The antigens contributing to the serological cross-reactions of Proteus antisera with Klebsiella representatives

Palusiak, Agata

doi:10.1016/j.molimm.2014.11.016

Cited by 6 publications

(14 citation statements)

References 20 publications

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“…LPSs from dry masses of planktonic and biofilm-forming bacteria were extracted using the phenol–water method by Westphal and Jann [ 24 ], modified as described by Palusiak [ 25 ]. They were subsequently used for silver staining, stimulation of THP-1 cells, and as antigens in serological assays, as well as for the chemical analysis of O-polysaccharides, core regions, and lipids A.…”

Section: Methodsmentioning

confidence: 99%

Changes in the lipopolysaccharide of Proteus mirabilis 9B-m (O11a) clinical strain in response to planktonic or biofilm type of growth

Zabłotni

Matusiak

Arbatsky

et al. 2018

Med Microbiol Immunol

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The impact of planktonic and biofilm lifestyles of the clinical isolate Proteus mirabilis 9B-m on its lipopolysaccharide (O-polysaccharide, core region, and lipid A) was evaluated. Proteus mirabilis bacteria are able to form biofilm and lipopolysaccharide is one of the factors involved in the biofilm formation. Lipopolysaccharide was isolated from planktonic and biofilm cells of the investigated strain and analyzed by SDS–PAGE with silver staining, Western blotting and ELISA, as well as NMR and matrix-assisted laser desorption ionization time-of-flight mass spectrometry techniques. Chemical and NMR spectroscopic analyses revealed that the structure of the O-polysaccharide of P. mirabilis 9B-m strain did not depend on the form of cell growth, but the full-length chains of the O-antigen were reduced when bacteria grew in biofilm. The study also revealed structural modifications of the core region in the lipopolysaccharide of biofilm-associated cells—peaks assigned to compounds absent in cells from the planktonic culture and not previously detected in any of the known Proteus core oligosaccharides. No differences in the lipid A structure were observed. In summary, our study demonstrated for the first time that changes in the lifestyle of P. mirabilis bacteria leads to the modifications of their important virulence factor—lipopolysaccharide.

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Section: Methodsmentioning

confidence: 99%

Changes in the lipopolysaccharide of Proteus mirabilis 9B-m (O11a) clinical strain in response to planktonic or biofilm type of growth

Zabłotni

Matusiak

Arbatsky

et al. 2018

Med Microbiol Immunol

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“…LPSs (P. penneri 2 (O66), 22 (O63), and 60 (O70) and P. vulgaris CCUG 4677 (O42)) came from the collection of the Laboratory of General Microbiology where they are stored at −20°C. LPSs were extracted from dry biomasses of K. pneumoniae 0.08, K. oxytoca 0.023, 0.030, and 0.042 strains in 45% phenol for 5 min at 65°C using the phenol-water method, developed by Westphal and Jann (1965) with modifications described by Palusiak (2015). The water phase containing LPS with long OPS chains was collected after centrifugation (4,845×g) and the extraction was repeated.…”

Section: Bacterial Antigens and Sera Obtainingmentioning

confidence: 99%

“…In the previous studies, the reactions of nine Proteus antisera and the cells of selected Klebsiella spp. clinical isolates were described (Palusiak, 2015). Most of the Western blotting reactions concerned protein antigens.…”

Section: Introductionmentioning

confidence: 99%

The Contribution of Polysaccharide Antigens From Clinical Proteus spp. and Klebsiella spp. Isolates to the Serological Cross-Reactions

Palusiak

2021

Front. Cell. Infect. Microbiol.

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Klebsiella spp. and Proteus spp. cause hospital-acquired urinary tract infections (UTIs), which are often related to the use of catheters. To create a vaccine preventing UTI, immunogenic bacterial antigens with common epitopes are still being looked for. In this work, the role of polysaccharide antigens of four Klebsiella spp. and eight Proteus spp. strains in serological cross-reactions with specific antisera was examined. Enzyme-linked immunosorbent assay (ELISA), Western blotting, and silver staining by Tsai method were performed. The Klebsiella and Proteus spp. LPSs and cells were used as antigens. Polyclonal rabbit sera specific to Klebsiella oxytoca 0.023 and 0.062 strains and four Klebsiella spp. LPSs were obtained. The ELISA and Western blotting results showed the strongest cross-reactions occurring between lipopolysaccharides (LPSs) from four Klebsiella strains and P. vulgaris O42 antiserum. The silver-staining procedure revealed the patterns typical of both slow- and fast-migrating mass species of the Klebsiella LPSs. The Klebsiella spp. antigens also cross-reacted with four P. penneri antisera, and most of the reactions were observed as low-migrating patterns. From two K. oxytoca antisera obtained in this work, only one, the K. oxytoca 0.062 antiserum, cross-reacted with satisfactory strength with P. penneri LPSs (19, 22, and 60). Obtaining cross-reactions between the antigens of Klebsiella strains and Proteus antisera and in the opposite systems is important for proving the immunogenic role of polysaccharide antigens in triggering the immunological response.

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“…2a,b). By the degradation of proteins, we could remove possible non-speci c cross reactions caused by protein antigens rather than by O antigens [26]. Indeed, the initially observed in ELISA reactions of several biomasses not fully adsorbing the respective antisera, could have been caused by protein antigens, as they were not con rmed in Western blots.…”

Section: Serological Featuresmentioning

confidence: 99%

The Prevailing O Serogroups Among the Serologically Differentiated Clinical Proteus Spp. Strains in Poland

Drzewiecka

Palusiak

Siwińska

et al. 2021

Preprint

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In the years 2006-2011, 617 Proteus spp. strains isolated mostly from urine and wounds or other clinical sources from infected individuals were collected in Łódź, Poland. P. mirabilis species was dominating (86.9%), followed by P. genomospecies, P. vulgaris , and P. penneri . Ninety four per cent strains were recognized as S (smooth) forms. P. mirabilis exhibited the most intensive swarming growth. Serological studies (involving ELISA – enzyme-linked immunosorbent assay and Western blotting using native and adsorbed rabbit antisera) enabled classification of 80% S isolates into respective Proteus O serogroups among the 83 ones, described so far. The remaining strains seemed to be serologically unique. Detailed serological analysis confirmed that the recently described in Poland O78 serogroup is the most widespread. Also, 14 other serogroups have been found to predominate, being represented by ten or more strains. No unique structural feature of the prevalent O serotypes has been indicated. The observed big serological variety may suggest a low rate of Proteus spp. strains’ transmission between patients or rapid changes in their lipopolysaccharides. However, the prevalence of some O serogroups indicates that particular serotypes may be in some ways beneficial to the strains producing these kinds of O antigen.

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The antigens contributing to the serological cross-reactions of Proteus antisera with Klebsiella representatives

Cited by 6 publications

References 20 publications

Changes in the lipopolysaccharide of Proteus mirabilis 9B-m (O11a) clinical strain in response to planktonic or biofilm type of growth

Changes in the lipopolysaccharide of Proteus mirabilis 9B-m (O11a) clinical strain in response to planktonic or biofilm type of growth

The Contribution of Polysaccharide Antigens From Clinical Proteus spp. and Klebsiella spp. Isolates to the Serological Cross-Reactions

The Prevailing O Serogroups Among the Serologically Differentiated Clinical Proteus Spp. Strains in Poland

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