The atlas of mouse development eHistology resource

Graham, Elizabeth; Moss, Julie; Burton, Nick; Roochun, Yogmatee; Armit, Chris; Richardson, Lorna; Baldock, Richard

doi:10.1242/dev.128181

Cited by 11 publications

(14 citation statements)

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“…(h) Schematic showing a parasagittal view through an E16.5 mouse head, with dotted lines showing the approximate location of the most rostral and most caudal sections shown in (a–g). (i,j) Low‐power (i) and high‐power (j) views of a hematoxylin and eosin‐stained parasagittal section through an E16.5 mouse head (Plate 38c, image a, from the eHistology Atlas with Kaufman annotations; Graham et al, ). The dotted lines in (j) show the approximate locations of the sections shown in (a–g).…”

Section: Resultsmentioning

confidence: 99%

Olfactory ensheathing cells abutting the embryonic olfactory bulb express Frzb, whose deletion disrupts olfactory axon targeting

Rich

Perera

Andratschke

et al. 2018

Glia

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We and others previously showed that in mouse embryos lacking the transcription factor Sox10, olfactory ensheathing cell (OEC) differentiation is disrupted, resulting in defective olfactory axon targeting and fewer gonadotropin‐releasing hormone (GnRH) neurons entering the embryonic forebrain. The underlying mechanisms are unclear. Here, we report that OECs in the olfactory nerve layer express Frzb —encoding a secreted Wnt inhibitor with roles in axon targeting and basement membrane breakdown—from embryonic day (E)12.5, when GnRH neurons first enter the forebrain, until E16.5, the latest stage examined. The highest levels of Frzb expression are seen in OECs in the inner olfactory nerve layer, abutting the embryonic olfactory bulb. We find that Sox10 is required for Frzb expression in OECs, suggesting that loss of Frzb could explain the olfactory axon targeting and/or GnRH neuron migration defects seen in Sox10 ‐null mice. At E16.5, Frzb ‐null embryos show significant reductions in both the volume of the olfactory nerve layer expressing the maturation marker Omp and the number of Omp‐positive olfactory receptor neurons in the olfactory epithelium. As Omp upregulation correlates with synapse formation, this suggests that Frzb deletion indeed disrupts olfactory axon targeting. In contrast, GnRH neuron entry into the forebrain is not significantly affected. Hence, loss of Frzb may contribute to the olfactory axon targeting phenotype, but not the GnRH neuron phenotype, of Sox10 ‐null mice. Overall, our results suggest that Frzb secreted from OECs in the olfactory nerve layer is important for olfactory axon targeting.

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Section: Resultsmentioning

confidence: 99%

Olfactory ensheathing cells abutting the embryonic olfactory bulb express Frzb, whose deletion disrupts olfactory axon targeting

Rich

Perera

Andratschke

et al. 2018

Glia

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“…Extensions from the brain (cranial nerves [CN]) and spinal cord (peripheral nerves) reach the periphery, often after passing through one or two ganglia. Structural relationships among brain and spinal cord domains in space and over time may be explored in more detail using developmental neuroanatomy atlases (eHistology Atlas [emouseatlas.org, retrieved February, 2017], (Graham et al 2015; Jacobowitz and Abbot, 1998; Kaufman, 1992; Paxinos et al 2007; Schambra, 2008; Theiler, 1989). …”

Section: Overview Of Early Cns Development (E70–e105)mentioning

confidence: 99%

Histology Atlas of the Developing Prenatal and Postnatal Mouse Central Nervous System, with Emphasis on Prenatal Days E7.5 to E18.5

et al. 2017

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Evaluation of the central nervous system (CNS) in the developing mouse presents unique challenges given the complexity of ontogenesis, marked structural reorganization over very short distances in three dimensions each hour, and numerous developmental events susceptible to genetic and environmental influences. Developmental defects affecting the brain and spinal cord arise frequently both in utero and perinatally as spontaneous events, following teratogen exposure, and as sequelae to induced mutations, and thus are a common factor in embryonic and perinatal lethality in many mouse models. Knowledge of normal organ and cellular architecture and differentiation throughout the mouse’s lifespan is crucial to identify and characterize neurodevelopmental lesions. By providing a well-illustrated overview summarizing major events of normal in utero and perinatal mouse CNS development with examples of common developmental abnormalities, this annotated, color atlas can be used to identify normal structure and histology when phenotyping genetically engineered mice (GEM) and will enhance efforts to describe and interpret brain and spinal cord malformations as causes of mouse embryonic and perinatal lethal phenotypes. The schematics and images in this atlas illustrate major developmental events during gestation from embryonic day (E) 7.5 to E18.5 and after birth from postnatal day (P)1 to P21.

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“…The eHistology resource provides online access to cellular-resolution images of the histology sections used in Kaufman's The Atlas of Mouse Development (Graham et al, 2015). The original publication of this book by Kaufman (1992) is the definitive work for mouse developmental anatomy.…”

Section: Ehistology and Mouse Embryo Phenotypingmentioning

confidence: 99%

“…This is critical as gene expression patterns do not necessarily correspond to named anatomical structures and can be difficult to describe using only text-based annotation. Recently, we have developed and made freely available the eHistology resource (Graham et al, 2015) as a means of capturing cellular-resolution anatomical and histological detail. This resource utilises the sections and annotations originally used in the ‘The Atlas of Mouse Development’ (Kaufman 1994), and makes this data available through a web-accessible pan-and-zoom interface.…”

Section: Introductionmentioning

confidence: 99%

eMouseAtlas: An atlas-based resource for understanding mammalian embryogenesis

Armit

Richardson

Venkataraman

et al. 2017

Developmental Biology

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The eMouseAtlas resource is an online database of 3D digital models of mouse development, an ontology of mouse embryo anatomy and a gene-expression database with about 30K spatially mapped gene-expression patterns. It is closely linked with the MGI/GXD database at the Jackson Laboratory and holds links to almost all available image-based gene-expression data for the mouse embryo. In this resource article we describe the novel web-based tools we have developed for 3D visualisation of embryo anatomy and gene expression. We show how mapping of gene expression data onto spatial models delivers a framework for capturing gene expression that enhances our understanding of development, and we review the exploratory tools utilised by the EMAGE gene expression database as a means of defining co-expression of in situ hybridisation, immunohistochemistry, and lacZ-omic expression patterns. We report on recent developments of the eHistology atlas and our use of web-services to support embedding of the online ‘The Atlas of Mouse Development’ in the context of other resources such as the DMDD mouse phenotype database. In addition, we discuss new developments including a cellular-resolution placental atlas, third-party atlas models, clonal analysis data and a new interactive eLearning resource for developmental processes.

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The atlas of mouse development eHistology resource

Cited by 11 publications

References 0 publications

Olfactory ensheathing cells abutting the embryonic olfactory bulb express Frzb, whose deletion disrupts olfactory axon targeting

Olfactory ensheathing cells abutting the embryonic olfactory bulb express Frzb, whose deletion disrupts olfactory axon targeting

Histology Atlas of the Developing Prenatal and Postnatal Mouse Central Nervous System, with Emphasis on Prenatal Days E7.5 to E18.5

eMouseAtlas: An atlas-based resource for understanding mammalian embryogenesis

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