The Antioxidant and Anticancer Effects of Wild Carrot Oil Extract

Abstract

Daucus carota L. ssp. carota (Apiacea) is used in traditional medicine in Lebanon and in different regions throughout the world. The present study investigates the in vitro anticancer activities of Daucus carota oil extract (DCOE) on four human cancer cell lines as well as its in vitro antioxidant activity. DCOE was extracted from the dried umbels with 50:50 acetone-methanol. The oil extract was analyzed by gas chromatography-mass spectrometry and screened for its antioxidant properties in vitro using 1,1-diphenyl-2-picryl hydrazyl free radical scavenging assay (DPPH), ferrous ion chelating assay (FIC) and the ferric reducing antioxidant power assay (FRAP). The anticancer activity of the oil extract against human colon (HT-29, Caco-2) and breast (MCF-7, MDA-MB-231) cancer cell lines was evaluated using the trypan blue exclusion method and the WST-1 cell proliferation assay. DCOE exhibited antioxidant activity in all assays used. The FRAP value was 164 ± 5.5 µmol FeSO4 /g, and the IC50 values for DPPH and FIC assays were 2.1 ± 0.03 mg/ml and 0.43 ± 0.02 mg/ml, respectively. Also, DCOE demonstrated a significant increase in cell death and decrease in cell proliferation. The effect of DCOE on the cell lines exhibited time and dose-dependent responses. The present study established that DCOE possesses both antioxidant and promising anticancer activities.

Home‐based preparation approaches altered the availability of health beneficial components from carrot and blueberry

“…Microwaving did not alter the DPPH • scavenging capacity of carrots. Previous studies reported 0.18 mg trolox equivalents per mL of carrot oil (Shebaby et al., 2013), which is about 0.025 μmol trolox equivalent per gram of carrot seeds with a 3.47% oil.…”

Nutritional and therapeutic importance of Daucus carota- A review

“…DCOE exhibited antioxidant activity in all assays. The FRAP value was 164 ± 5.5 µmol for FeSO 4 /g, and the IC 50 values for DPPH and FIC assays were 2.1 ± 0.03 mg/ml and 0.43 ± 0.02 mg/ml, respectively [109]. The antioxidant activity of the essential oils from the fruits of Daucus carota var.…”

“…Oil extract caused significant increase in cell death and decrease in cell proliferation. The effects of the oil on cell lines were time and dose-dependent [109].In cytotoxic investigation of the essential oils from the fruits of Daucus carota var. sativus (yellow carrot) and var.…”

Medicinal plants in the treatment ofHelicobacter pyloriinfections

“…Daucus carota L. (Apiacea) is traditionally used in different regions throughout the world for the treatment of gastric ulcer, diabetes, nephritic colic, cutaneous infections, and muscle pain (Jabrane et al, 2009;Shebaby et al, 2013). The in vitro anti-H. pylori properties of 60 different commercial essential oils were examined in a study.…”