The arginine deiminase pathway in the wine lactic acid bacterium Lactobacillus hilgardii X 1 B: structural and functional study of the arcABC genes

Arena, Mario Eduardo; Nadra, Marı́a C. Manca de; Múñoz, Rosario

doi:10.1016/s0378-1119(02)01083-1

Cited by 50 publications

(34 citation statements)

References 27 publications

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“…Lmo0036 represents the first example of a carbamoyltransferase which is able to catalyse reversible ornithine and putrescine carbamoyltransfer reactions OTC is widely distributed in Gram-positive and Gramnegative bacteria and comprises two subtypes, aOTC and cOTC (Arena et al, 2002;Casiano-Coló n & Marquis, 1988;Legrain & Stalon, 1976;Sainz et al, 1998). aOTC catalyses the carbamoylation of ornithine to form citrulline participating in de novo biosynthesis of arginine, while cOTC performs the decarbamoylation of citrulline to form ornithine and carbamoylphosphate, which represents the second step of the ADI pathway (Cunin et al, 1986;Legrain & Stalon, 1976) (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…Enterococcus faecalis (Llácer et al, 2007), Pseudomonas aeruginosa (Sainz et al, 1998), Streptococcus mutans (Casiano-Coló n & Marquis, 1988) and Lactobacillus hilgardii (Arena et al, 2002), and these are of critical importance for ADI and AgDI pathways involved in pH homeostasis and microbial ecology. While cOTC mediates the decarbamoylation of citrulline (the deimination product of arginine) yielding carbamoylphosphate and ornithine ( Fig.…”

Section: Introductionmentioning

confidence: 99%

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Lmo0036, an ornithine and putrescine carbamoyltransferase in Listeria monocytogenes, participates in arginine deiminase and agmatine deiminase pathways and mediates acid tolerance

et al. 2011

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Listeria monocytogenes is a foodborne pathogen causing listeriosis. Acid is one of the stresses that foodborne pathogens encounter most frequently. The ability to survive and proliferate in acidic environments is a prerequisite for infection. However, there is limited knowledge about the molecular basis of adaptation of L. monocytogenes to acid. Arginine deiminase (ADI) and agmatine deiminase (AgDI) systems are implicated in bacterial tolerance to acidic environments. Homologues of ADI and AgDI systems have been found in L. monocytogenes lineages I and II strains. Sequence analysis indicated that lmo0036 encodes a putative carbamoyltransferase containing conserved motifs and residues important for substrate binding. Lmo0036 acted as an ornithine carbamoyltransferase and putrescine carbamoyltransferase, representing the first example, to our knowledge, that catalyses reversible ornithine and putrescine carbamoyltransfer reactions. Catabolic ornithine and putrescine carbamoyltransfer reactions constitute the second step of ADI and AgDI pathways. However, the equilibrium of in vitro carbamoyltransfer reactions was overwhelmingly towards the anabolic direction, suggesting that catabolic carbamoyltransferase was probably the limiting step of the pathways. lmo0036 was induced at the transcriptional level when L. monocytogenes was subjected to low-pH stress. Its expression product in Escherichia coli exhibited higher catabolic carbamoyltransfer activities under acidic conditions. Consistently, absence of this enzyme impaired the growth of Listeria under mild acidic conditions (pH 4.8) and reduced its survival in synthetic human gastric fluid (pH 2.5), and corresponded to a loss in ammonia production, indicating that Lmo0036 was responsible for acid tolerance at both sublethal and lethal pH levels. Furthermore, Lmo0036 played a possible role in Listeria virulence.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Lmo0036, an ornithine and putrescine carbamoyltransferase in Listeria monocytogenes, participates in arginine deiminase and agmatine deiminase pathways and mediates acid tolerance

et al. 2011

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“…and Cup-Cdown amplify fragments of 0.3 and 0.5 kb, of the genes coding for OTC and 12 CK, respectively (Table 1) (Arena et al, 2002). The PCR reaction mixture and the 13 amplification conditions were described above for the amplification of the 16S rDNA.…”

Section: Presence Of Arginine Deiminase Pathway Genes 5mentioning

confidence: 99%

Efficacy of recA gene sequence analysis in the identification and discrimination of Lactobacillus hilgardii strains isolated from stuck wine fermentations

Rodríguez

Rivas

Múñoz

2007

International Journal of Food Microbiology

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“…The pathway is composed of the three key enzymes: ArcA, ArcB, and ArcC, corresponding to arginine deiminase, ornithine carbamoyl transferase, and carbamate kinase, respectively (7)(8)(9) (Fig. 1).…”

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confidence: 99%

Streptococcus pyogenes Arginine and Citrulline Catabolism Promotes Infection and Modulates Innate Immunity

2014

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A bacterium's ability to acquire nutrients from its host during infection is an essential component of pathogenesis. For the Gram-positive pathogen Streptococcus pyogenes, catabolism of the amino acid arginine via the arginine deiminase (ADI) pathway supplements energy production and provides protection against acid stress in vitro. Its expression is enhanced in murine models of infection, suggesting an important role in vivo. To gain insight into the function of the ADI pathway in pathogenesis, the virulence of mutants defective in each of its enzymes was examined. Mutants unable to use arginine (⌬ArcA) or citrulline (⌬ArcB) were attenuated for carriage in a murine model of asymptomatic mucosal colonization. However, in a murine model of inflammatory infection of cutaneous tissue, the ⌬ArcA mutant was attenuated but the ⌬ArcB mutant was hyperattenuated, revealing an unexpected tissue-specific role for citrulline metabolism in pathogenesis. When mice defective for the arginine-dependent production of nitric oxide (iNOS ؊/؊ ) were infected with the ⌬ArcA mutant, cutaneous virulence was rescued, demonstrating that the ability of S. pyogenes to utilize arginine was dispensable in the absence of nitric oxide-mediated innate immunity. This work demonstrates the importance of arginine and citrulline catabolism and suggests a novel mechanism of virulence by which S. pyogenes uses its metabolism to modulate innate immunity through depletion of an essential host nutrient.

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The arginine deiminase pathway in the wine lactic acid bacterium Lactobacillus hilgardii X 1 B: structural and functional study of the arcABC genes

Cited by 50 publications

References 27 publications

Lmo0036, an ornithine and putrescine carbamoyltransferase in Listeria monocytogenes, participates in arginine deiminase and agmatine deiminase pathways and mediates acid tolerance

Lmo0036, an ornithine and putrescine carbamoyltransferase in Listeria monocytogenes, participates in arginine deiminase and agmatine deiminase pathways and mediates acid tolerance

Efficacy of recA gene sequence analysis in the identification and discrimination of Lactobacillus hilgardii strains isolated from stuck wine fermentations

Streptococcus pyogenes Arginine and Citrulline Catabolism Promotes Infection and Modulates Innate Immunity

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