The application of pigment-protein fraction from Nannochloropsis oculata on β-actin response of Cromileptes altivelis infected with viral nervous necrosis

Yanuhar, Uun; Khumaidi, Achmad

doi:10.19027/jai.16.1.22-32

Cited by 8 publications

(10 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Proteins of Nannochloropsis oculata increase α actin activity and immunity system defense, decreasing the mortality linked to Betanodavirus (or nervous disease virus, NNV), an RNA non-enveloped virus from the Nodaviridae family causing the nerval necrosis of the humpback grouper [ 109 ].…”

Section: Microalgae Antiviral Compoundsmentioning

confidence: 99%

See 1 more Smart Citation

Evaluation of Microalgae Antiviral Activity and Their Bioactive Compounds

et al. 2021

View full text Add to dashboard Cite

During the last year, science has been focusing on the research of antivirally active compounds overall after the SARS-CoV-2 pandemic, which caused a great amount of deaths and the downfall of the economy in 2020. Photosynthetic organisms such as microalgae are known to be a reservoir of bioactive secondary metabolites; this feature, coupled with the possibility of achieving very high biomass levels without excessive energetic expenses, make microalgae worthy of attention in the search for new molecules with antiviral effects. In this work, the antiviral effects of microalgae against some common human or animal viruses were considered, focusing our attention on some possible effects against SARS-CoV-2. We summed up the data from the literature on microalgae antiviral compounds, from the most common ones, such as lectins, polysaccharides and photosynthetic pigments, to the less known ones, such as unidentified proteins. We have discussed the effects of a microalgae-based genetic engineering approach against some viral diseases. We have illustrated the potential antiviral benefits of a diet enriched in microalgae.

show abstract

Section: Microalgae Antiviral Compoundsmentioning

confidence: 99%

“…A diet rich in fatty acids of Chlorella increases the immunity system of people aged 50–55 after influenza vaccination, and of Salmon trutta caspius after infection with NNV [ 109 ].…”

Section: An Overview Of the Antiviral Effects Of A Supplementary Microalgae Diet And Its Possible Action On Sars-cov-2mentioning

confidence: 99%

Evaluation of Microalgae Antiviral Activity and Their Bioactive Compounds

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Their tests were performed by an oral method with the aid of feeding tube hose performed for three different days: 0 days, 5 th day, and 10 th day. This research conducted using a dose of 33 μg/mL, where is referred to the previous clinical test [16] that is by using protein purification C. vulgaris.…”

Section: In-vivo Test Of Crude Extract Of Cvulgaris In Cantang Groupmentioning

confidence: 99%

“…The final preparation step was washing the tissues under running water for 5 min, repeated three times. After the prepared slide was dried, it was then observed under a microscope [16].…”

Section: Detections Of Heat Shock Protein (Hsp) Using Immunohistochemmentioning

confidence: 99%

“…In some studies, it has been suggested that the protein pigments from Chlorella have many benefits. Some of the bioactive compound of them can be developed and applied as antibacterial [15] with measurable immune responses such as NF-kappa beta response [4] as well as antiviral and anti-inflammatory viral infections in the grouper [16]. The aims of this research to determine the effect of extract C. vulgaris with as an inducer of the HSP in VNN-infected Cantang grouper.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

In-Vivo Test of Chlorella vulgaris Extract for Induction into Heat Shock Proteins of Cantang Grouper (Ephinephelus fuscoguttatus-lanceolatus) Infected by Viral Nervous Necrosis

Masitha

Yanuhar

Hertika

2019

JFMR

View full text Add to dashboard Cite

Cantang Grouper fish (Ephinephelus fuscoguttatus-lanceolatus) has a high value both in international and local markets. However, the production of these fishes has decreased due to viral infection kown as VNN (Viral Nervous Necrosis). In relation to viral infections, an induced Heat Shock Protein (HSP) by Chlorella vulgaris is an effort to be used as an anti-inflammatory agent because of VNN infection. This research aimed to know the potential of C. vulgaris extracts as an inducer of heat shock protein in Cantang Grouper fish. Method used in this research was experimental method using an in-vivo test treatment with 33 μg/ml of C.vulgaris extracts in Cantang grouper fish. There were four treatments applied, they were (A) control fish, (B) fish infected with VNN, (C) fish treated with C. vulgaris extracts, and (D) fish infected by the VNN and treated with C. vulgaris extracts. Based on the phytochemical test, C. vulgaris extracts contained alkaloids, terpenoids, and tannins. Control fish showed no apparent inflammatory reaction. Fish treated with 33 μg/ml of C. vulgaris extracts had low inflammatory response of HSP70. Fish infected by VNN and treated with 33 μg/ml of C. vulgaris extract strengthened its inflammatory response as for fish infected by the VNN but without being treated by the extract showed very strong tissue inflammatory response. Results in this study indicate that that C. vulgaris extracts can inhibit the development of the virus and is capable to reduce the inflammatory response to Cantang grouper infected by VNN.

show abstract