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“…Since LDH C4 is a relatively abundant testicular enzyme, LDH C4 mRNA represents as much as 0.18% of total functional mRNA in mouse pachytene spermatocytes (11). Because LDH C4 is also present on the surface of mature spermatozoa, it has been used extensively in immunocontraceptive efforts (12). Although the protein sequence of LDH C4 from rodents has been known for some time (13), only recently has a cDNA probe for human LDH C4 been isolated (14).…”
Section: Meiotic Dna Probesmentioning
“…However, an anti-somatic tubulin serum did react with the 58-kilo- (1:50) a DT (Connaught Laboratories) was activated with 6-maleimidocaproic acyl N-hydroxy succinimide (9). A solid-matrix radioimmunoassay was used to test samples for antibodies to the peptide (17 (Fig. 4A).…”
mentioning