The absence of a functional nuclear receptor element A (NREA) in the promoter II of the aromatase P450 gene in rabbit granulosa cells

cAMP-dependent regulation of CYP19 gene in rabbit preovulatory granulosa cells and corpus luteum

“…In this recent study, we have shown that PII cAMP sensitivity in rabbit granulosa cells requires the involvement also the cAMP responsive element like sequence (CLS). Moreover, our data are in accordance with previous studies [4,5], which propose that additional ovarian-specific regulatory regions may also participate in this cAMP sensitivity [6].…”

“…In rabbit, the sequence of the NREA (nuclear receptor element A) site is not conserved, and this modification induces a decrease in affinity for the NR5A factors. So, this site seems to be non-functional in rabbits [6]. In this recent study, we have shown that PII cAMP sensitivity in rabbit granulosa cells requires the involvement also the cAMP responsive element like sequence (CLS).…”

Involvement of transcription factor GATA-4 in regulation of CYP19 gene during folliculogenesis and luteinization in buffalo ovary

“…The CBP in turn increases the histone acetyltransferase activity by recruiting HATs such as PCAF (CBP-associated factor) [17,54,55]. Furthermore, GATA-4 is predominantly phosphorylated on serine residue located at position 261 by PKA in response to cAMP in gonandal cells [19]. The above authors have also shown that PKA dependent phosphorylation of GATA-4 also led to enhanced recruitment of the CREB-binding protein (CBP) coactivator.…”

“…The regulatory sequence, cAMP-response element (CRE: 5 -TGACGTCA-3 ) that binds the cAMP-response element binding protein (CREB), has a single mutation in humans and rat and is designated as cAMP-response element-like sequence (CLS): 5 -TGcACGTCA-3 . This element is shown to be critical for cAMP stimulated transcription of human and rat ovarian CYP19 gene [19,35]. However, in cattle [22] and buffalo [7], the CYP19 gene has a single base pair deletion in a cAMP-response element-like sequence (CLS) and is designated as CLS-like sequence.…”

“…Phosphorylation of CREB Ser 133 leads to the recruitment of the co-activator CREB-binding protein (CBP), which associates with the transcriptional machinery leading to increased gene transcription [18]. In humans and rat, the consensus CRE has an insertion of single base pair and is designated as cAMP-responsive element like sequence (CLS) [19]. However, the sequence has also been shown to bind the phosphorylated CREB protein and is a functional ciselement [20,21].…”