The Allium cepa chromosome aberration test reliably measures genotoxicity of soils of inhabited areas in the Ukraine contaminated by the Chernobyl accident

Kovalchuk, Olga; Kovalchuk, Igor; Arkhipov, Andrey; Telyuk, P.; Höhn, Barbara; Kovalchuk, L. Ye.

doi:10.1016/s1383-5718(98)00053-9

Cited by 101 publications

(63 citation statements)

References 21 publications

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“…Darlington and Mc-Leish (1951) proposed that stickiness might be due to degradation or depolymerization of chromosomal DNA. Besides, many researchers reported that its occurrences during the study could be caused of subchromatid linkage between chromosomes (Chauhan et al 1986;Ajay and Sarbhoy 1988;Kovalchuk et al 1998;Liman et al 2010). The bridges involving one or more chromosomes were the most important and frequent type in addition to sticky chromosomes.…”

Section: Discussionmentioning

confidence: 94%

Potential cytotoxic effect of Anilofos by using Allium cepa assay

et al. 2014

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Cytogenetic effects of Anilofos which was widely used in agriculture, was evaluated in Allium cepa root meristematic cells. In the Allium root growth inhibition test EC 50 value was determined 50 ppm and 1/29 EC 50 (25 ppm), EC 50 (50 ppm) and 2 9 EC 50 (100 ppm) concentrations of Anilofos were applied to onion roots. A negative and positive control were used in the experiment in parallel. According to results mitotic index decreased with increasing the Anilofos concentrations in all application groups and each exposure time, while disturbed anaphase-telophase, choromosome laggard(s), stickiness and anaphase bridge(s) were observed. In anaphase-telophase cells, c-metaphase, disturbed nucleus and binuclear cells were observed in other anomalies. The results were also analyzed statistically by using Dunnett t test (2-tailed) and all concentrations of Anilofos were found significant.

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Section: Discussionmentioning

confidence: 94%

Potential cytotoxic effect of Anilofos by using Allium cepa assay

et al. 2014

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“…Chromosome aberration test concerns the discovery of the mechanisms of action of a particular agent, since the division process is well known. Kovalchuk et al (1998) state that the chromosome aberrations assay with A. cepa, can be used as a tool for quantifying and monitoring genetic alterations in soils radioactively contaminated. Moreover, the chromosome aberration assay in onion was the first of nine plant systems accepted in the Genotoxic Program of the Environmental Protection Agency (USEPA) and is widely used for monitoring residual water.…”

Section: Genotoxicity Biomarkersmentioning

confidence: 99%

Bioindicators and Biomarkers in the Assessment of Soil Toxicity

Fontanetti¹,

Nogarol²,

Souza³

et al. 2011

Soil Contamination

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“…Analysis of the chromosomes showed that 7.5 g/L of Lg-ext concentration except in 12 and 24 h and 15 g/L of Lg-ext concentration in 12 and 96 h induced chromosomal aberrations but other Lg-ext concentrations decreased chromosomal aberrations. No aberration was recorded in the chromosome of A. cepa exposed to the 30 g/L of Lg-ext concentration in 72 and 96 h. Stickiness (especially at 7.5 g/L in 48 h) indicates highly irreversible type toxic effect of Lg-ext, and its occurrences during the study could be because of subchromatid linkage between chromosomes (Mc-Gill et al, 1974;Chauhan et al, 1986;Kovalchuk et al, 1998;Ajay & Sarbhoy, 1988). Anaphase bridges could happen during the translocation of the unequal chromatid exchange or due to dicentric chromosome presence or due to the breakage and fusion of chromosomes and chromatids.…”

Section: Resultsmentioning

confidence: 84%

Evaluation of genotoxic and mutagenic effects of aqueous extract from aerial parts of Linaria genistifolia subsp. genistifolia

Liman¹,

Gökçe²,

Akyıl³

et al. 2012

Rev. bras. farmacogn.

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Genotoxic and mutagenic effects of aqueous extract from aerial parts Linaria genistifolia (L.) Mill. subsp. genistifolia, Plantaginaceae (Lg-ext) were investigated by using both Allium cepa root meristematic cells and bacterial reverse mutation assay in Salmonella typhimurium TA98 and TA100 with or without metabolic activation system (S9), respectively. In Allium root growth inhibition test, EC50 value was determined approximately 15 g/L and 0.5xEC50, EC50 and 2xEC50 concentrations of Lg-ext were introduced to onion tuber roots and distilled water and methyl methane sulfonate (MMS, 10 ppm) used as a negative and positive control, respectively. The characteristic effect caused by tested preparations was an increase of mitotic index (MI) in 7.5 g/L and 15 g/L (except 24 and 96 h) and simultaneous decrease of MI in 30 g/L and in MMS. While stickiness, bridges, chromosome laggards and disturbed anaphase-telophase were observed in anaphase-telophase cells, c-metaphase, pro-metaphase, polyploidy and binuclear cells were observed in other cells. Lg-ext was not found to be mutagenic on S. typhimurium TA 98 and TA100 with or without S9. The results were also analyzed statistically by using SPSS for Windows, and Duncan's multiple range tests were performed respectively. These results indicate that Lg-ext exhibits genotoxic activity in A. cepa root meristematic cells but not mutagenic activity in Ames test system.

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The Allium cepa chromosome aberration test reliably measures genotoxicity of soils of inhabited areas in the Ukraine contaminated by the Chernobyl accident

Cited by 101 publications

References 21 publications

Potential cytotoxic effect of Anilofos by using Allium cepa assay

Potential cytotoxic effect of Anilofos by using Allium cepa assay

Bioindicators and Biomarkers in the Assessment of Soil Toxicity

Evaluation of genotoxic and mutagenic effects of aqueous extract from aerial parts of Linaria genistifolia subsp. genistifolia

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