The Bradford Method for Protein Quantitation

Kruger, Nicholas J.

doi:10.1385/0-89603-268-x:9

Cited by 506 publications

(253 citation statements)

References 14 publications

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“…The target fractions were analysed by SDS-PAGE, pooled and concentrated using an Amicon Ultra-15 centrifugal filter unit (molecular mass cut-off, 30 kDa; Millipore) to change the buffer to 20 mM Tris/HCl (pH 7.5). Protein concentration was determined by the method of Bradford (Kruger, 1994). Cells from two independent colonies were grown in MMP with the compounds indicated as the sole source of carbon and nitrogen (20 mM) except for GA (10 mM) where 0.5 % NH 4 Cl was added as the nitrogen source.…”

Section: Methodsmentioning

confidence: 99%

Molecular characterization of lysR-lysXE, gcdR-gcdHG and amaR-amaAB operons for lysine export and catabolism: a comprehensive lysine catabolic network in Pseudomonas aeruginosa PAO1

Indurthi

Chou

2016

Microbiology

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Section: Methodsmentioning

confidence: 99%

Molecular characterization of lysR-lysXE, gcdR-gcdHG and amaR-amaAB operons for lysine export and catabolism: a comprehensive lysine catabolic network in Pseudomonas aeruginosa PAO1

Indurthi

Chou

2016

Microbiology

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“…The protein content (free HBsAg) supernatant was analyzed by the Bradford protein assay. 28,29 The HBsAg loading efficiency (LE) and loading capacity (LC) of the nanoparticles were estimated as follows:…”

Section: Hbsag Loading Efficiency and Loading Capacity Of Nanoparticlesmentioning

confidence: 99%

Enteric trimethyl chitosan nanoparticles containing hepatitis B surface antigen for oral delivery

Farhadian

Dounighi

Avadi³

2015

Human Vaccines & Immunotherapeutics

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Oral vaccination is the preferred route of immunization. However, the degradative condition of the gastrointestinal tract and the higher molecular size of peptides pose major challenges in developing an effective oral vaccination system. One of the most excellent methods used in the development of oral vaccine delivery system relies on the entrapment of the antigen in polymeric nanoparticles. In this work, trimethyl chitosan (TMC) nanoparticles were fabricated using ionic gelation teqnique by interaction hydroxypropyl methylcellulose phthalate (HPMCP), a pHsensitive polymer, with TMC and the utility of the particles in the oral delivery of hepatitis B surface antigen (HBsAg) was evaluated employing solutions that simulated gastric and intestinal conditions. The particle size, morphology, zeta potential, loading capacity, loading efficiency, in vitro release behavior, structure, and morphology of nanoparticles were evaluated, and the activity of the loaded antigen was assessed. Size of the optimized TMC/HPMCP nanoparticles and that of the antigen-loaded nanoparticles were 85 nm and 158 nm, respectively. Optimum loading capacity (76.75%) and loading efficiency (86.29%) were achieved at 300 mg/mL concentration of the antigen. SEM images revealed a spherical shape as well as a smooth and near-homogenous surface of nanoparticles. Results of the in vitro release studies showed that formulation with HPMCP improved the acid stability of the TMC nanoparticles as well as their capability to preserve the loaded HBsAg from gastric destruction. The antigen showed good activity both before and after loading. The results suggest that TMC/HPMCP nanoparticles could be used in the oral delivery of HBsAg vaccine.

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“…The duration of the reaction between the Coomassie Brilliant Blue solution and the sample was 20 minutes. After all, using the Specol 2000 (Poland) and wavelength equal to 590nm, we have determined the concentration of the total proteins in the biological solution [7].…”

Section: Detection and Quantification Of The Proteins By Bradfordmentioning

confidence: 99%

Subcomponents of Vitamine B Complex Regulate the Growth and Development of Human Brain Derived Cells

Danielyan¹

2013

AJBR

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The work is focused on the role of single components of Vitamin B complex: pyridoxine, riboflavin, thiamine, and nicotinamide in the processes of growth and development of the human brain derived cells. We, also, have assayed the activity of Xanthine Oxidase in the presence of above mentioned subcomponents to delineate the possible mechanism of their action. Results indicate that the all components of Vitamin B complex might be responsible for cells' growth, maturation, proliferation. During early period of the cells' growth the most important components were thiamine and Pyridoxine, initiating cells' proliferation (number of the cells in one field: 2556, 17±355, 68, 2179,0±223,55, resp) vs control (1562,94±146,45), whereas during the late stages of maturation the most important components responsible for differentiation, were riboflavin and nicotinamide (3774.77± 188.41, 3558.82±152.90 resp. vs control 2905±263.75; p<0.035). In comparison with the all other subcomponents of Vitamin B complex only in pyridoxine containing samples, XO activity was specifically inhibited by allopurinol (percentile of inhibition 160,00±60,00 vs control 31,03±6,92, p<0,05). We have concluded that Pyridoxine might interact with XO and regulate its activity. All components of Vitamin B complex are able to initiate cells development and growth; however, they are supposed to be selectively utilized in time dependent manner to guarantee the highest efficiency of the cells development, maturation and proliferation

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The Bradford Method for Protein Quantitation

Cited by 506 publications

References 14 publications

Molecular characterization of lysR-lysXE, gcdR-gcdHG and amaR-amaAB operons for lysine export and catabolism: a comprehensive lysine catabolic network in Pseudomonas aeruginosa PAO1

Molecular characterization of lysR-lysXE, gcdR-gcdHG and amaR-amaAB operons for lysine export and catabolism: a comprehensive lysine catabolic network in Pseudomonas aeruginosa PAO1

Enteric trimethyl chitosan nanoparticles containing hepatitis B surface antigen for oral delivery

Subcomponents of Vitamine B Complex Regulate the Growth and Development of Human Brain Derived Cells

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