“…By contrast, 5S rDNA signals were revealed in 2, 3, and 5 pairs of chromosomes in H. nemurus, H. wyckii, and H. filamentus, respectively. Multiple loci of minor ribosomal genes have been found in some fishes (Martins and Galetti, 2001;Hatanaka and Galetti, 2004;Rocco et al, 2005); variation in the number of 5S rDNA loci has also been observed among other species, such as the red wolf fish Erythrinus erythrinus, which has more than 20 sites (Cioffi et al, 2010), the cichlid Astatotilapia latifasciata, which has 15 clusters (Poleto et al, 2010) and the cichlid Laetacara dorsigera, which has 14 sites (Nakajima et al, 2012), In general, 5S rDNA loci can be found in the interstitial regions of fish chromosomes, and their pattern may represent an ancestral condition or even some advantage for the genome organization of these sequences (Martins and Galetti, 2001). H. wyckioides, M. atrifasciatus, and M. multiradiatus appear to maintain the ancestral location of the 5S rDNA loci, while the other genera studied had 5S rDNA loci at the terminal positions of the chromosomes.…”