The Application of CRISPR/Cas Systems for Antiviral Therapy

Baddeley, Helen J E; Isalan, Mark

doi:10.3389/fgeed.2021.745559

Cited by 16 publications

(11 citation statements)

References 68 publications

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“…T7E1 and SURVEYOR enzymes can cut these fragments. This cut can be detected on the agarose gel [93,94]. Another method of indel mutation detection is using a high-resolution melting curve (HRM), which can be used to identify heteroduplex DNA mismatches.…”

Section: Knock-out Detection Methodsmentioning

confidence: 99%

Principles and Applications of CRISPR Toolkit in Virus Manipulation, Diagnosis, and Virus-Host Interactions

Jamehdor

Pajouhanfar

Saba

et al. 2022

Cells

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Viruses are one of the most important concerns for human health, and overcoming viral infections is a worldwide challenge. However, researchers have been trying to manipulate viral genomes to overcome various disorders, including cancer, for vaccine development purposes. CRISPR (clustered regularly interspaced short palindromic repeats) is becoming one of the most functional and widely used tools for RNA and DNA manipulation in multiple organisms. This approach has provided an unprecedented opportunity for creating simple, inexpensive, specific, targeted, accurate, and practical manipulations of viruses, such as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), human immunodeficiency virus-1 (HIV-1), and vaccinia virus. Furthermore, this method can be used to make an effective and precise diagnosis of viral infections. Nevertheless, a valid and scientifically designed CRISPR system is critical to make more effective and accurate changes in viruses. In this review, we have focused on the best and the most effective ways to design sgRNA, gene knock-in(s), and gene knock-out(s) for virus-targeted manipulation. Furthermore, we have emphasized the application of CRISPR technology in virus diagnosis and in finding significant genes involved in virus-host interactions.

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Section: Knock-out Detection Methodsmentioning

confidence: 99%

Principles and Applications of CRISPR Toolkit in Virus Manipulation, Diagnosis, and Virus-Host Interactions

Jamehdor

Pajouhanfar

Saba

et al. 2022

Cells

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“…The Cas9-, Cas12- (both cut dsDNA), and Cas13- (cuts ssRNA) based systems have been adapted for treatment of viral infections in vitro and in vivo in mammals (Fig. 1 c) [ 23 ]. For example, EBT-101 was a first-in-human one-time gene therapy against HIV with adeno-associated virus used to deliver the CRISPR/Cas9 system [ 24 ].…”

Section: Antiviral Agents and Therapiesmentioning

confidence: 99%

Seven classes of antiviral agents

Ianevski

Ahmad

Anunnitipat

et al. 2022

Cell. Mol. Life Sci.

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The viral epidemics and pandemics have stimulated the development of known and the discovery of novel antiviral agents. About a hundred mono- and combination antiviral drugs have been already approved, whereas thousands are in development. Here, we briefly reviewed 7 classes of antiviral agents: neutralizing antibodies, neutralizing recombinant soluble human receptors, antiviral CRISPR/Cas systems, interferons, antiviral peptides, antiviral nucleic acid polymers, and antiviral small molecules. Interferons and some small molecules alone or in combinations possess broad-spectrum antiviral activity, which could be beneficial for treatment of emerging and re-emerging viral infections.

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“…However, there is an urgent need to develop new methods to combat these pathogens. Being one of the most valued molecular tools, the CRISPR-Cas system is currently being used for developing antiviral strategies in various organisms [155][156][157]. Of all nucleases described in the specialized literature, it has been shown that different variants of Cas9, Cas12, and Cas13 exhibit the most promising results regarding the antiviral approaches [155].…”

Section: Applicability Of Crispr-cas In Anti-bmnpv Therapymentioning

confidence: 99%

Advances in Editing Silkworms (Bombyx mori) Genome by Using the CRISPR-Cas System

Baci

Cucu

Giurgiu

et al. 2021

Insects

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CRISPR (clustered regularly interspaced short palindromic repeats)-Cas (CRISPR-associated) represents a powerful genome editing technology that revolutionized in a short period of time numerous natural sciences branches. Therefore, extraordinary progress was made in various fields, such as entomology or biotechnology. Bombyx mori is one of the most important insects, not only for the sericulture industry, but for numerous scientific areas. The silkworms play a key role as a model organism, but also as a bioreactor for the recombinant protein production. Nowadays, the CRISPR-Cas genome editing system is frequently used in order to perform gene analyses, to increase the resistance against certain pathogens or as an imaging tool in B. mori. Here, we provide an overview of various studies that made use of CRISPR-Cas for B. mori genome editing, with a focus on emphasizing the high applicability of this system in entomology and biological sciences.

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The Application of CRISPR/Cas Systems for Antiviral Therapy

Cited by 16 publications

References 68 publications

Principles and Applications of CRISPR Toolkit in Virus Manipulation, Diagnosis, and Virus-Host Interactions

Principles and Applications of CRISPR Toolkit in Virus Manipulation, Diagnosis, and Virus-Host Interactions

Seven classes of antiviral agents

Advances in Editing Silkworms (Bombyx mori) Genome by Using the CRISPR-Cas System

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