The antibiotics supplemented bone cement improved the masquelet's induced membrane in a rat femur critical size defect model

Koluman, Alican; Ziroğlu, Nezih; Kaleci, Belisa; Tanrıverdi, Bülent; Tanrıverdi, Gamze; Kural, Alev; Bilgili, Mustafa Gökhan

doi:10.1016/j.injury.2022.10.027

Cited by 2 publications

(9 citation statements)

References 45 publications

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“…The mean score on the ARRIVE Essential 10 checklist was 7.03 ± 0.97 (minimum–maximum 5–8) and the mean total score was 14.6 ± 1.8 (minimum–maximum 11–18) (Supplementary Table 1). The frequently missing items of the Essential Set were the description of the randomization method that was done partially or totally in 13 (65%) studies ( 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 ) and the blinding assessment of the study’s endpoints that were only done in one (5%) study ( 12 ). Finally, the a priori calculation of the number of animals needed for statistical comparison was only done in 5 (25%) studies ( 10 , 12 , 17 , 20 , 21 ).…”

Section: Resultsmentioning

confidence: 99%

“…The most frequent strain was the outbred Sprague-Dawley rat (16/20) ( 8 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 19 , 21 , 22 , 23 , 24 , 25 , 26 ), followed by Wistar (3/20) ( 9 , 20 , 27 ) and one study used an inbred 344 Fischer strain ( 18 ). Only 2/20 studies used female rats (Wistar) ( 9 , 27 ).…”

Section: Resultsmentioning

confidence: 99%

“…The mean length of the femoral defect was 7 ± 2.2 mm (minimum–maximum 4–10). Stabilization of femoral defect was principally achieved with one plate and 4 screws (13/20) ( 9 , 10 , 12 , 13 , 14 , 17 , 18 , 19 , 20 , 22 , 23 , 24 , 27 ) or 6 screws (1/20) ( 26 ), followed by external fixation (4/20) ( 11 , 15 , 21 , 25 ) and intramedullary nailing (2/20) ( 8 , 16 ). Two studies used an additional cerclage wiring ( 9 , 26 ).…”

Section: Resultsmentioning

confidence: 99%

“…The main strategy consisted of an optimization of the conventional two-stage IMT (13/20) ( 8 , 10 , 11 , 14 , 15 , 17 , 18 , 19 , 20 , 21 , 24 , 25 , 27 ). A part of them (4/13) loaded the PMMA spacer with bioactive molecules such as antibiotics and growth factors ( 10 , 18 , 20 , 25 ). One study administered an osteoinductive drug orally ( 14 ).…”

Section: Resultsmentioning

confidence: 99%

“…Four studies functionalized the PMMA to improve the IM properties. At 4 weeks, PMMA + gentamicin improved vascularization or TGFB1 expression vs PMMA + gentamicin and clindamycin or vs a high viscosity PMMA ( 10 , 20 ). Oral administration of total flavonoid of rhizoma drynariae also showed to improve growth factors expressions of the IM ( 14 ).…”

Section: Outcomes Of the Induced Membrane Technique Optimization Stra...mentioning

confidence: 95%

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Systematic literature review of in vivo rat femoral defect models using biomaterials to improve the induced membrane technique: a comprehensive analysis

Saab,

Zobrist,

Blanchemain

et al. 2024

EFORT Open Reviews

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Purpose The aim of this study was to conduct a systematic literature review analyzing the results of in vivo rat femoral defect models using biomaterials for improving the induced membrane technique (IMT). Methods Following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines, the PubMed, Embase, and Web of Science databases were searched. Inclusion criteria were studies reporting results of the IMT in in vivo rat femoral critical-sized defect models using a biomaterial possibly combined with molecules. Methodologic quality was assessed with the Animal Research: Reporting In Vivo Experiments guidelines. Results Twenty studies met the inclusion criteria. Femoral stabilization with plate and screws was the most frequent. Histologic, biomechanical, and/or radiologic analyses were performed. In two-stage strategies, the PMMA spacer could be associated with bioactive molecules to enhance IM growth factor expression and improve bone formation. Modulating the roughness of spacers could increase IM thickness and accelerate its formation. In one-stage strategies, human tissue-derived membranes combined with bone grafting achieved bone formation comparable to a standard IMT. All calcium phosphate grafts seemed to require a functionalization with growth factors or bone marrow mononuclear cells to improve outcomes compared with non-functionalized grafts. Conclusion This systematic review described the main parameters of the in vivo rat femoral defect models using biomaterials to improve the induced membrane technique. Although the studies included had several methodological limitations that may limit the scope of these conclusions, one- and two-stage strategies reported promising results with biomaterials to improve the IMT.

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