The anti-tumor activities of cerebrosides derived from sea cucumber &lt;i&gt;Acaudina molpadioides&lt;/i&gt; and starfish &lt;i&gt;Asterias amurensis in vitro&lt;/i&gt; and &lt;i&gt;in vivo&lt;/i&gt;

Du, Lei; Li, Zhaojie; Xu, Jie; Wang, Jingfeng; Xue, Yong; Xue, Changhu; Takahashi, Kyoko; Wang, Yuming

doi:10.5650/jos.61.321

Cited by 34 publications

(24 citation statements)

References 41 publications

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“…After cDNA synthesis, quantitative RT-PCR was performed in the Bio-Rad iCycler iQ5 system as described previously 24 . The gene expression was determined by relative quantification using the standard curve method.…”

Section: Rna Extraction and Quantitative Real Time Pcrmentioning

confidence: 99%

Comparative Study of EPA-enriched Phosphatidylcholine and EPA-enriched Phosphatidylserine on Lipid Metabolism in Mice

et al. 2016

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Section: Rna Extraction and Quantitative Real Time Pcrmentioning

confidence: 99%

Comparative Study of EPA-enriched Phosphatidylcholine and EPA-enriched Phosphatidylserine on Lipid Metabolism in Mice

et al. 2016

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“…Recent reports have shown that sea cucumber Stichopus variegates sphingoid bases e.g. d17:1, 4, 8-sphingadienine d18:2 , d18:3 and d19:3 have strong cytotoxicity against some colon cancer cell lines via induction of apoptosis 39 , and the cerebrosides derived from the sea cucumber Acaudina molpadioides and the starfish Asterias amurensis had anti-tumor activity in vitro and in vivo, the different effect might depend on their different structure 21 . The soya-cerebroside exhibited a Ca 2 -binding activity in a glass-cell apparatus W-08 and a Ca 2 -permeation ability across the human erythrocyte membrane, induced nerve damage, and the main long-chain base was C18-4, 8-diunsaturated 40 .…”

Section: Disscusionmentioning

confidence: 99%

“…Several studies have shown that the global shutdown of mitochondrial function under conditions of oxidative stress could contribute to apoptosis 33 . Bcl-2 family genes regulate apoptosis mainly via the mitochondrial pathway 21 . Bcl-2 is the first mammalian regulator gene that was identified to have anti-apoptotic potential in a variety of cell systems, intracellular Bcl-2 expression increases can block cell apoptosis related with ROS 34 .…”

Section: Disscusionmentioning

confidence: 99%

“…1 μg of total RNA from each sample was converted to first strand cDNA using MMLV reverse transcriptase and random primers. Quantitative real-time PCR assay was carried out as described previously 21 . The prime sequences are listed in Table 1.…”

Section: Rna Preparation and Quantitative Real-time Pcrmentioning

confidence: 99%

“…The glycosphingolipids of marine invertebrates, including sea cucumber and starfish, have unique sphingoid bases with a conjugated diene 19 . Recent research have found that sea cucumber cerebrosides SCC can attenuate the rat fatty liver induced by orotic acid 20 , and starfish cerebrosides SFC has significant antitumor effects in vitro and vivo 21 .…”

Section: Introductionmentioning

confidence: 99%

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The Protective Effects of Cerebrosides from Sea Cucumber and Starfish on the Oxidative Damage in PC12 Cells

Xue

Tang

et al. 2013

J. Oleo Sci.

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Reversed‐Phase Liquid Chromatography–Quadrupole‐Time‐of‐Flight Mass Spectrometry for High‐Throughput Molecular Profiling of Sea Cucumber Cerebrosides

Jia

Cong

Zhang

et al. 2015

Lipids

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Usually, the chemical structures of cerebrosides in sea creatures are more complicated than those from terrestrial plants and animals. Very little is known about the method for high-throughput molecular profiling of cerebrosides in sea cucumbers. In this study, cerebrosides from four species of edible sea cucumbers, specifically, Apostichopus japonicas, Thelenota ananas, Acaudina molpadioides and Bohadschia marmorata, were rapidly identified using reversed-phase liquid chromatography-quadrupole-time-of-flight mass spectrometry (RPLC-QToF-MS). [M + H](+) in positive electrospray ionization (ESI) mode were used to obtain the product ion spectra. The cerebroside molecules were selected according to the neutral loss fragments of 180 Da and then identified according to pairs of specific products of long-chain bases (LCB) and their precursor ions. A typical predominant LCB was 2-amino-1,3-dihydroxy-4-heptadecene (d17:1), which was acylated to form saturated and monounsaturated non-hydroxy and monohydroxy fatty acids with 17-25 carbon atoms. Simultaneously, the occurrence of 2-hydroxy-tricosenoic acid (C23:1h) was characteristic of sea cucumber cerebrosides, whereas this molecule was rarely discovered in plants, mammals, or fungi. The profiles of LCB and fatty acids (FA) distribution might be related to the genera of sea cucumber. These data will be useful for identification of cerebrosides using RPLC-QToF-MS.

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The anti-tumor activities of cerebrosides derived from sea cucumber <i>Acaudina molpadioides</i> and starfish <i>Asterias amurensis in vitro</i> and <i>in vivo</i>

Cited by 34 publications

References 41 publications

Comparative Study of EPA-enriched Phosphatidylcholine and EPA-enriched Phosphatidylserine on Lipid Metabolism in Mice

Comparative Study of EPA-enriched Phosphatidylcholine and EPA-enriched Phosphatidylserine on Lipid Metabolism in Mice

The Protective Effects of Cerebrosides from Sea Cucumber and Starfish on the Oxidative Damage in PC12 Cells

Reversed‐Phase Liquid Chromatography–Quadrupole‐Time‐of‐Flight Mass Spectrometry for High‐Throughput Molecular Profiling of Sea Cucumber Cerebrosides

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