The carbonic anhydrase I locus contains a c-Myb target promoter and modulates differentiation of murine erythroleukemia cells

Chen, J; Kremer, Christopher S; Bender, Timothy P.

doi:10.1038/sj.onc.1209295

Cited by 19 publications

(18 citation statements)

References 79 publications

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“…Every coin has two sides, and the transcription factor MYB, also known as a proto-oncogene, operates as a transcriptional activator as well as transcriptional repressor. [30][31][32][33] Hitherto, some evidence has strengthened the case that MYB can transactivate Bcl-2 by recognizing the transcription factor binding site (TFBS). 34,35 More excitingly, the data from our study revealed that MYB functions as a transcriptional repressor by inhibiting miR-148a, which could also silence Bcl-2.…”

Section: Discussionmentioning

confidence: 99%

MiR-148a promotes apoptosis by targeting Bcl-2 in colorectal cancer

et al. 2011

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Section: Discussionmentioning

confidence: 99%

MiR-148a promotes apoptosis by targeting Bcl-2 in colorectal cancer

et al. 2011

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“…Surprisingly, in our study, CAI has been found to be significantly downregulated in the rumen after 6 wk of concentrate feeding. Notably, in addition to its well-characterized function in pH regulation and ion transport, CAI is also a c-Myb target involved in regulating cell proliferation and differentiation (12,34,50). Since high-concentrate diet leads to an increase in the size and number of ruminal papillae (18), we propose that the decreased expression of CAI might be linked to the c-Myb pathway.…”

Section: G265 Diet-dependent Protein Expression Of Ruminal Epitheliummentioning

confidence: 99%

Identification of differentially expressed proteins in ruminal epithelium in response to a concentrate-supplemented diet

Bondzio¹,

Gabler²,

Badewien-Rentzsch³

et al. 2011

American Journal of Physiology-Gastrointestinal and Liver Physi

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minal epithelium adapts to dietary change with well-coordinated alterations in metabolism, proliferation, and permeability. To further understand the molecular events controlling diet effects, the aim of this study was to evaluate protein expression patterns of ruminal epithelium in response to various feeding regimes. Sheep were fed with a concentrate-supplemented diet for up to 6 wk. The control group received hay only. Proteome analysis with differential in gel electrophoresis technology revealed that, after 2 days, 60 proteins were significantly modulated in ruminal epithelium in a comparison between hay-fed and concentrate-fed sheep (P Ͻ 0.05). Forty proteins were upregulated and 20 proteins were downregulated in response to concentrate diet. After 6 wk of this diet, only 14 proteins were differentially expressed. Among these, 11 proteins were upregulated and 3 downregulated. To identify proteins that were modulated by dietary change, two-dimensional electrophoresis was coupled with liquid chromatography electrospray ionization mass spectrometry. The differential expression of selected proteins, such as esterase D, annexin 5, peroxiredoxin 6, carbonic anhydrase I, and actin-related protein 3, was verified by immunoblotting and/or mRNA analysis. The identified proteins were mainly associated with functions related to cellular stress, metabolism, and differentiation. These results suggest new candidate proteins that may contribute to a better understanding of the signaling pathways and mechanisms that mediate rumen epithelial adaptation to high-concentrate diet.proteomics; mRNA expression; protein markers; sheep RUMINANTS ARE OF MAJOR WORLDWIDE interest and economic importance because of increasing meat and milk production. A substantial improvement in the productivity and performance of ruminants can only be achieved with adequate dietary supplementation. When high-yielding cattle, milk sheep, or goats merely consume forage, the intake of energy and protein is too low for the desired rates of animal performance. Hence, supplementation of forage-fed ruminants with sufficient amounts of energy and protein is necessary and significantly improves milk production or daily weight gain (41), but, particularly, a surplus of easily fermented carbohydrates or low-fiber intake will place the animals at risk. Such a change in the diet is a challenge for rumen microbes (31) and especially for the ruminal epithelium with respect to adaptation to the new fermentation pattern (47,55). Insufficient adaptation to diet may result in disintegration of the epithelium (22), translocation of lipopolysaccharides (30), and disturbed transport mechanisms (22) and can further cause subacute ruminal acidosis (14, 49). This disease not only can lead to depressed feed intake and milk production, but can also be associated with laminitis, inflammation, and liver abscesses (14,42,49).Hence, one objective of attaining an adequate diet composition for ruminants is to maintain the physiological functions of the forestomach, such as optimal fe...

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“…The data from our study show that CA-I is initially up-regulated in erythroblasts between days 3 and 6 in culture and is expressed from then on throughout erythropoiesis. Interestingly, it has been suggested that CA-I may play a physiological role in erythroid differentiation [42,43], although its precise role is presently unclear.…”

Section: Discussionmentioning

confidence: 99%

Analysis of the differential proteome of human erythroblasts during in vitro erythropoiesis by 2‐D DIGE

Richardson

Heesom

Parsons

et al. 2009

Proteomics Clinical Apps

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In the present study we have used an in vitro culture system that induces differentiation of human CD341 cells down the erythroid lineage along with 2-D DIGE to determine the differential proteome of erythroblasts at specific developmental stages during erythropoiesis. We initially distinguished 154 proteins differentially expressed between pro-normoblasts and polychromatic/orthochromatic erythroblasts, of which 24 protein spots, representing 21 different proteins, were identified following MS/MS and verification in replicate experiments with cells from different individuals. These data were confirmed by analysis of the differential proteome of erythroblasts at more discrete stages of erythropoiesis using 2-D DIGE and by mapping the expression of three identified proteins (Annexin I, Annexin II, Carbonic Anhydrase I) throughout erythropoiesis by Western blot with specific antisera. In addition, the differential expression of proteins due to biological variation, such as polymorphism, was determined by comparing erythroblasts at the same developmental stage from different individuals; none of the proteins thus identified were represented in the above data set. Finally, we discuss the problems associated with 2-D DIGE gel-based proteomic approaches such as ours and suggest a modified approach for decreased inter-gel variation, improved protein resolution and increased protein concentration, which should significantly facilitate protein identification.

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The carbonic anhydrase I locus contains a c-Myb target promoter and modulates differentiation of murine erythroleukemia cells

Cited by 19 publications

References 79 publications

MiR-148a promotes apoptosis by targeting Bcl-2 in colorectal cancer

MiR-148a promotes apoptosis by targeting Bcl-2 in colorectal cancer

Identification of differentially expressed proteins in ruminal epithelium in response to a concentrate-supplemented diet

Analysis of the differential proteome of human erythroblasts during in vitro erythropoiesis by 2‐D DIGE

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