The Biocontrol Potential of Endophytic Trichoderma Fungi Isolated from Hungarian Grapevines. Part I. Isolation, Identification and In Vitro Studies

Kovács, Csilla; Csótó, András; Pál, Károly; Nagy, Antal; Fekete, Erzsébet; Karaffa, Levente; Kubicek, Christian P.; Sándor, Erzsébet

doi:10.3390/pathogens10121612

Cited by 13 publications

(21 citation statements)

References 58 publications

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“…Introduction of new biocontrol strains in the form of Trichoderma has several benefits to act as a biostimulant and plant growth promoters. However, weather variability also needs to be considered in long run for the management of emerging pathogens recently (Kovács et al, 2021 ). New biocontrol agents should have potential effectiveness to inhibit the pathogen under varying weather and climatic conditions (Modrzewska et al, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

Potential biocontrol efficiency of Trichoderma species against oomycete pathogens

Liu

He²,

Munir³

et al. 2022

Front. Microbiol.

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Plant health is of utmost importance for optimal agricultural production and sustainability. Unfortunately, biotic and abiotic factors put a major constraint on crop safety and productivity. Plant diseases caused by oomycetes inflict serious damage to various crops. Moreover, the injudicious use of chemical pesticides poses threats related to pesticide resistance development in pathogens and environmental pollution. Biocontrol offers an effective solution for disease control; however, research on biocontrol of oomycete-related diseases is scarce. Thus, this study undertakes the screening of biocontrol resources for the effective management of oomycete-related plant diseases. In this regard, 86 isolates of Trichoderma spp. were assessed against Phytophthora nicotianae, P. capsici, Pythium vexans, P. ultimum, and P. dissotocum through dual culture assay. Furthermore, the antagonistic effect of selected isolates was studied against tobacco black shank disease and damping-off of cucumber seedlings in the greenhouse. The relative control effect of the three antagonistic Trichoderma strains AR-4, Tv-1, and ST4-1 on tobacco black shank was more than 60%, which was not significantly different from 6.88 gl−1 fluopicolide–propamocarb. Whereas, the relative control effect of Trichoderma AR-4 and ST4-1 on damping-off of cucumber seedlings was 80.33% and 82.67%, respectively, which were significantly higher than Trichoderma Tv-1 (35.49%) and fluopicolide–propamocarb (47.82%). According to the morphological and molecular characterization, the fungal strains AR-4, Tv-1, and ST4-1 were identified as Trichoderma koningiopsis, T. asperellum, and T. gamsii, respectively. In conclusion, the strains exhibited a strong antagonistic effect against oomycete pathogens and can be integrated into disease management strategies.

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Section: Introductionmentioning

confidence: 99%

Potential biocontrol efficiency of Trichoderma species against oomycete pathogens

Liu

He²,

Munir³

et al. 2022

Front. Microbiol.

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“…According to Rush et al [64], Trichoderma species represent 50-60% of the fungal BCAs. Currently, at least 77 commercial Trichoderma-based biofungicides are available on the global market, including 7 approved by the European Commission for use in the Member States of the European Union (Supplementary Table S1) [18,[64][65][66][67][68][69][70][71][72]. The above commercial preparations contain at least 36 different strains belonging to 13 species of the Trichoderma: T. asperellum (9), T. afroharzianum (formerly T. harzianum, 1), T. atroviride (8), T. atrobrunneum (1), T. asperelloides (1), T. fertile (1), T. gamsii (1), T. harzianum (7), T. hamatum (1), T. polysporum (1), T. stromaticum (1), T. virens (1), and T. viride (3), applied separately or in a consortium (Supplementary Table S1).…”

Section: Characteristics Of the Trichoderma Allowing Its Use In Agric...mentioning

confidence: 99%

Trichoderma: The Current Status of Its Application in Agriculture for the Biocontrol of Fungal Phytopathogens and Stimulation of Plant Growth

Tyśkiewicz

Nowak

Ozimek

et al. 2022

IJMS

243

136

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Rhizosphere filamentous fungi of the genus Trichoderma, a dominant component of various soil ecosystem mycobiomes, are characterized by the ability to colonize plant roots. Detailed knowledge of the properties of Trichoderma, including metabolic activity and the type of interaction with plants and other microorganisms, can ensure its effective use in agriculture. The growing interest in the application of Trichoderma results from their direct and indirect biocontrol potential against a wide range of soil phytopathogens. They act through various complex mechanisms, such as mycoparasitism, the degradation of pathogen cell walls, competition for nutrients and space, and induction of plant resistance. With the constant exposure of plants to a variety of pathogens, especially filamentous fungi, and the increased resistance of pathogens to chemical pesticides, the main challenge is to develop biological protection alternatives. Among non-pathogenic microorganisms, Trichoderma seems to be the best candidate for use in green technologies due to its wide biofertilization and biostimulatory potential. Most of the species from the genus Trichoderma belong to the plant growth-promoting fungi that produce phytohormones and the 1-aminocyclopropane-1-carboxylate (ACC) deaminase enzyme. In the present review, the current status of Trichoderma is gathered, which is especially relevant in plant growth stimulation and the biocontrol of fungal phytopathogens.

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“…Endophytic Trichoderma strains were isolated from white grapevine plants (Furmint cultivar) from the Tokaj Wine Region, Northeast Hungary ( Figure 1 ). The isolation and characterization of the T. afroharzianum (TR04), T. simmonsii (TR05) , T. orientale (TR06), and T. gamsii (TR08) strains that were used in this study were previously described by Kovács et al [ 37 ].…”

Section: Methodsmentioning

confidence: 99%

“…The method previously described in Kovács at al. [ 37 ] was followed for the isolation of fungi from the inner, woody part of the plants, as well as from different parts of the grapevine plants ( Figure 2 ).…”

Section: Methodsmentioning

confidence: 99%

“…We recently described and characterized endophytic Trichoderma strains from grapevines [ 37 ]. In this paper we report and discuss the ability of two different mixes of some of these isolates ( T. simmonsii, T. orientale and T. gamsii , as well as T. afroharzianum and T. simmonsii ) for the long-term colonization of plants and stimulatory potential on different grapevine cultivars in commercial vineyards.…”

Section: Introductionmentioning

confidence: 99%

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The Biocontrol Potential of Endophytic Trichoderma Fungi Isolated from Hungarian Grapevines, Part II, Grapevine Stimulation

et al. 2022

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In the first part of this two-piece publication, the isolation, identification and in vitro characterization of ten endophytic Trichoderma isolates were reported. Here we report the ability of two different mixes of some of these isolates (Trichoderma simmonsii, Trichoderma orientale and Trichoderma gamsii as well as of Trichoderma afroharzianum and T. simmonsii) to colonize and stimulate the growth of grapevines. Two commercial vineyards about 400 km away from the site of isolation were used as experimental fields, from which the strains of three Trichoderma species were re-isolated up to four years after rootstock soaking treatment with conidiospores, performed before planting. The treatments decreased the overall percentage of lost plants of about 30%, although a low number of lost plants (about 5%) were observed also in the control plot. For all cultivars and clones, the Trichoderma treatments significantly increased both the bud burst ratio and bud burst vigor index. In addition, the grape must parameters such as the Brix degrees, as well as the extract, the D-glucose and the D-fructose concentrations all appeared to be improved, suggesting a potentially higher ethanol content of the produced wine. We conclude that grapevine-endophytic Trichoderma isolates promote plant growth, and this feature represent a very interesting aspect to be deepened, because useful for the integrated plant production in the actual contest on the sustainable agriculture.

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The Biocontrol Potential of Endophytic Trichoderma Fungi Isolated from Hungarian Grapevines. Part I. Isolation, Identification and In Vitro Studies

Cited by 13 publications

References 58 publications

Potential biocontrol efficiency of Trichoderma species against oomycete pathogens

Potential biocontrol efficiency of Trichoderma species against oomycete pathogens

Trichoderma: The Current Status of Its Application in Agriculture for the Biocontrol of Fungal Phytopathogens and Stimulation of Plant Growth

The Biocontrol Potential of Endophytic Trichoderma Fungi Isolated from Hungarian Grapevines, Part II, Grapevine Stimulation

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