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Cited by 17 publications
(8 citation statements)
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“…28 Single enzyme molecule assay was studied by incubating the enzyme molecule in the substrate solution filled in the capillary. [32][33][34][35] Inhibitor dissociation constant of an enzyme molecule of -galactosidase was also studied by stopping the applied voltage and incubation. 36 In this study, a different format of EMMA was examined.…”
Section: Introductionmentioning
confidence: 99%
“…28 Single enzyme molecule assay was studied by incubating the enzyme molecule in the substrate solution filled in the capillary. [32][33][34][35] Inhibitor dissociation constant of an enzyme molecule of -galactosidase was also studied by stopping the applied voltage and incubation. 36 In this study, a different format of EMMA was examined.…”
Section: Introductionmentioning
confidence: 99%
“…Irradiating the higher field absorption (6 2.67 ppm) spindecoupled the lower vinyl triplet (6 4.61 ppm), whereas irradiation of the lower field absorption (6 3.28 ppm) caused the same effect upon the higher vinyl triplet (6 4.40 ppm). This correlation between allyl and vinyl absorptions in the spectrum of 9 does not yet allow one to make an unequivocal proton assignment of the respective ally1 and vinyl absorptions. Such an assignment can be done, however, after examining the NMR spectrum of 11.…”
Section: H -N M R Spectra Ofthe Tricyc10[4220~~ldecane Systemmentioning
confidence: 96%
“…This may be due to the fact that this mode typically does not produce sharp substance peaks but rather broad zones. Nonetheless, Craig and coworkers published a series of applications of continuous CE assays for the measurement of individual molecules of β-galactosidase using 9H-(1,3-dichloro-9,9-dimethylacridin-2-one-7yl)-β-d-galactopyranoside (DDAO-gal) as substrate [95][96][97][98][99][100][101][102]. In the so-called double static incubation assay, 50 cm, 5 μm id, fused-silica capillaries were filled with buffer containing the substrate and highly diluted enzyme, so that on average only 5-10 enzyme molecules were distributed throughout the capillary.…”
Section: In-capillary Enzyme Assaysmentioning
confidence: 99%
“…Following incubation, the content of the capillary was mobilized by application of a voltage and the fluorescent product, DDAO, was detected at the cathodic end. Because the enzymes were several centimeters apart due to the high dilution, the product zones represented the activity of individual enzymes [95]. In the continuous flow set-up, the capillary was filled with the fluorogenic substrate and very dilute enzyme was continuously mobilized through the capillary.…”
Section: In-capillary Enzyme Assaysmentioning
confidence: 99%
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