IntroductionThe genus Cyprinion (type species: Cyprinion macrostomus Heckel, 1843) is a western Asian genus of minnow, distributed from western Syria and the southern Arabian Peninsula to the western tributaries of the Indus River in Punjab (Pakistan). Including the 3 species of Cyprinion inhabiting the Tigris-Euphrates Basin, 5 species have been observed in Iran (Coad, 1995), but their taxonomic positions have not been fully resolved yet. However, distributions and morphological characteristics of C. macrostomus and C. kais have been investigated in Turkey by Ünlü (1999). Furthermore, karyotypes of 3 species, C. tenuiradius, C. macrostomus, and C. kais, have been studied (Esmaeili and Piravar, 2006; Nasri et al., unpublished data). The karyological analysis of C. macrostomus has been examined as well (Gaffaroğlu and Yüksel, 2004). Daştan et al. (2012) reported the genetic diversity of C. macrostomus. The revision of C. macrostomus and C. kais was studied by Banarescu and Straschil (1995). The length-weight and length-length relationships of C. macrostomus have been determined (Sedaghat and Hoseini, 2012;Bibak et al., 2013). There has been no study on the reproduction characteristics of C. macrostomus or the growth and reproduction characteristics of C. kais in the literature. Failure to study various aspects of these species in order to take necessary conservation measures may lead to depletion of its populations in the near future. C. macrostomus is an edible and valuable species for sport fishing (Abdoli, 2000) and is also used as an aquarium fish. It is called the "doctor fish", because it plays a therapeutic role in medical treatment (Ündar et al., 1990), and it is also known as "stone fish" due to its feeding activities. Cyprinion kais, an endemic freshwater fish in the Tigris-Euphrates Basin, is distributed in inland waters of Iran,