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“…In the rabbit, a serum-leukotactic activ ity has been reported to be due to clot for mation [20], and chemotactic fibrinopeptides have been characterized after interac tion of proteases with human fibrinogen [12] or rabbit fibrin [19], In accordance with Hausman et al [7] in the guinea pig, a correlation of clotting and chemotactic ac tivity was not found in the human. As figure 6 demonstrates, normal serum, normal he parinized plasma, and serum prepared from plasma which had been obtained by fast centrifugation of whole blood, all showed a similar dose-response pattern for monocytechemotactic activity.…”
Section: Clotting and Fibrinolytic Systemmentioning
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“…In the rabbit, a serum-leukotactic activ ity has been reported to be due to clot for mation [20], and chemotactic fibrinopeptides have been characterized after interac tion of proteases with human fibrinogen [12] or rabbit fibrin [19], In accordance with Hausman et al [7] in the guinea pig, a correlation of clotting and chemotactic ac tivity was not found in the human. As figure 6 demonstrates, normal serum, normal he parinized plasma, and serum prepared from plasma which had been obtained by fast centrifugation of whole blood, all showed a similar dose-response pattern for monocytechemotactic activity.…”
Section: Clotting and Fibrinolytic Systemmentioning
“…However, it was recently found that D-glucose induces a significantly stronger chemokinetic effect on neutrophils from the chronically hyperglycemic Umeå ob/ob mouse, compared with control mice [35]. In this work, neutrophil locomotion on glass was studied in cells moving on a complex substratum known to activate neutrophil locomotion [24] or in gradientpurified neutrophils moving on BSA-coated glass. D-Glucose induced about the same absolute increase in chemokinesis in neutrophils isolated with these two techniques.…”
Section: Discussionmentioning
“…Because blood coagulation is known to generate chemotactic substances [24], this phenomenon was used to obtain basically activated neutrophils (blood clot-isolated). These were compared with neutrophils isolated with a gradient technique, generating non-activated neutrophils (gradientisolated).…”
Section: Chemokinetic Effects Of Glucose On Neutrophil Random Locomotionmentioning
“…Generally, neutrophils accumulate in tissues in response to chemotactic factors associated with tissue injury/inflammatory processes 27 and products of hemostatic activity. 28 Under these conditions activated neutrophils can release substances noxious to the vascular endothelium and can produce capillary plugging that exacerbates injury. 29 - 31 However, to initiate this process the integrin complex of adhesion glycoproteins, CD18, is required for leukocyte adherence to endothelium.…”
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