The avian pathogenic Escherichia coli O2 strain E058 carrying the defined aerobactin-defective iucD or iucDiutA mutation is less virulent in the chicken

Gao, Qingqing; Jia, Xingxing; Wang, Xiaobo; Xiong, Liping; Gao, Song; Liu, Xiufan

doi:10.1016/j.meegid.2014.12.038

Cited by 12 publications

(4 citation statements)

References 47 publications

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“…Similar findings were observed in a chicken infection model. An aerobactin-defective mutant of iutA was constructed and showed significantly decreased pathogenicity compared with the wild-type strain, as evidenced by the low extent of colonization in selected organs or being outcompeted in vivo ( Gao et al, 2015 ). Moreover, a case–control clinical study found that iutA was an independent risk factor associated with 30-day mortality in patients with KP BSI.…”

Section: Discussionmentioning

confidence: 99%

Clinical and Bacterial Characteristics of Klebsiella pneumoniae Affecting 30-Day Mortality in Patients With Bloodstream Infection

Shi

Shen

et al. 2021

Front. Cell. Infect. Microbiol.

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BackgroundThere is a paucity of studies using clinical characteristics and whole-genome sequencing together to fully identify the risk factors of patients with Klebsiella pneumoniae (KP) bloodstream infection (BSI).MethodsWe retrospectively analyzed the clinical and microbiological characteristics of patients with KP BSI. Isolates were processed using Illumina NGS, and relevant bioinformatics analysis was conducted (multi-locus sequence typing, serotype, phylogenetic reconstruction, detection of antibiotic resistance, and virulence genes). A logistic regression model was used to evaluate the risk factors of hosts and causative KP isolates associated with 30-day mortality in patients infected with KP BSI.ResultsOf the 79 eligible patients, the 30-day mortality rate of patients with KP BSI was 30.4%. Multivariate analysis showed that host-associated factors (increased APACHE II score and septic shock) were strongly associated with increased 30-day mortality. For the pathogenic factors, carriage of iutA (OR, 1.46; 95% CI, 1.11–1.81, p = 0.002) or Kvar_1549 (OR, 1.31; 95% CI, 1.02–1.69, p = 0.043) was an independent risk factor, especially when accompanied by a multidrug-resistant phenotype. In addition, ST11-K64 hypervirulent carbapenem-resistant KP co-harbored acquired blaKPC-2 together with iutA (76.5%, 13/17) and Kvar_1549 (100%, 17/17) genes. Comparative genomic analysis showed that they were clustered together based on a phylogenetic tree, and more virulence genes were observed in the group of ST11-K64 strains compared with ST11-non-K64. The patients infected with ST11-K64 strains were associated with relatively high mortality (47.2%, 7/17).ConclusionThe carriage of iutA and Kvar_1549 was seen to be an independent mortality risk factor in patients with KP BSI. The identification of hypervirulent and carbapenem-resistant KP strains associated with high mortality should prompt surveillance.

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Section: Discussionmentioning

confidence: 99%

Clinical and Bacterial Characteristics of Klebsiella pneumoniae Affecting 30-Day Mortality in Patients With Bloodstream Infection

Shi

Shen

et al. 2021

Front. Cell. Infect. Microbiol.

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“…Several virulence factors have been associated with APEC strains [ 408 ]. These include fimbrial adhesins (type 1 fimbriae [ 409 ], P fimbriae [ 410 ], and Stg fimbriae [ 411 ], curli [ 409 ], Yqi [ 412 ]; outer membrane proteins and other surface molecules contributing to serum resistance or antiphagocytic properties (Increased serum survival (Iss) [ 413 , 414 ]), two-component signal transduction systems (RstA/RstB [ 415 ], PhoB/PhoR [ 416 ], BarA/UvrY [ 417 ]), O78 lipopolysaccharide, and K1 capsule [ 418 , 419 ]; iron and metal acquisition systems (aerobactin [ 420 ], salmochelin [ 421 ], yersiniabactin [ 422 ]), heme utilization/transport protein ChuA [ 423 ] and the Sit metal transport system [ 424 ]; autotransporters (Tsh, Vat, Sha, TagB, TagC, AatA [ 425 , 426 , 427 , 428 ], phosphate transport and the Pho regulon (the Pst system) [ 429 ]; sugar metabolism [ 430 ]; and nitrite transporter NirC [ 431 ]. Specific virulence genes including iss , iroN , ompT, iutA and hlyF are commonly present in APEC and are frequently encoded on large plasmids such as Colicin V (ColV) plasmids [ 432 ].…”

Section: Pathogenic E Coli Of Importance To Animal...mentioning

confidence: 99%

The Diversity of Escherichia coli Pathotypes and Vaccination Strategies against This Versatile Bacterial Pathogen

2023

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Escherichia coli (E. coli) is a gram-negative bacillus and resident of the normal intestinal microbiota. However, some E. coli strains can cause diseases in humans, other mammals and birds ranging from intestinal infections, for example, diarrhea and dysentery, to extraintestinal infections, such as urinary tract infections, respiratory tract infections, meningitis, and sepsis. In terms of morbidity and mortality, pathogenic E. coli has a great impact on public health, with an economic cost of several billion dollars annually worldwide. Antibiotics are not usually used as first-line treatment for diarrheal illness caused by E. coli and in the case of bloody diarrhea, antibiotics are avoided due to the increased risk of hemolytic uremic syndrome. On the other hand, extraintestinal infections are treated with various antibiotics depending on the site of infection and susceptibility testing. Several alarming papers concerning the rising antibiotic resistance rates in E. coli strains have been published. The silent pandemic of multidrug-resistant bacteria including pathogenic E. coli that have become more difficult to treat favored prophylactic approaches such as E. coli vaccines. This review provides an overview of the pathogenesis of different pathotypes of E. coli, the virulence factors involved and updates on the major aspects of vaccine development against different E. coli pathotypes.

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“…It was present in our and in two previous schemes (Johnson et al, 2008;Schouler et al, 2012), and is part of the five genes of the aerobactin operon. Gao et al (2015) showed that aerobactin-defective mutants of an O2 E. coli have significantly decreased pathogenicity in challenged chickens. Another study detected the iutA and the iroN genes in respectively 91.9% and 79.9% of the strains isolated from colibacillosis lesions from 60 commercial broiler farms in Korea (Kim et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

Description and validation of a new set of PCR markers predictive of avian pathogenic Escherichia coli virulence

Lucas

Delannoy

Schouler

et al. 2022

Veterinary Microbiology

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The avian pathogenic Escherichia coli O2 strain E058 carrying the defined aerobactin-defective iucD or iucDiutA mutation is less virulent in the chicken

Cited by 12 publications

References 47 publications

Clinical and Bacterial Characteristics of Klebsiella pneumoniae Affecting 30-Day Mortality in Patients With Bloodstream Infection

Clinical and Bacterial Characteristics of Klebsiella pneumoniae Affecting 30-Day Mortality in Patients With Bloodstream Infection

The Diversity of Escherichia coli Pathotypes and Vaccination Strategies against This Versatile Bacterial Pathogen

Description and validation of a new set of PCR markers predictive of avian pathogenic Escherichia coli virulence

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