The actin-related protein Sac1 is required for morphogenesis and cell wall integrity in Candida albicans

Zhang, Bing; Yu, Qilin; Jia, Chang; Wang, Yuzhou; Xiao, Chenpeng; Dong, Yijie; Xu, Ning; Wang, Lei; Li, Mingchun

doi:10.1016/j.fgb.2014.12.007

Cited by 21 publications

(19 citation statements)

References 54 publications

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“…Recently, a Candida albicans homolog of Sac1 was identified . Although heterologous expression of C. albicans Sac1 rescued the inositol auxotrophic phenotype of S. cerevisiae sac1 mutants, deletion of sac1 in C. albicans did not cause inositol auxotrophy.…”

Section: Sac1 Function In Candida Albicansmentioning

confidence: 99%

“…Although actin stress fibers and MTs appear superficially normal, this phenotype suggests Sac1 may contribute to regulating cytoskeletal dynamics and cell migration. Candida albicans homolog of Sac1 was identified 168. Although heterologous expression of C. albicans Sac1 rescued the inositol auxotrophic phenotype of S. cerevisiae sac1 mutants, deletion of sac1 in C. albicans did not cause inositol auxotrophy.…”

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confidence: 99%

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Sac1, a lipid phosphatase at the interface of vesicular and nonvesicular transport

Bel

Brill

2018

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The lipid phosphatase Sac1 dephosphorylates phosphatidylinositol 4-phosphate (PI4P), thereby holding levels of this crucial membrane signaling molecule in check. Sac1 regulates multiple cellular processes, including cytoskeletal organization, membrane trafficking and cell signaling. Here, we review the structure and regulation of Sac1, its roles in cell signaling and development and its links to health and disease. Remarkably, many of the diverse roles attributed to Sac1 can be explained by the recent discovery of its requirement at membrane contact sites, where its consumption of PI4P is proposed to drive interorganelle transfer of other cellular lipids, thereby promoting normal lipid homeostasis within cells.

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Section: Sac1 Function In Candida Albicansmentioning

confidence: 99%

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confidence: 99%

Sac1, a lipid phosphatase at the interface of vesicular and nonvesicular transport

Bel

Brill

2018

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“…Proteomics analysis of biofilm matrix isolated using this optimized method revealed the presence of specific proteins (including glyceraldehyde 3-phosphate dehydrogenase and pyruvate kinase) in the biofilm matrix. Additional Candida genes implicated in biofilm formation include ACE2 ( 93 ), YWP1 ( 94 ), HWP1 ( 95 ), LL34 ( RIX7 ) ( 96 ), ALS3 ( 97 , 98 ), GAL10 ( 99 ), VPS1 ( 100 ), SUR7 ( 101 ), GUP1 ( 102 ), PEP12 ( 103 ), TPK1/2 ( 104 ), NRG1 (transcriptional repressor) and its target BRG1 (GATA family transcription factor) ( 105 ), UME6 (transcriptional regulator), HGC1 (a cyclin-related protein), SUN41 (a putative cell wall glycosidase), EFG1 ( 106 , 107 ), STV1 and VPH1 (Golgi/vacuolar subunits of vacuolar proton-translocating ATPase isoforms) ( 108 ), CEK1 (map kinase) ( 109 ), CDK8 ( 88 ), BCR1 ( 110 ), SPT20 ( 111 ), and SAC1 (PIP phosphatase) ( 112 ). In addition, quorum sensing molecules (such as 3R-hydroxy-tetradecaenoic acid [3R-HTDE, a beta-oxidation metabolite of endogenously present linoleic acid] [ 113 ]), farnesol ( 114 – 117 ), and cis -2-dodecenoic acid (BDSF) ( 118 ) and metabolic processes (e.g., carbohydrate assimilation, amino acid metabolism, and intracellular transport) ( 119 ) and glycolytic flux and hypoxia adaptation ( 120 ) have been suggested to play critical roles in Candida biofilm formation.…”

Section: Factors Influencing Biofilm Formation and Architecturementioning

confidence: 99%

Candida Biofilms: Development, Architecture, and Resistance

2015

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Intravascular device–related infections are often associated with biofilms (microbial communities encased within a polysaccharide-rich extracellular matrix) formed by pathogens on the surfaces of these devices. Candida species are the most common fungi isolated from catheter-, denture-, and voice prosthesis–associated infections and also are commonly isolated from contact lens–related infections (e.g., fungal keratitis). These biofilms exhibit decreased susceptibility to most antimicrobial agents, which contributes to the persistence of infection. Recent technological advances have facilitated the development of novel approaches to investigate the formation of biofilms and identify specific markers for biofilms. These studies have provided extensive knowledge of the effect of different variables, including growth time, nutrients, and physiological conditions, on biofilm formation, morphology, and architecture. In this article, we will focus on fungal biofilms (mainly Candida biofilms) and provide an update on the development, architecture, and resistance mechanisms of biofilms.

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“…In this case, Sac1 is thought to primarily dephosphorylate PI 4 P in the ER to create a gradient of PI 4 P at the ER-plasma membrane interface that drives the transport of phosphatidylserine to the plasma membrane (Maeda, et al ., 2013, Moser von Filseck, et al ., 2015). A sac1Δ mutant in C. albicans is very defective in cell wall integrity and virulence (Zhang, et al ., 2015). …”

Section: Barriers and Scaffolds In The Plasma Membrane: Mcc/eisosomesmentioning

confidence: 99%

Plasma membrane organization promotes virulence of the human fungal pathogen Candida albicans

Douglas

Konopka

2016

J Microbiol.

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Candida albicans is a human fungal pathogen capable of causing lethal systemic infections. The plasma membrane plays key roles in virulence because it not only functions as a protective barrier, it also mediates dynamic functions including secretion of virulence factors, cell wall synthesis, invasive hyphal morphogenesis, endocytosis, and nutrient uptake. Consistent with this functional complexity, the plasma membrane is composed of a wide array of lipids and proteins. These components are organized into distinct domains that will be the topic of this review. Some of the plasma membrane domains that will be described are known to act as scaffolds or barriers to diffusion, such as MCC/eisosomes, septins, and sites of contact with the endoplasmic reticulum. Other zones mediate dynamic processes, including secretion, endocytosis, and a special region at hyphal tips that facilitates rapid growth. The highly organized architecture of the plasma membrane facilitates the coordination of diverse functions and promotes the pathogenesis of C. albicans.

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The actin-related protein Sac1 is required for morphogenesis and cell wall integrity in Candida albicans

Cited by 21 publications

References 54 publications

Sac1, a lipid phosphatase at the interface of vesicular and nonvesicular transport

Sac1, a lipid phosphatase at the interface of vesicular and nonvesicular transport

Candida Biofilms: Development, Architecture, and Resistance

Plasma membrane organization promotes virulence of the human fungal pathogen Candida albicans

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