The antioxidant activity of lemongrass leaves extract against fibroblasts oxidative stress

Abstract

Objective: The purpose of this research is to assess the antioxidant activity of lemongrass leaves extract in terms of lowering ROS generation and its effect on the viability and proliferation of fibroblasts under oxidative stress. Material and Methods: The antioxidant activity was measured using the DPPH method and the ROS assay was carried out by fluorescent H2DCFDA staining. Viability and proliferation assays were performed using the Cell Counting Kit-8 (CCK-8) and was read at 450 nm using microplate reader. The groups were divided into 8, namely fibroblasts without treatment (comparison group), fibroblast induced by H 2 O 2 (negative control), fibroblast with H 2 O 2 then treated with ascorbic acid (positive control), and fibroblast with H 2 O 2 then treated with lemongrass leaves extract at various concentrations (10, 20, 30, 40, and 50 ppm). Results: The results showed that the antioxidant activity of lemongrass leaves extract had an IC 50 value of 64.17 ppm. ROS production were reduced by the LgLE of all concentrations if compared with negative control (p=0.819). LgLE can maintained the fibroblast viability with 10 ppm of LgLE was the most optimum concentration (p<0.05). LgLE can induced the proliferation of fibroblast, with the most effective was at 24 h of observation (p<0.05). Conclusion: Lemongrass leaves extract has a strong antioxidant activity that can reduce oxidative stress and increase the viability and proliferation of fibroblasts with the optimum concentration is at 10 ppm.

Effects of Borassus flabellifer L. fruit seed coat on HSC-3 cell line

“…Cell viability was determined based on color changes of HSC-3 cell line treated with the CCK-8 reagent. Cell viability was determined using the following formula as followed : [13] % Viability of cells = absorbance of treated group absorbance of negative control…”

Chitosan Xylotrupes gideon encapsulated lemongrass leaf ethanol extract reduce H2O2‐induced oxidative stress in human dermal fibroblast

“…They were soaked in 70% ethanol at a 1:10 (w/v) ratio for 24 h at room temperature. The macerated mixture was filtered using a filter paper and evaporated with a rotary vacuum evaporator at a tempera ture of 40 °C at 100 rpm for 2 h (Fitria et al 2022).…”

“…Human dermal fibroblast (HDF) was obtained from the Biorepository of Stem Cell Research Center, Yarsi Univer sity, Indonesia (Fitria et al 2022). Fibroblast was planted in a cell culture dish and incubated at 37 °C with 5% CO 2 for 24 h. (Fitria et al 2022).…”

“…Previous studies show that Prunus avium L. extract encapsulation using nanochi tosan and gallic acid can decrease oxidative stress on en dothelium cells (Beconcini et al 2018) and 3T3 fibrob last cells (de Paiva et al 2021), respectively. Further more, the active compound encapsulation of lemongrass with chitosan polymer reduces ROS production of fibrob last by inducing hydrogen peroxide stressor (Fitria et al 2022). The observation with 2'7' dichlorodihydrofluo rescein diacetate (H2DCFDA) staining using a fluores cent microscope also shows the intensity of green fluores cent cells, indicating reduced ROS production (Andikop utri et al .…”