The co-effect of surface topography gradient fabricated via immobilization of gold nanoparticles and surface chemistry via deposition of plasma polymerized film of allylamine/acrylic acid on osteoblast-like cell behavior

Liu, Xujie; Xie, Yiyang; Shi, Shengjun; Feng, Qingling; Bachhuka, Akash; Guo, Xiaodong; She, Zhigang; Tan, Rongwei; Cai, Qiang; Vasilev, Krasimir

doi:10.1016/j.apsusc.2018.12.216

Cited by 14 publications

(15 citation statements)

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“…However, both topographical and chemical properties of the surface govern cell differentiation. 190 After culturing osteoblast-like SaOS-2 cells on surfaces, surface nanotopography could enhance the stimulating effects of allylamine chemical treatment on osteogenic differentiation (Fig. 7B).…”

Section: Impact Of Biomaterials Surface Physical Properties On Biologimentioning

confidence: 96%

“…Surface nanofunctionalization has attracted much attention as a promising strategy for enhancing cell responses to biomaterials, which we will address later in this paper. Liu et al 190 designed some surface nanotopography gradients through surface immobilization of gold nanoparticles in a density-dependent manner. They modified the surface chemistry of scaffolds via coating a thin plasma polymerized film with allylamine (AA) or acrylic acid (AC) chemical composition on the surface (Fig.…”

Section: Impact Of Biomaterials Surface Physical Properties On Biologimentioning

confidence: 99%

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Biological responses to physicochemical properties of biomaterial surface

et al. 2020

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Section: Impact Of Biomaterials Surface Physical Properties On Biologimentioning

confidence: 96%

Section: Impact Of Biomaterials Surface Physical Properties On Biologimentioning

confidence: 99%

Biological responses to physicochemical properties of biomaterial surface

et al. 2020

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“…It should be noticed that the AuNPs employed here to generate a surface topography gradient are chemically inert and do not cause adverse cellular response. 16 AuNPs are one of the most extensively explored nanomaterials and have been widely applied in biomedicine. 47 Moreover, the strong electrostatic interaction and the pinhole-free plasma-polymerized thin film on top of AuNPs both can prevent AuNPs from penetrating the cells cultured on the substrates.…”

Section: Discussionmentioning

confidence: 99%

“…23,24 More importantly, cells growing on gradient surfaces can have a certain degree of "selection" rather than "adaptation" to surface features, even if they are not the preferred features. 16 Herein, hMSCs were cultured on these model substrates with defined surface chemistry and surface topography gradient. The morphology and focal adhesion (FA) formation of hMSCs were observed.…”

Section: Introductionmentioning

confidence: 99%

Synergistic Effect of Surface Chemistry and Surface Topography Gradient on Osteogenic/Adipogenic Differentiation of hMSCs

Liu

Wang

et al. 2021

ACS Appl. Mater. Interfaces

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Much attention has been paid to understanding the individual effects of surface chemistry or topography on cell behavior. However, the synergistic influence of both surface chemistry and surface topography on differentiation of human mesenchymal stem cells (hMSCs) should also be addressed. Here, gold nanoparticles were immobilized in an increasing number density manner to achieve a surface topography gradient; a thin film rich in amine (−NH 2 ) or methyl (−CH 3 ) chemical groups was plasma-polymerized to adjust the surface chemistry of the outermost layer (ppAA and ppOD, respectively). hMSCs were cultured on these model substrates with defined surface chemistry and surface topography gradient. The morphology and focal adhesion (FA) formation of hMSCs were first examined. hMSC differentiation was then co-induced in osteogenic and adipogenic medium, as well as in the presence of extracellular-signal-regulated kinase1/2 (ERK1/2) and RhoA/Rhoassociated protein kinase (ROCK) inhibitors. The results show that the introduction of nanotopography could enhance FA formation and osteogenesis but inhibited adipogenesis on both ppAA and ppOD surfaces, indicating that the surface chemistry could regulate hMSC differentiation, in a surface topography-dependent manner. RhoA/ROCK and ERK1/2 signaling pathways may participate in this process. This study demonstrated that surface chemistry and surface topography can jointly affect cell morphology, FA formation, and thus osteogenic/adipogenic differentiation of hMSCs. These findings highlight the importance of the synergistic effect of different material properties on regulation of cell response, which has important implications in designing functional biomaterials.

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“…One of the biggest problems with medical devices is fibrosis or fibrous encapsulation. − Several strategies involving addition of growth factors, − surface chemical modifications, ,− or the addition of surface nanotopography ,− have been explored to address these problems. The data presented here suggest that the combinatorial effect of surface nanoporosity and surface chemistry can be used to control macrophage differentiation by modulating pro-inflammatory and anti-inflammatory cytokines.…”

Section: Discussionmentioning

confidence: 99%