The association of CAPN1 316 marker genotypes with growth and meat quality traits of steers finished on pasture

Abstract

The objective of this paper was to determine the association of a SNP in the μ-calpain gene at position 316 with growth and quality of meat traits of steers grown on pasture. Fifty-nine Brangus and 20 Angus steers were genotyped for CAPN1 316. Warner Bratzler shear force was measured in l. lumborum samples after a 7-day aging period. A multivariate analysis of variance was performed, including shear force (WBSF), final weight (FW), average daily gain (ADG), backfat thickness (BFT), average monthly fat thickness gain (AMFTG), rib-eye area (REA), and beef rib-eye depth (RED) as dependent variables. The CAPN1 316 genotype was statistically significant. Univariate analyses were done with these variables. The marker genotype was statistically significant (p < 0.05) for WBSF (kg: CC: 4.41 ± 0.57; CG: 5.58 ± 0.20; GG: 6.29 ± 0.18), FW (kg: CC: 360.23 ± 14.71; CG: 381.34 ± 5.26; GG: 399.23 ± 4.68), and ADG (kg/d: CC: 0.675 ± 0.046; CG: 0.705 ± 0.016; GG: 0.765 ± 0.014) Shear force, final weight and average daily gain were significantly different according to the CAPN1 316 marker genotypes. The marker genotype was statistically significant in the multivariate analysis (p = 0.001). The first characteristic root explained 89% of the differences among genotypes. WBSF, FW and ADG were the most important traits in the first vector, indicating that animals with the marker genotype for lowest WBSF also have the lowest FW and ADG.

Individual and combined effects of &lt;i&gt;CAPN1&lt;/i&gt;, &lt;i&gt;CAST&lt;/i&gt;, &lt;i&gt;LEP&lt;/i&gt; and &lt;i&gt;GHR&lt;/i&gt; gene polymorphisms on carcass characteristics and meat quality in Holstein bulls

“…Animals with the GG genotype had +52.7 kg heavier LW and +34.4 kg heavier HCW compared to the CC genotype in the present study. In the literature, CAPN1 G316A was evaluated as an effective marker on beef tenderness in several studies indicating that the C allele is associated with more tender meat (Casas et al, 2005;Corva et al, 2007;Gill et al, 2009;Miquel et al, 2009;Smith et al, 2009;Curi et al, 2010). It has been shown that, along with loci affecting beef tenderness, other loci associated with weaning weight and carcass weights were mapped to bovine chromosome 29 (Casas et al, 2005).…”

“…It has been shown that, along with loci affecting beef tenderness, other loci associated with weaning weight and carcass weights were mapped to bovine chromosome 29 (Casas et al, 2005). Miquel et al (2009) reported that final weight and average daily gain differentiated between the CAPN1 G316A marker genotypes and that choosing animals with the favourable marker genotype (CC) for tenderness resulted in a selection of animals with lower average daily gain and final weight in Angus and Brangus steers. In addition, Pintos and Corva (2011) found significant associations between the same marker with birth weight, weaning weight and live weight recorded at 18 months of age in Angus cattle.…”

“…In this context, one of the most investigated genes is bovine micromolar calcium-activated neutral protease 1 (CAPN1), which is located on chromosome 29, encoding the large subunit of the enzyme µ-calpain involved in degrading myofibrillar proteins under postmortem conditions. CAPN1 was suggested as a genetic factor influencing the postmortem tenderization process (Koohmaraie, 1996;Page et al, 2002;Miquel et al, 2009). The SNP G316A in exon 9 (alleles C/G) of the CAPN1 gene has been associated with meat quality traits (Gill et al, 2009;Miquel et al, 2009;Smith et al, 2009;Bonilla et al, 2010;Mazzucco et al, 2010;Pinto et al, 2010;Kaneda et al, 2011), final weight and average daily weight gain (Miquel et al, 2009;Tait et al, 2014).…”

“…CAPN1 was suggested as a genetic factor influencing the postmortem tenderization process (Koohmaraie, 1996;Page et al, 2002;Miquel et al, 2009). The SNP G316A in exon 9 (alleles C/G) of the CAPN1 gene has been associated with meat quality traits (Gill et al, 2009;Miquel et al, 2009;Smith et al, 2009;Bonilla et al, 2010;Mazzucco et al, 2010;Pinto et al, 2010;Kaneda et al, 2011), final weight and average daily weight gain (Miquel et al, 2009;Tait et al, 2014). The SNP V530I in exon 14 of the CAPN1 gene has been associated with beef tenderness (Corva et al, 2007;Allais et al, 2011), meat colour and drip loss (Ribeca et al, 2013).…”

Estimación de las frecuencias alélicas y genotípicas de los genes CAPN1 Y CAST asociados a la calidad de la carne en bovinos de la Cuenca del Papaloapan

“…El polimorfismo CAPN1-316 está relacionado con variaciones en las características de ganancia en peso vivo y peso a la matanza en animales de las razas Angus y Brangus criados bajo pastoreo y sacrificados a un espesor de grasa dorsal promedio de 6.0 mm (Miquel et al, 2009). 91 %, 0 %, 9 %, respectivamente.…”