The C-X-C signalling system in the rodent vs primate testis: impact on germ cell niche interaction

Heckmann, Laura; Pock, Tim; Tröndle, Ina; Neuhaus, Nina

doi:10.1530/rep-17-0617

Cited by 13 publications

(5 citation statements)

References 66 publications

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“…Within the testis, a special situation may exist, since MIF is a ligand for the CXCR4, expressed by SSCs, and is essential for postnatal maintenance of the SSCs in marmosets and mice 57 . If indeed applicable to the testis, higher levels of MIF may influence the SSC niche via CXCR4 binding.…”

Section: Discussionmentioning

confidence: 99%

Insights into replicative senescence of human testicular peritubular cells

Schmid

Flenkenthaler²,

Stöckl³

et al. 2019

Sci Rep

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There is evidence for an age-related decline in male reproductive functions, yet how the human testis may age is not understood. Human testicular peritubular cells (HTPCs) transport sperm, contribute to the spermatogonial stem cell (SSC) niche and immune surveillance, and can be isolated and studied in vitro. Consequences of replicative senescence of HTPCs were evaluated to gain partial insights into human testicular aging. To this end, early and advanced HTPC passages, in which replicative senescence was indicated by increased cell size, altered nuclear morphology, enhanced β-galactosidase activity, telomere attrition and reduced mitochondrial DNA (mtDNA), were compared. These alterations are typical for senescent cells, in general. To examine HTPC-specific changes, focused ion beam scanning electron microscopy (FIB/SEM) tomography was employed, which revealed a reduced mitochondrial network and an increased lysosome population. The results coincide with the data of a parallel proteomic analysis and indicate deranged proteostasis. The mRNA levels of typical contractility markers and growth factors, important for the SSC niche, were not significantly altered. A secretome analysis identified, however, elevated levels of macrophage migration inhibitory factor (MIF) and dipeptidyl peptidase 4 (DPP4), which may play a role in spermatogenesis. Testicular DPP4 may further represent a possible drug target.

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Section: Discussionmentioning

confidence: 99%

Insights into replicative senescence of human testicular peritubular cells

Schmid

Flenkenthaler²,

Stöckl³

et al. 2019

Sci Rep

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show abstract

“…Previously, we described an important role of CXCR4 in mouse SSC maintenance [22]. Interestingly, recent studies reported that CXCR4 + cells contained enriched SSC activity in bull, boar and human testis [32, 43, 44] . Findings from the current study demonstrated that CXCR4 was only expressed in gonocytes and a small number of spermatogonia; we therefore proposed that CXCR4 can be used as a marker for SSC enrichment.…”

Section: Discussionmentioning

confidence: 99%

Gene expression dynamics during the gonocyte to spermatogonia transition and spermatogenesis in the domestic yak

Wang

et al. 2019

J Animal Sci Biotechnol

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Background Spermatogenesis is a cellular differentiation process that includes three major events: mitosis of spermatogonia, meiosis of spermatocytes and spermiogenesis. Steady-state spermatogenesis relies on functions of spermatogonial stem cells (SSCs). Establishing and maintaining a foundational SSC pool is essential for continued spermatogenesis in mammals. Currently, our knowledge about SSC and spermatogenesis is severely limited in domestic animals. Results In the present study, we examined transcriptomes of testes from domestic yaks at four different stages (3, 5, 8 and 24 months of age) and attempted to identify genes that are associated with key developmental events of spermatogenesis. Histological analyses showed that the most advanced germ cells within seminiferous tubules of testes from 3, 5, 8 and 24 months old yaks were gonocytes, spermatogonia, spermatocytes and elongated spermatids, respectively. RNA-sequencing (RNA-seq) analyses revealed that 11904, 4381 and 2459 genes were differentially expressed during the gonocyte to spermatogonia transition, the mitosis to meiosis transition and the meiosis to post-meiosis transition. Further analyses identified a list of candidate genes than may regulate these important cellular processes. CXCR4 , a previously identified SSC niche factor in mouse, was one of the up-regulated genes in the 5 months old yak testis. Results of immunohistochemical staining confirmed that CXCR4 was exclusively expressed in gonocytes and a subpopulation of spermatogonia in the yak testis. Conclusions Together, these findings demonstrated histological changes of postnatal testis development in the domestic yak. During development of spermatogonial lineage, meiotic and haploid germ cells are supported by dynamic transcriptional regulation of gene expression. Our transcriptomic analyses provided a list of candidate genes that potentially play crucial roles in directing the establishment of SSC and spermatogenesis in yak. Electronic supplementary material The online version of this article (10.1186/s40104-019-0360-7) contains supplementary material, which is available to authorized users.

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“…As a chemokine involved in the regulation of tissue stem cells, the previous analyses of CXCL12 have been impressive. CXCL12, through its receptor CXCR4, is responsible for the maintenance and differentiation of tissue stem cells, such as bone marrow hematopoietic stem cells, primordial germ cells, and neural stem cells, in the stem cell niche of the tissue microenvironment [ 56 , 57 , 58 ]. This function may be mainly based on the chemoattractant activity of CXCL12 toward the stem cell niche.…”

Section: Discussionmentioning

confidence: 99%

CCL28: A Promising Biomarker for Assessing Salivary Gland Functionality and Maintaining Healthy Oral Environments

Kaibori,

Tamoto,

Okuda

et al. 2024

Biology

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The oral cavity serves as the primary path through which substances from the outside world enter our body. Therefore, it functions as a critical component of host defense. Saliva is essential for maintaining a stable oral environment by catching harmful agents, including pathogens, allergens, and chemicals, in the air or food. CCL28, highly expressed in mucosal tissues, such as the colon and salivary glands, is a chemokine that attracts CCR10/CCR3 expressing cells. However, the role of CCL28 in salivary gland formation remains unclear. In this study, we investigated the salivary gland structure in CCL28-deficient mice. Histological analysis showed decreased staining intensity of Alcian blue, which detects acidic mucous, reduced expression of MUC2, and higher infiltration of gram-positive bacteria in the salivary glands of CCL28-deficient mice. In addition, CCL28-deficient mice contained ectopically MUC2-expressed cells in the ducts and reduced the expression of cytokeratin 18, a marker for ductal cells, within the submandibular glands, resulting in decreased duct numbers. Additionally, the submandibular glands of CCL28-deficient mice showed reduced expression of several stem cell markers. These results suggest that CCL28 regulates saliva production via proper differentiation of salivary gland stem cells and could be a valuable biomarker of salivary gland function.

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The C-X-C signalling system in the rodent vs primate testis: impact on germ cell niche interaction

Cited by 13 publications

References 66 publications

Insights into replicative senescence of human testicular peritubular cells

Insights into replicative senescence of human testicular peritubular cells

Gene expression dynamics during the gonocyte to spermatogonia transition and spermatogenesis in the domestic yak

CCL28: A Promising Biomarker for Assessing Salivary Gland Functionality and Maintaining Healthy Oral Environments

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