The adaptive response of Saccharomyces cerevisiae to mercury exposure

Westwater, John; McLaren, Niall F.; Dormer, Ulla H.; Jamieson, Derek J.

doi:10.1002/yea.835.abs

Cited by 14 publications

(18 citation statements)

References 26 publications

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“…4A), although the detection range for As was limited compared to that for Cd. Several studies reported that pathways for As and Hg detoxification in S. cerevisiae are similar to those for Cd detoxification (11,14,40,47), which is consistent with our observation that the ScSEO1 promoter is highly induced not only by Cd but also by As and Hg. The negligible response of the HpSEO1 promoter to Hg suggests that H. polymorpha has similar detoxification responses for As and Cd but uses a different defense mechanism against Hg toxicity.…”

Section: Discussionsupporting

confidence: 92%

Identification of the Cadmium-Inducible Hansenula polymorpha SEO1 Gene Promoter by Transcriptome Analysis and Its Application to Whole-Cell Heavy-Metal Detection Systems

Park

Sohn

et al. 2007

Appl Environ Microbiol

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The genomewide gene expression profiling of the methylotrophic yeast Hansenula polymorpha exposed to cadmium (Cd) allowed us to identify novel genes responsive to Cd treatment. To select genes whose promoters can be useful for construction of a cellular Cd biosensor, we further analyzed a set of H. polymorpha genes that exhibited >6-fold induction upon treatment with 300 M Cd for 2 h. The putative promoters, about 1,000-bp upstream fragments, of these genes were fused with the yeast-enhanced green fluorescence protein (GFP) gene. The resultant reporter cassettes were introduced into H. polymorpha to evaluate promoter strength and specificity. The promoter derived from the H. polymorpha SEO1 gene (HpSEO1) was shown to drive most strongly the expression of GFP upon Cd treatment among the tested promoters. The Cd-inducible activity was retained in the 500-bp deletion fragment of the HpSEO1 promoter but was abolished in the further truncated 250-bp fragment. The 500-bp HpSEO1 promoter directed specific expression of GFP upon exposure to Cd in a dose-dependent manner, with Cd detection ranging from 1 to 900 M. Comparative analysis of the Saccharomyces cerevisiae SEO1 (ScSEO1) promoter revealed that the ScSEO1 promoter has a broader specificity for heavy metals and is responsive to arsenic and mercury in addition to Cd. Our data demonstrate the potential use of the HpSEO1 promoter as a bioelement in whole-cell biosensors to monitor heavy metal contamination, particularly Cd.Industrial activities lead to large-scale environmental contamination with toxic heavy metals, such as cadmium (Cd) and mercury (Hg). These metals are toxic even at low levels and tend to accumulate in the body over an extended period of time, which can eventually cause serious health problems in humans. Therefore, the development of monitoring systems for these metal ions in the environment has become increasingly important in order to prevent chronic exposure to these pollutants (2). As a response to this need, numerous biological systems and nonbiological sensors based on the emerging nanotechnology have been developed to monitor heavy metal contamination (38, 43). In particular, biosensors that use unicellular microorganisms as analytical tools to monitor heavy metals have drawn attention because of several practical advantages (1): the large population size, rapid growth rate, low cost, and easy maintenance. Moreover, the feasibility of genetic manipulation makes microbial cells an attractive choice as environmental bioreporters.Many current microbial whole-cell sensors are based on genetically modified microorganisms (43,28). In general, microbial biosensors comprise the molecular fusion of two linked genetic elements: a sensing bioelement and a reporter gene. In most cases, the sensing element is a promoter that specifically responds to the presence or absence of the target molecule, and the reporter gene, which is fused to the sensing element, encodes a quantifiable molecule such as a bioluminescent or fluorescent protein (13). In creating ...

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Section: Discussionsupporting

confidence: 92%

Identification of the Cadmium-Inducible Hansenula polymorpha SEO1 Gene Promoter by Transcriptome Analysis and Its Application to Whole-Cell Heavy-Metal Detection Systems

Park

Sohn

et al. 2007

Appl Environ Microbiol

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“…For example, the oxidative stress produced by mercury exposure is similar to that produced by treatment with hydrogen peroxide, consistent with the finding that Yap1 is also involved in the response of yeast towards mercury (Westwater et al, 2002). Exposure to the excessive amounts of heavy metals 344 • Choi et al…”

Section: Discussionsupporting

confidence: 69%

“…Mitigation of endogenous mercury depends as a part on the presence of antioxidants such as GSH -most abundant intracellular nonprotein thiol that plays a central role in the maintenance of cellular redox status by quenching free radicals generated during oxidative stress (Jan et al, 2011). Expression of the gene encoding γ-glutamylcysteine synthetase is regulated by mercury and cadmium, but not other redox-active metals, including copper and iron, and its mercury-mediated regulation is not by the same mechanism used by cadmium (Westwater et al, 2002).…”

Section: 미생물학회지 제51권 제4호mentioning

confidence: 97%

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Participation of protein disulfide isomerase 2 in the tolerance against mercury toxicity in Schizosaccharomyces pombe

Choi¹,

Lim²,

Kim³

2015

The Korean Journal of Microbiology

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The present work was undertaken to address the role of protein disulfide isomerase 2 (Pdi2) in the mercury-tolerance of Schizosaccharomyces pombe, using the Pdi2-overexpressing recombinant plasmid pYPDI2 and the corresponding vector plasmid pRS316. When exposed to mercuric chloride, the PDI2 overepxression cells grew significantly better than the vector control cells. They revealed the lower levels of intracellular reactive oxygen species (ROS) and nitric oxide (NO), when incubated with mercuric chloride for 6 h, than the vector control cells. The PDI2 overepxression cells contained the higher levels of total glutathione (GSH) and superoxide dismutase (SOD) activity than the vector control cells, after 6 h of incubation in mercuric chloride. However, the PDI2 overepxression cells contained similar levels of glutathione peroxidase (GPx) activities, compared to those of the vector control cells. Taken together, the S. pombe Pdi2 promotes the tolerance against mercury toxicity through up-regulating total GSH and SOD and subsequently attenuating ROS and NO elevations.

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“…On the other hand, Yap1p and Skn7p regulators do not always act together and have some independent specialized functions [32,56,59]. Yap1p is also involved in heavy metal, diamide and diethylmaleate tolerance, whereas Skn7p is important for cell wall biosynthesis, cell cycle regulation and adaptation to osmotic stress [56,[60][61][62]. However, the functions of most genes regulated by Skn7p are still unknown [32,56].…”

Section: Regulation Of Global Responsementioning

confidence: 99%

Hydrogen peroxide-induced response in E. coli and S. cerevisiae: different stages of the flow of the genetic information

Semchyshyn

2009

Open Life Sciences

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Adaptation to oxidative stress is a major topic in basic and applied research. Cell response to stressful changes is realized through coordinated reorganization of gene expression. E. coli and S. cerevisiae are extremely amenable to genetic or molecular biological and biochemical approaches, which make these microorganisms suitable models to study stress response at a molecular level in prokaryotes and eukaryotes, respectively. The main focus of this review is (i) to discuss transcriptional control of global response to hydrogen peroxide in E. coli and S. cerevisiae, (ii) to summarize recent literature data on E. coli and S. cerevisiae adaptive response to oxidative stress at different stages of the flow of the genetic information: from transcription and translation to functionally active proteins and (iii) to discuss possible reasons for a lack of correlation between the expression of certain antioxidant genes at different levels of cellular organization.

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The adaptive response of Saccharomyces cerevisiae to mercury exposure

Cited by 14 publications

References 26 publications

Identification of the Cadmium-Inducible Hansenula polymorpha SEO1 Gene Promoter by Transcriptome Analysis and Its Application to Whole-Cell Heavy-Metal Detection Systems

Identification of the Cadmium-Inducible Hansenula polymorpha SEO1 Gene Promoter by Transcriptome Analysis and Its Application to Whole-Cell Heavy-Metal Detection Systems

Participation of protein disulfide isomerase 2 in the tolerance against mercury toxicity in Schizosaccharomyces pombe

Hydrogen peroxide-induced response in E. coli and S. cerevisiae: different stages of the flow of the genetic information

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