The B-group vitamins in the red alga Palmaria palmatа (Barents Sea): Composition, seasonal changes and influence of abiotic factors

Рыжик, И. В.; Klindukh, M. P.; Dobychina, Ekaterina

doi:10.1016/j.algal.2021.102473

Cited by 8 publications

(2 citation statements)

References 28 publications

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“…After that, the supernatants were placed in Amicon Ultra-15 centrifuge concentrators (Merck, Darmstadt, Germany) with a 3 kDa pore diameter cellulose membrane and centrifuged for 15 min at 7500 rpm for complete purification. Separation, identification, and determination of the concentrations of water-soluble vitamins thiamine (B1), riboflavin (B2), pantothenic acid (B3), pyridoxine (B6), and folic acid (Bc) were carried out by capillary zone electrophoresis (CSE), and that of the vitamin nicotinamide (B5) by micellar electrokinetic chromatography (MEKC) according to the practical guidelines for the use of "Kapel" capillary electrophoresis systems [30]. Detection conditions for vitamins B1, B2, B3, B6, and Bc: capillary: quartz, 50 microns, 75 cm; voltage + 25 kV; wavelength at the beginning of the analysis 200 nm, after the detection peak B1 267 nm, after the peak B2 and before the end of the analysis 200 nm; temperature 30 • C; pressure at the beginning of the analysis 0 mbar, after the peak B6-30 mbar until the end of the analysis.…”

Section: Determination Of Water-soluble B Vitaminsmentioning

confidence: 99%

The Combination of In Vitro Assessment of Stress Tolerance Ability, Autoaggregation, and Vitamin B-Producing Ability for New Probiotic Strain Introduction

et al. 2022

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The health benefits of probiotics are beyond doubt. The positive effects of lactobacilli and bifidobacteria on the function of many body systems have been repeatedly proven by various studies. To completely realize the potential of probiotic microorganisms, the strains should be tested by the greatest combination of characteristics that contribute to the wellness of the host. In this work, for the first time, a combined assessment of the probiotic properties and vitamin B-producing potential of various species and strains of bifidobacteria and lactobacilli was carried out. The presence of an additional advantage, such as vitamin-producing ability, can prevent vitamin deficiency both at the level of the consumption of fermented foods, when the enrichment will occur naturally on the spot, and during colonization by these intestinal strains, when synthesis will occur in vivo. To select potential probiotics, the stress tolerance ability of 16 lactic acid bacteria and bifidobacteria strains to low pH values, bile, and proteolytic enzymes, as well as their ability to autoaggregate, were studied under conditions of modeling the gastrointestinal tract in vitro. The ability of the strains to extracellularly accumulate water-soluble B vitamins was evaluated by capillary electrophoresis. Among the tested strains of bifidobacteria, B. adolescentis VKPM AC-1662 is of interest; it was characterized by the greatest stress tolerance ability and the ability to autoaggregate, in addition to the extracellular synthesis of riboflavin and pyridoxine. Among lactic acid bacteria, L. sakei VKPM B-8936 demonstrated the greatest tolerance to low pH, L. plantarum VKPM B–11007 to duodenal conditions, L. acidophilus VKPM B-2213 to pepsin, and L. salivarius VKPM B–2214 to pancreatin. The highest percentage of autoaggregation was observed in L. salivarius VKPM B-2214, which also accumulated the largest amount of pantothenic acid, but it was sensitive to stress conditions. The obtained results could be used to create new products enriched with probiotics and B vitamins.

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Section: Determination Of Water-soluble B Vitaminsmentioning

confidence: 99%

The Combination of In Vitro Assessment of Stress Tolerance Ability, Autoaggregation, and Vitamin B-Producing Ability for New Probiotic Strain Introduction

et al. 2022

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“…Free amino acid (FAA) content was determined by the capillary electrophoresis method with preliminary derivatization of amino acids using phenylisothiocyanate (PITC) ( Ryzhik et al., 2021 ). The measurements were taken three times and the values averaged.…”

Section: Methodsmentioning

confidence: 99%