The CH<SUB>2</SUB>Cl<SUB>2</SUB><i> </i>Extract Fraction from<i> Ficus erecta</i> var. <i>sieboldii</i> (Miq.) King Suppresses Lipopolysaccharide-mediated Inflammatory Responses in Raw264.7 Cells

Abstract

A phytochemical application of leaves from Ficus erecta var. sieboldii (Miq.) King has not been widely investigated. We determined an anti-inflammatory effect of F. erecta extracts on lipopolysaccharide (LPS)-mediated production through modulation of several pro-inflammatory mediators and prostaglandin E2 (PGE 2). Among the F. erecta extracts, the CH 2 Cl 2 fraction (CFE) most effectively suppressed the LPS-mediated production of nitric oxide (NO) in Raw264.7 cells. As determined by immunoblotting and PCR, CFE was shown to have an inhibitory effect on LPS-induced mRNA and protein expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2). In addition, CFE showed significant inhibitory effects on LPS-mediated production of tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6, and PGE 2 (P<0.05), demonstrating its effects on inflammation. The main active compounds that suppressed PGE 2 production were syringaresinol (C1) and 6,7-furano-5-methoxy hydrocoumaric acid (C2). In conclusion, CFE showed an inhibitory effect on LPS-mediated inflammatory responses by suppressing iNOS, COX-2, TNF-α, IL-1β, and IL-6 production. The compounds C1 and C2 showed strong inhibitory effects on LPS-mediated production of PGE 2 and may be applicable as starter compounds for developing anti-inflammatory and anti-nociceptive drugs.

Enhancement of Immune Activities of Mixtures with Sasa quelpaertensis Nakai and Ficus erecta var. sieboldii

“…For a long time, it has been known to be effective in promoting rejuvenation, bone health, wound recovery, blood circulation, and detoxification [8]. In recent studies, the potential of FES as a functional food was reported by evaluating its catechin content, antioxidant activity, and anti-inflammatory effects in a hypersensitivity-free macrophage model [9,10]. Sasamorpha has been reported to be widely distributed in Asian countries such as Korea, China, and Japan [11].…”