The binding of cortisol by plasma protein

Upton, G. Virginia; Bondy, Philip K.

doi:10.1016/0003-9861(58)90328-x

Cited by 25 publications

(10 citation statements)

References 16 publications

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“…The * THE = tetrahydrocortisone, THA = tetrahydro-11-dehydrocorticosterone, THF = tetrahydrocortisol, THB = tetrahydrocorticosterone, Allo-THE = allotetrahydrocortisone, Allo-THA = allotetrahydro-11-dehydrocorticosterone, Allo-THF = allotetrahydrocortisol, Allo-THB = allotetrahydrocorticosterone. t ACTH denotes continuous intravenous infusion of corticotropin (4 units/hr) for 36 hours. The assays were made on the urine collected from the twelfth to the thirty-sixth hour.…”

Section: Resultsmentioning

confidence: 99%

“…It seems likely that differences in the fraction of nonprotein-bound and protein-bound steroid at physiological (trace) and pharmacological levels may explain these discrepancies. On the basis of the recent reports on the binding of cortisol (32)(33)(34)(35)(36) and corticosterone (33)(34)(35)37) to plasma proteins, it is evident that when a trace dose of the steroid is infused, close to 100 per cent of the circulating plasma steroid is protein-bound, whereas with pharmacological doses only 70 to 80 per cent is protein-bound. After the infusion of 20 to 200 mg of steroid, plasma steroid levels may range from 50 to 200 -g per 100 ml.…”

Section: Mg)mentioning

confidence: 99%

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The Metabolism of Corticosterone in Man

Peterson¹,

Pierce²

1960

J. Clin. Invest.

105

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Section: Resultsmentioning

confidence: 99%

Section: Mg)mentioning

confidence: 99%

The Metabolism of Corticosterone in Man

Peterson¹,

Pierce²

1960

J. Clin. Invest.

105

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“…6 A transfer rate constant (9) for cortisol was not derived, because almost all of the data were obtained after a single, rapid intravenous injection of cortisol rather than with maintenance of a constant plasma level. This experimental design was chosen primarily to obtain information regarding the disappearance of cortisol from 6The data are comparable with those obtained by Upton and Bondy who studied cortisol binding to plasma protein in human subjects (32). Over a wide range of high concentrations of cortisol in human plasma, these workers found that about 15% of the steroid was ultracentrifugable, i.e., that 85%o was tightly bound to plasma protein (presumably to the globulin fraction), and 15% was bound only loosely (perhaps to albumin) (10,32).…”

Section: Entry Into Csf Of Tetrahydrocortisone Anmentioning

confidence: 98%

Studies of the Blood-Cerebrospinal Fluid Barrier to Cortisol in the Dog*

Christy¹,

Fishman²

1961

J. Clin. Invest.

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This study was undertaken to investigate the entry of administered cortisol and one of its metabolic products into the cerebrospinal fluid (CSF) of dogs under a variety of conditions. Cortisol,' one of the principal circulating adrenal cortical steroids of man (1, 2) enters the CSF (3-5), but little is known about the rate at which it passes into the CSF or what factors regulate its passage. Although the relationship of CSF to the extracellular fluid of the brain is complex, it is generally accepted that the two compartments are in equilibrium, and that the blood-CSF and blood-brain barriers do not differ qualitatively in their respective permeability characteristics (6, 7). The data of this study on the entry of cortisol into CSF can therefore be considered relevant to the problem of penetration of cortisol into the brain. This is of some clinical importance with respect to the known effects of cortisol upon brain metabolism; e.g., upon seizure threshold and emotional state (8).There are abundant data which show that the rate of entry of many substances into the CSF is determined by certain of their physical and chemical properties. These properties are solubility in lipid solvents, binding to plasma protein, molecular size, and in the case of salts of weak acids and bases, pK (6, 7, 9). Since neutral steroids have no pK, and molecular size is not an important determinant of rate of entry into the CSF except with substances of very high molecular weight (6. 7), only lipid solubility and binding to plasma

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“…im Gleichgewicht mit der geringen Konzentration an freiem Oortisol im Blut [87]. Nur dieses kann durch glomeruläre Filtration ausgesohieden werden [2,10,67,79] und wahrscheinlich ist nur das freie Oortisol dem enzymatischen Abbau in der Leber zugänglioh (1,65,66]. Vermutlich reagiert auoh der negative feed-baok·Meohanismus der Rege.…”

Section: Originalienunclassified

“…nen 'und freien Anteil von Oortisol gemeinsam. Zur Bestimmung des proteingebundenen Oortisols im Plasma oder Serum wurden verschiedene Methoden angegeben: Gleichgewichtsdialyse [7,10,75], Ultrafiltration [8,79] sowie Ultrazentrifugation [3], welche sowohl die Bindung an Albumin wie an OBG erfassen. Auch die Erythrocytenaufnahme von radioaktiv markiertem Oortisol kann zu einer indirekten Messung der Proteinbindung benutzt werden [21].…”

Section: Originalienunclassified