and miR-151-3p and upregulating NRAS

Circular RNA hsa_circ_0000073 contributes to osteosarcoma cell proliferation, migration, invasion and methotrexate resistance by sponging miR-145-5p and miR-151-3p and upregulating NRAS

Landscape of N6-Methyladenosine Modification Patterns in Human Ameloblastoma

The whole transcriptome regulation as a function of mitochondrial polymorphisms and aging in Caenorhabditis elegans

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