The Airway Microbiota in Cystic Fibrosis: A Complex Fungal and Bacterial Community—Implications for Therapeutic Management

Delhaès, Laurence; Monchy, Sébastien; Fréalle, Émilie; Hubans, Christine; Salleron, Julia; Leroy, Sylvie; Prévotat, A.; Wallet, F.; Wallaert, B.; Dei‐Cas, Eduardo; Sime-Ngando, Télesphore; Chabé, Magali; Viscogliosi, Éric

doi:10.1371/journal.pone.0036313

Cited by 320 publications

(344 citation statements)

References 103 publications

Supporting

Mentioning

302

Contrasting

Unclassified

Order By: Relevance

“…Quelques études se sont intéressées au mycobiote digestif ou cutané [1,2], très peu ont abordé le mycobiote pulmonaire [3][4][5][6][7]. Pourtant, plusieurs éléments démontrent l'importance de la flore fongique dans les pathologies respiratoires chroniques telles que la broncho-pneumopathie chronique obstructive (BPCO), l'asthme ou la mucoviscidose : (1) les spores (ou conidies) de champignons sont de petite taille (2 à 10 μm), très facilement inhalées.…”

Section: (➜)unclassified

“…À ce jour, quelques études ont analysé et identifié le mycobiote pulmonaire du sujet sain et/ou les profils de mycobiote modifié (ou dysbiose) caractérisant un contexte pathologique spécifique (asthme, mucoviscidose et transplantation pulmonaire). En utilisant les approches de séquençage à haut débit, toutes ces études ont mis en évidence une flore fongique beaucoup plus riche que celle identifiée par méthode conventionnelle (culture et/ou PCR ciblant un pathogène) [3][4][5][6][7]. En effet, ces techniques offrent la possibilité théorique d'identifier toutes les espèces fongiques pré-sentes (y compris celles réfractaires à la culture comme Pneumocystis jirovecii) et de générer des données détaillées, précises et sans a priori sur cette communauté microbienne [3][4][5][6][7].…”

Section: (➜)unclassified

“…En utilisant les approches de séquençage à haut débit, toutes ces études ont mis en évidence une flore fongique beaucoup plus riche que celle identifiée par méthode conventionnelle (culture et/ou PCR ciblant un pathogène) [3][4][5][6][7]. En effet, ces techniques offrent la possibilité théorique d'identifier toutes les espèces fongiques pré-sentes (y compris celles réfractaires à la culture comme Pneumocystis jirovecii) et de générer des données détaillées, précises et sans a priori sur cette communauté microbienne [3][4][5][6][7]. Chez les sujets sains [1,2,4,6], le mycobiote pulmonaire comporte de nombreux champignons issus de l'environnement dont des Aspergillus, des Cladosporium, des Penicillium et beaucoup de levures (Figure 1 Mieux connaître la communauté fongique pulmonaire et son écologie, la comparer avec celle de la communauté bactérienne, permettra de mieux analyser les synergies potentielles entre microorganismes.…”

Section: (➜)unclassified

“…Les caractéristiques écologiques de ce mycobiote (diversité et richesse des espèces fongiques) semblent corrélées à l'évolution clinique des patients : un statut clinique peu favorable est en effet associé à une moins grande diversité et richesse fongique [4,5]. De façon surprenante, les espèces du genre Malassezia ont été isolées en grande quantité chez ces patients [5,7].…”

Section: Perspectives : Vers Une Intégration Du Mycobiome Pulmonaireunclassified

“…De façon surprenante, les espèces du genre Malassezia ont été isolées en grande quantité chez ces patients [5,7]. L'espèce Malassezia pachydermatis, responsable de troubles allergiques, a notamment été isolée chez les patients asthmatiques au cours d'une étude castémoins [6] (Figure 1).…”

Section: Perspectives : Vers Une Intégration Du Mycobiome Pulmonaireunclassified

See 4 more Smart Citations

Un nouveau concept

Nguyen

Delhaès

2015

Med Sci (Paris)

Self Cite

View full text Add to dashboard Cite

Section: (➜)unclassified

Section: Perspectives : Vers Une Intégration Du Mycobiome Pulmonaireunclassified

See 3 more Smart Citations

Un nouveau concept

Nguyen

Delhaès

2015

Med Sci (Paris)

Self Cite

View full text Add to dashboard Cite

Arsonium‐Containing Lipophosphoramides, Poly‐Functional Nano‐Carriers for Simultaneous Antibacterial Action and Eukaryotic Cell Transfection

Gall

Berchel

Hir

et al. 2013

Adv Healthcare Materials

View full text Add to dashboard Cite

Gene therapy of diseases like cystic fibrosis (CF) would consist of delivering a gene medicine towards the lungs via the respiratory tract into the target epithelial cells. Accordingly, poly-functional nano-carriers are required in order to overcome the various successive barriers of such a complex environment, such as airway colonization with bacterial strains. In this work, the antibacterial effectiveness of a series of cationic lipids is investigated before evaluating its compatibility with gene transfer into human bronchial epithelial cells. Among the various compounds considered, some bearing a trimethyl-arsonium headgroup demonstrate very potent biocide effects towards clinically relevant bacterial strains. In contrast to cationic lipids exhibiting no or insufficient antibacterial potency, arsonium-containing lipophosphoramides can simultaneously inhibit bacteria while delivering DNA into eukaryotic cells, as efficiently and safely as in absence of bacteria. Moreover, such vectors can demonstrate antibacterial activity in vitro while retaining high gene transfection efficiency to the nasal epithelium as well as to the lungs in mice in vivo. Arsonium-containing amphiphiles are the first synthetic compounds shown to achieve efficient gene delivery in the presence of bacteria, a property particularly suitable for gene therapy strategies under infected conditions such as within the airways of CF patients.

show abstract

Richness and diversity of mammalian fungal communities shape innate and adaptive immunity in health and disease

2014

View full text Add to dashboard Cite

Human holobiomes are networks of mutualistic interactions between human cells and complex communities of bacteria and fungi that colonize the human body. The immune system must tolerate colonization with commensal bacteria and fungi but defend against invasion by either organism. Molecular ecological surveys of the human prokaryotic microbiota performed to date have revealed a remarkable degree of bacterial diversity and functionality. However, there is a dearth of information regarding the eukaryotic composition of the microbiota. In this review, we describe the ecology and the human niches of our fungal "fellow travelers" in both health and disease, discriminating between passengers, colonizers, and pathogens based on the interaction of these fungi with the human immune system. We conclude by highlighting the need to reconsider the etiology of many fungal and immune-related diseases in the context of the crosstalk between the human system and its resident microbial communities.Keywords: Dysbiosis r Fungal metagenomics r Host-microbe interaction r Mycobiota IntroductionHumans live in close association with a complex community of bacteria, viruses, fungi, and archaea [1][2][3], which inhabit their bodies. Many groups have surveyed these microbial populations using the so-called "next generation" or "deep" sequencing approaches, revealing that the human microbiota differs radically at various body sites and among individuals [2][3][4]. The differences in the human microbiota are influenced by the availability of nutrients, environmental exposure to microorganisms, and other sitespecific features, such as the immunological makeup of a given location. The origin of differences in the microbiota between individuals potentially reflects different patterns of colonization early in life (reviewed in [5]), different dietary regimens [6,7], and different environmental exposures, such as antibiotic use [8,9]. Recently, the application of deep sequencing approaches have genCorrespondence: Dr. Duccio Cavalieri e-mail: duccio.cavalieri@fmach.it erated novel hypotheses about the microbial determinants underlying human disorders of immune origin (reviewed in [10]). Several metabolites of the interaction between diet and host microbiota, such as short-chain fatty acids, have been shown to play a fundamental role in shaping immune responses (reviewed in [11]). The application of microbial ecology concepts is ultimately leading to the conclusion that health and disease can be understood only through an understanding of the ways in which the symbiotic interactions between microbes and human organs harmonically integrate in the context of the hologenome [12].Human microbial diversity is not limited to bacteria; microorganisms such as fungi also play major roles in the stability of microbial communities in human health and disease (reviewed in [13]). Yeasts were detected in human stool samples as far back as 1917, and by the mid-20th century the presence of yeasts in the human intestine was proposed to have a saprotrophic role [14]....

show abstract

The Airway Microbiota in Cystic Fibrosis: A Complex Fungal and Bacterial Community—Implications for Therapeutic Management

Cited by 320 publications

References 103 publications

Un nouveau concept

Un nouveau concept

Arsonium‐Containing Lipophosphoramides, Poly‐Functional Nano‐Carriers for Simultaneous Antibacterial Action and Eukaryotic Cell Transfection

Richness and diversity of mammalian fungal communities shape innate and adaptive immunity in health and disease

Contact Info

Product

Resources

About