The biocompatibility of materials used in printed circuit board technologies with respect to primary neuronal and K562 cells

Mazzuferi, Manuela; Bovolenta, Roberta; Bocchi, Massimo; Braun, T.; Bauer, J.; Jung, E.; Iafelice, Bruno; Guerrieri, R.; Destro, Federica; Borgatti, Monica; Bianchi, Nicoletta; Simonato, Michele; Gambari, Roberto

doi:10.1016/j.biomaterials.2009.10.025

Cited by 16 publications

(14 citation statements)

References 30 publications

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“…In the past few years, PCB technology has reached a resolution of tens of micrometers and some studies have been conducted to demonstrate the biocompatibility of a wide variety of materials used in PCB manufacturing processes in order to include this technology as a feasible option in the construction of lab-on-a-chip devices [11]. Additionally, this technology fulfills other specific requirements for the present case as the significant reduction in manufacturing costs.…”

Section: B Electrode Design and Fabricationmentioning

confidence: 99%

Automatic system for electroporation of adherent cells growing in standard multi-well plates

García-Sánchez

Guitart

Rosell

et al. 2012

2012 Annual International Conference of the IEEE Engineering in Medicine and Biology Society

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Abstract-In this study an automatic system is presented to perform electroporation, also known as electropermeabilization, on adherent cells. It is an intention of this system to apply electric field pulses directly to cells growing in standard multiwell plates as a step forward to include this technique in standard laboratory protocols. An interdigitated microelectrode assembly constructed with Printed Circuit Board (PCB) is placed closely above the cell monolayer, and in order to avoid direct contact with cells, small micro-separators were included in the structure. Additionally, distribution of current density was modified by filling the gap between adjacent electrodes with a non conductive material as predicted by electric field simulations. This modification helps to concentrate the electric field intensity in the region where cells are present. The device was tested using C2C12 cell line growing adhered in 24 multiwell plates and fluorescent labeled dextran FD20S as the molecule to be delivered. Successful transfection was observed with minimal invasiveness of the operation reducing the stress caused to cells.

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Section: B Electrode Design and Fabricationmentioning

confidence: 99%

Automatic system for electroporation of adherent cells growing in standard multi-well plates

García-Sánchez

Guitart

Rosell

et al. 2012

2012 Annual International Conference of the IEEE Engineering in Medicine and Biology Society

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“…The height of the channel, 30 μm, is determined with a transparent double-sided self-adhesive tape (Tesa 4983) which consists of Polyethylene Terephthalate (PET) backing and a modified acrylic adhesive. This tape, which is characterized as a biocompatible material, defines the sidewalls of the microfluidic channel and seals it tightly [1].…”

Section: Materials Compatibilitymentioning

confidence: 99%

“…I in is the current of the current source, R S the output resistance of the current source, C T the total input capacitance (including the input capacitance C in of the amplifier), R F is the feedback resistor and C F is a capacitor connected in parallel to the feedback resistor and includes also the stray capacitance. The noise gain (NG) transfer function for this circuit is expressed by: In order to maintain the stability, a feedback capacitor C F is placed across R F to create a pole at f P in the noise gain function (1). The noise gain's zero is defined by: The higher the values of R F and C T , the sooner the NG peaking starts and the higher the value of C T, the higher the value of the high frequency noise gain is.…”

Section: Current -To-voltage Converters and Amplifiersmentioning

confidence: 99%

Front-end electronics for impedimetric microfluidic devices

et al. 2011

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Impedance spectroscopy is a common approach in assessing passive electrical properties of biological matter, however, serious problems appear in microfluidic devices in connection with distortion free signal acquisition from microelectrodes. The quality of impedance measurements highly depends on the presence of stray capacitances, signal distortions, and accompanying noises. Measurement deficiencies may be minimized with optimized electronics and sensing electrodes. The quality can further be improved with appropriate selection of measuring signals and also with selection of measuring methods such as a choice between current or voltage sources and between differential or singleended techniques. The microfluidic device that we present here incorporates an impedance sensor, which consists of an array of two sequential pairs of parallel microelectrodes, embedded in a microfluidic channel. All electronics and fluidic components are placed inside a metal holder, which ensures electric and fluidic connections to peripheral instruments. This configuration provides short electric connections and proper shielding. The method that we are using to evaluate the sample's impedance is the differential measurement technique, capable of suppressing the common mode signals and interferences, appearing in the signal-conditioning front-end circuit. Besides, it opens the possibility for compensating stray effects of the electrodes. For excitation we employ wideband signals, such as chirps or multifreqyency signals, which allow fast measurements, essential in the most impedimetric experiments in biology. The impedance spectra cover the frequency range between 10kHz -10MHz. This is essential for accessing information relating to β-dispersion, which characterizes the cell's structural properties. We present two measurement schemes: (i) an in-phase differential method, which employs two transimpedance amplifiers, and (ii) an anti-phase method, which uses one transimpedance amplifier. In this study we analyze and compare the sensitivity, signal-to-noise-ratio, and operational bandwidths of these two methods against other commonly used related circuits.

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“…for circuit paths, ber reinforced epoxy binders, polyimide and polyurethane foils for dielectrics and various glues. Only a few of them are biocompatible, as shown in tests with hippocampus cells by Mazzuferi et al [1]. Thus, dense packaging for any in vivo use is essential to prevent permeation/diusion of body uids into the electronic components.…”

Section: Introductionmentioning

confidence: 99%

Study of Gas Permeation Through Thin ta-C:H Films

2015

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Protective ultra-thin barrier lms gather increasing economic interest for controlling permeation and diusion from the biological surrounding in implanted sensor and electronic devices in future medicine. Thus, the aim of this work was the investigation of the lm thickness inuence on the gas permeation barrier of ultra-thin, cytocompatible tetrahedral amorphous carbon (ta-C:H) lms on polyimide (PI) foils. Plasma-activated chemical vapor deposition (direct deposition from an ion source) was applied to deposit these diamond-like carbon lms. The results indicate high barrier to hydrogen gas permeation by all lm thicknesses (<0.2% H2 permeation compared to uncoated PI). While the thickness of the ta-C:H layers has minor inuence, the number of layers, realized by one-or double-side deposition strongly impacts the barrier eect. Finally, tests under tensile stresses showed minor impact in the elasto-plastic deformation regime, but the expected strong increase of gas permeation after exceeding the tensile strength and lm fracture.

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The biocompatibility of materials used in printed circuit board technologies with respect to primary neuronal and K562 cells

Cited by 16 publications

References 30 publications

Automatic system for electroporation of adherent cells growing in standard multi-well plates

Automatic system for electroporation of adherent cells growing in standard multi-well plates

Front-end electronics for impedimetric microfluidic devices

Study of Gas Permeation Through Thin ta-C:H Films

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