The Alfalfa (<i>Medicago sativa</i>) <i>TDY1</i> Gene Encodes a Mitogen-Activated Protein Kinase Homolog

Schoenbeck, Mark A.; Samac, Deborah A.; Fedorova, Maria; Gregerson, Robert G.; Gantt, J. Stephen; Vance, Carroll P.

doi:10.1094/mpmi.1999.12.10.882

Cited by 40 publications

(26 citation statements)

References 58 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Induction of MAP kinases by fungal elicitors has been shown (Ligterink et al, 1997;Lebrun-Garcia et al, 1998) and transcripts of the MAP kinase gene Tdy1 was predominantly detected in the parenchymatic and meristematic regions of the nodule (Schoenbeck et al, 1999). The cDNA fragment identified here shows 100% identity on the nucleic acid level to a MAP kinase gene which can be detected in all tissues of P. sativum (Stafstrom et al, 1993) and which is involved in cell cycle regulation in Medicago sativa (Jonak et al, 1993).…”

Section: Ephmentioning

confidence: 65%

Identification of mycorrhiza-regulated genes with arbuscule development-related expression profile

et al. 2004

View full text Add to dashboard Cite

Suppressive subtractive hybridisation was applied to the analysis of late stage arbuscular mycorrhizal development in pea. 96 cDNA clones were amplified and 81, which carried fragments more than 200 nt in size, were sequence analysed. Among 67 unique fragments, 10 showed no homology and 10 were similar to sequences with unknown function. RNA accumulation of the corresponding 67 genes was analysed by hybridisation of macro-arrays. The cDNAs used as probes were derived from roots of wild type and late mutant pea genotypes, inoculated or not with the AM fungus Glomus mosseae. After calibration, a more than 2.5-fold mycorrhiza-induced RNA accumulation was detected in two independent experiments in the wild type for 25 genes, 22 of which seemed to be induced specifically during late stage AM development. Differential expression for 7 genes was confirmed by RT-PCR using RNA from mycorrhiza and from controls of a different pea cultivar. In order to confirm arbuscule-related expression, the Medicago truncatula EST data base was screened for homologous sequences with putative mycorrhiza-related expression and among a number of sequences with significant similarities, a family of trypsin inhibitor genes could be identified. Mycorrhiza-induced RNA accumulation was verified for five members by real-time PCR and arbuscule-related activation of the promoter could be shown in transgenic roots for one of the genes, MtTi 1.

show abstract

Section: Ephmentioning

confidence: 65%

Identification of mycorrhiza-regulated genes with arbuscule development-related expression profile

et al. 2004

View full text Add to dashboard Cite

show abstract

“…27,28 One another study showed that a gene called TDY1, believed to encode a MAPK homolog, was involved in root nodulation and root-tip development in Medicago. 29 One of the well-studied MAPKs in legumes is MAPK4 ortholog in soybean: this gene is involved in negative regulation of defense mechanism while in positive regulation of growth and development. 30 MAPKs in legumes are known to involve in various signaling responses that involve root organogenesis-for example, in Lupinus albus, activation of MAPKs such as SIMK and SAMK are required for infection of Bradyrhizobia sp.…”

Section: Introductionmentioning

confidence: 99%

Evolutionary history of mitogen-activated protein kinase (MAPK) genes inLotus,Medicago, andPhaseolus

Neupane

Nepal

Benson

et al. 2013

Plant Signaling & Behavior

View full text Add to dashboard Cite

“…To date, no mutant phenotypes have been ascribed to any MAP kinase gene of Arabidopsis or of any other plant. However, several plant MAP kinases have been identified that are activated by pathogen infection and by related signal molecules (35)(36)(37)(38)(39)(40)(41). For example, the tobacco MAP kinase SIPK is activated by tobacco mosaic virus infection, SA, by several pathogen-derived elicitors and by wounding (42)(43)(44).…”

Section: Identification Of Edr1 Orthologs In Crop Speciesmentioning

confidence: 99%

From the Cover: Negative regulation of defense responses in plants by a conserved MAPKK kinase

Frye

Tang²,

Innes³

2000

Proceedings of the National Academy of Sciences

216

View full text Add to dashboard Cite

The enhanced disease resistance 1 (edr1) mutation of Arabidopsis confers resistance to powdery mildew disease caused by the fungus Erysiphe cichoracearum. Resistance mediated by the edr1 mutation is correlated with induction of several defense responses, including host cell death. Double mutant analysis revealed that all edr1-associated phenotypes are suppressed by mutations that block salicylic acid (SA) perception (nim1) or reduce SA production (pad4 and eds1). The NahG transgene, which lowers endogenous SA levels, also suppressed edr1. In contrast, the ein2 mutation did not suppress edr1-mediated resistance and associated phenotypes, indicating that ethylene and jasmonic acid-induced responses are not required for edr1 resistance. The EDR1 gene was isolated by positional cloning and was found to encode a putative MAP kinase kinase kinase similar to CTR1, a negative regulator of ethylene responses in Arabidopsis. Taken together, these data suggest that EDR1 functions at the top of a MAP kinase cascade that negatively regulates SA-inducible defense responses. Putative orthologs of EDR1 are present in monocots such as rice and barley, indicating that EDR1 may regulate defense responses in a wide range of crop species. P lants defend themselves against infectious diseases by using both preformed and induced defenses. The latter comprise a complex suite of physiological changes, including a form of programmed cell death called the hypersensitive resistance response (HR) (1). In an effort to identify plant genes that regulate defense responses, we screened for Arabidopsis mutants that displayed enhanced resistance to normally virulent pathogens. The edr1 mutant was identified in a screen for mutants that had become resistant to the bacterium Pseudomonas syringae and was subsequently shown to be resistant to Erysiphe cichoracearum (powdery mildew) (2). Significantly, edr1 mutant plants do not display constitutive expression of the defense gene PR-1, indicating that resistance is not caused by constitutive activation of systemic acquired resistance-associated defenses (3).Although known defense responses are not constitutively expressed in edr1 plants, several defense responses are induced by E. cichoracearum more rapidly in edr1 plants than in wild-type Arabidopsis variety Col-0 (2). These include deposition of autofluorescent compounds and callose [a ␤-(133) glucan] in mesophyll cell walls, accumulation of PR-1 mRNA, and mesophyll cell death. In wild-type Col-0 plants infected with E. cichoracearum, these defenses are induced more slowly, and very little cell death is observed (2, 4). Because the edr1 mutation is recessive, the EDR1 gene appears to function as a negative regulator. Because these defenses are not expressed in edr1 plants in the absence of pathogens, however, there must be pathogen-associated signals required to induce these defenses. In the absence of EDR1 function, even presumably weak signals from virulent Erysiphe strains are sufficient to induce strong responses.Because the edr1 mutant appears p...

show abstract

The Alfalfa (Medicago sativa) TDY1 Gene Encodes a Mitogen-Activated Protein Kinase Homolog

Cited by 40 publications

References 58 publications

Identification of mycorrhiza-regulated genes with arbuscule development-related expression profile

Identification of mycorrhiza-regulated genes with arbuscule development-related expression profile

Evolutionary history of mitogen-activated protein kinase (MAPK) genes inLotus,Medicago, andPhaseolus

From the Cover: Negative regulation of defense responses in plants by a conserved MAPKK kinase

Contact Info

Product

Resources

About