The antioxidant activity and free radical-scavenging capacity of dietary phenolic extracts from horse gram (Macrotyloma uniflorum (Lam.) Verdc.) seeds

Effects of processing conditions on the stability of polyphenolic contents and antioxidant capacity of Dolichos lablab L.

“…Hagerman et al (1998) reported that high molecular weight phenolics (tannins) have a greater ability to quench free radicals (ABTS ·+ ) and that effectiveness depends on the molecular weight, the number of aromatic rings and nature of hydroxyl groups substitution than the specific functional groups. Earlier, it was reported the ABTS ·+ radical cation scavenging activity in raw and dry heated samples of horse gram brown and black varieties (Siddhuraju and Manian 2007). On the other hand, the formation of tannin-protein complexes, both in insoluble and soluble complexes, as the result of conventional food/seed processing have also been shown to be potential free radical scavenger and radical sinks.…”

“…EC 50 means the effective concentration of sample that can decrease DPPH concentration by 50%. EC 50 was expressed as μg sample/ml been attributed to combined effects of Maillard reaction products (Siddhuraju and Manian 2007). The antioxidant properties of faba bean tannins indicated that the antioxidant activity was accounted for by the direct interaction of tannin with hydroxyl radical rather than to a metal chelating activity (Carbonaro et al 1996).…”

Antidiabetic potential of α-amylase inhibitor from the seeds ofMacrotyloma uniflorumin streptozotocin-nicotinamide-induced diabetic mice

“…(Kawsar et al, 2009;Siddhuraju & Manian, 2007). One of the major problems associated with diabetes is postprandial hyperglycemia.…”

A comparative study on in vitro antioxidant activity of the legumesAcacia auriculiformisandAcacia ferrugineawith a conventional legumeCajanus cajanEstudio comparativo de la actividad antioxidante in vitro de las legumbres AcaciaauriculiformisyAcacia ferrugineacon la legumbre convencionalCajanus cajan

“…ABTS radical cation (ABTS .þ ) was produced by reacting ABTS stock solution with 2.45 mM potassium persulfate (final concentration) and allowing the mixture to stand in the dark at room temperature for 12-16 h before use. Prior to assay, the solution was diluted in ethanol (about 1:89 v/v) and equilibrated to 30 8C to give an absorbance at 734 nm of 0.700 + 0.02 in a 1 cm cuvette (Siddhuraju & Manian, 2007). The concentration of sample extract that produced between 20%-80% inhibition of the blank absorbance was determined and adapted.…”