The antioxidant rich active principles of Clerodendrum sp. controls haloalkane xenobiotic induced hepatic damage in murine model

Abstract

Clerodendrum is a plant with potent antioxidant activity and has been frequently employed as a traditional remedy against bronchitis, asthma, liver and stomach disorders. Three species of genus Clerodendrum namely Clerodendrum indicum, C. colebrookianum and C. inerme (Syn. Volkameria inermis) were investigated for their possible activity against oxidative stress induced liver injury. Apart from generation of Reactive Oxygen Species (ROS) in the WRL-68 cell line (human hepatic cell line), in-vitro and in-vivo antioxidant assays were also assessed. Features of immune cell proliferation (MTT) were analyzed thoroughly. Gas Chromatography-Mass Spectrometry (GC–MS) and Fourier Transform Infrared Spectroscopy (FTIR) analyses have been performed to identify the active biological compounds. These active biological compounds were further subjected to molecular docking. The antioxidant activity of three Clerodendrum sp. was significantly high in DPPH, nitric oxide, hydroxyl radical and hydrogen peroxide etc. Biochemical parameters like catalase, superoxide dismutase (SOD) and reduced glutathione (GSH) were generated in excess due to CCl4 administration, which was ameliorated by treating with Clerodendrum extract. The phytochemical 24,25-Dihydroxyvitamin D shows excellent binding affinity in Autodock Vina. The present study provided convincing evidences that C. indicum and C. inerme showed good result but C. colebrookianum performed better by almost all means.

UHPLC‐ESI‐QTOF‐MS/MS Based Identification, Quantification, and Assessment of in Silico Molecular Interactions of Major Phytochemicals from Bioactive Fractions of Clerodendrum glandulosum Lindl. Leaves

“…Notably, basal ROS levels were unaffected by treatment with different fractions (100 μg/mL) of C. glandulosum extract alone ( Figure S2 in Supporting Information ). In an earlier study, C. glandulosum leaf extract showed marked protection against H 2 O 2 ‐induced oxidative insult in human hepatocyte cell lines [12] . Other studies reported that extract obtained from C. glandulosum leaf successfully restored the endogenous antioxidant status depleted during chemical as well as high‐calorie induced oxidative stress in an animal model [11,13] .…”

“…In an earlier study, C. glandulosum leaf extract showed marked protection against H 2 O 2 -induced oxidative insult in human hepatocyte cell lines. [12] Other studies reported that extract obtained from C. glandulosum leaf successfully restored the endogenous antioxidant status depleted during chemical as well as high-calorie induced oxidative stress in an animal model. [11,13] Our results corroborated with these previous reports that bioactive fractions of C. glandulosum rich in phenolic and flavonoids could inhibit ROS production effectively.…”

“…[11] Interestingly, a close correlation was found between the consumption of C. glandulosum leaves and a lower prevalence of hypertension among these tribes. [10] Extracts of C. glandulosum leaves have been reported to be promising antioxidants [12] along with exerting antidiabetic, [11] hepatoprotective, [13] and cardioprotective [11] properties during high-calorie and chemical-induced oxidative stress. [11,14,15] A few important bioactive compounds, such as verbascoside, martynoside, osmanthuside β6, oleanoleic acid, maslinic acid, and stigmasterol have been reported in C. glandulosum leaves.…”

Therapeutic assessment, chemical characterization and in-silico docking of an ethnomedicinal species, Clerodendrum serratum (Linn.) Moon

“…A total of four in-vitro free radical scavenging activity namely DPPH, hydroxyl radical, nitric oxide and hydrogen peroxide as well as phenol and flavonoid content were evaluated to study the efficacy of CSL [12].…”

Synthesis, physicochemical characterization and biological activity of synthesized Silver and Rajat Bhasma nanoparticles using Clerodendrum inerme

“…A total of eight antioxidant assays were performed by previously reported methods using silver Nano, rajatbhasma nanoparticle and plant extract (Dutta et al, 2018;Kar et al, 2019).…”