1989
DOI: 10.1128/jvi.63.12.5319-5327.1989
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Abstract: The purified integration protein (IN) of avian myeloblastosis virus is shown to nick double-stranded oligodeoxynucleotide substrates that mimic the ends of the linear form of viral DNA. In the presence of Mg2+, nicks are created 2 nucleotides from the 3' OH ends of both the U5 plus strand and the U3 minus strand. Similar cleavage is observed in the presence of Mn2' but only when the extent of the reaction is limited. Neither

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Cited by 305 publications
(215 citation statements)
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References 37 publications
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“…Independent studies by Murphy and Goff (16) with murine leukemia virus support a role for U5-IR sequences in reverse transcription. In Rous sarcoma virus (RSV) DNA, the U5-IR stem falls within U5 sequences that have been shown to be recognized and utilized by IN for integration (11). Thus, mutations that disrupt the U5-IR stem structure and alter its nucleotide sequence cause defects in integration as well as reverse transcription (7).…”
mentioning
confidence: 99%
“…Independent studies by Murphy and Goff (16) with murine leukemia virus support a role for U5-IR sequences in reverse transcription. In Rous sarcoma virus (RSV) DNA, the U5-IR stem falls within U5 sequences that have been shown to be recognized and utilized by IN for integration (11). Thus, mutations that disrupt the U5-IR stem structure and alter its nucleotide sequence cause defects in integration as well as reverse transcription (7).…”
mentioning
confidence: 99%
“…Initial methods used radioactively labeled DNA oligonucleotides comprising the terminal cis-acting sequences of linear viral DNA required for integration. The joining of the processed strand to the other strand (self-integration) or to supplemented target DNAs can be analyzed by PAGE (Katz et al, 1990;Katzman et al, 1989). A less time-consuming, non-radioactive method involves timeresolved fluorescence anisotropy measurement using a 21-meric oligonucleotide fluorescently labeled on the terminal GT dinucleotide.…”
Section: Integrasementioning
confidence: 99%
“…Mn2+ has been observed to relax sequence specificity in other phosphoryl transfer reactions including those mediated by ASLV (Katzman et al, 1989) and HIV-1 (Drelich et al, 1992) integrase proteins and restriction endonucleases EcoRI (Hsu and Berg, 1978) and EcoRV (Vermote and Halford, 1992). For EcoRI and EcoRV, there is strong evidence suggesting that sequence-specific binding induces a conformational change in the enzymesubstrate complex which is required for catalysis.…”
Section: Effects Of Mn2+ On Tn 10 Transpositionmentioning
confidence: 99%