The binding of magnesium ions to DNA

Fishman, Myer M.; Isaac, J.; Schwartz, Susan T.; Stein, Samuel

doi:10.1016/0006-291x(67)90466-4

Cited by 8 publications

(3 citation statements)

References 8 publications

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“…Fishman et al (1967) have reported a similar spectral shift for E. coli DNA denatured in MgCh solutions. Their interpretation of the shift is based upon a postulated interaction between the purine and pyrimidine nitrogens and h i b i t e d .…”

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confidence: 56%

DNA melting transition in aqueous magnesium salt solutions

Ott¹,

Ziegler²,

Bauer³

1975

Biochemistry

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The melting transition of the magnesium salt of DNA has been systematically examined in the presence of various types of anions. The addition of ClO4- to a concentration of 3.0 N results in the biphasic optical transition, with the first phase exhibiting rapid reversibility and independence of the DNA concentration. This subtransition, which is interpreted as an intramolecular condensation to a collapsed form of DNA, is followed by a DNA concentration-dependent aggregation reaction. The aggregation can be reversed by increasing the ClO4- concentration to 6.0 N while elevating the temperature to post-transition levels. Alternatively, both the collapse and the aggregation can be prevented by melting in the presence of trichloroacetate, the most strongly chaotropic solvent for DNA which has been reported (K. Hamaguchi and E. P. Geiduschek (1962), J. Am. Chem. Soc. 84, 1329). The forces responsible for mediating both the collapse and the aggregation are superficially similar to those involved in maintaining duplex stability. The collapsed form, in particular, possibly possesses features in common with the condensed structures which can be produced in aqueous solution of certain polymers, such as polyethylene glycol (Lerman, L.S. (1971), Proc. Natl. Acad. Sci. U.S.A. 68, 1886).

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mentioning

confidence: 56%

DNA melting transition in aqueous magnesium salt solutions

Ott¹,

Ziegler²,

Bauer³

1975

Biochemistry

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“…However, if the DNA is denatured in the presence of Mg2+, the spectra lead to the conclusion that Mg2+ then binds also to the bases. 100 All other evidence indicates that Mg2+ binds primarily to the phosphate groups of DNA. The melting temperature (Tm) of DNA is raised from 63 to 80°in the presence of Mg2+ indicating that the double helix is stabilized by Mg2+ presumably through phosphate binding.55, 95 Upon subsequent cooling the Mg2+ is believed to hold the single chains of DNA in close proximity, thereby permitting the hydrogen bonds broken by heating to re-form.…”

Section: Alkaline Earth Metal Ionsmentioning

confidence: 99%

Sites and thermodynamic quantities associated with proton and metal ion interaction with ribonucleic acid, deoxyribonucleic acid, and their constituent bases, nucleosides, and and nucleotides

Izatt¹,

Christensen²,

Rytting³

1971

Chem. Rev.

756

415

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“…Yatsimirskii and Kris (1966) concluded that Fe3+, Cu2+, and Mn2+ attached chiefly to the phosphate group in DNA; however Cu2+ and Fe3+ also attached to DNA bases. Fishman et al (1967) concluded that the magnesium ion bound only to the phosphate group in DNA. Coates et al (1965) found cupric ion combining with heat-denatured DNA, thus suggesting that a copper complex was formed with some of the bases.…”

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confidence: 99%