The ability of maize Lancaster inbreds to callusogenesis in vitro under varying environmental conditions

Abstract

Aim. This work is focused on the estimation of the callusogenic potential of 10 maize Lancaster germplasm inbreds in comparison with well-known inbreds-standards with high callusogenic ability A188, Chi31 and PLS61, and the identification of genotypes with stable morphogenic callus formation under varying conditions of donor plant cultivation during three years of researches. Methods. Method of cell, tissue and organ culture in vitro. Field method. One-way and two-way analysis of variance. Results. For the investigated Lancaster inbreds the average multi-annual value of the total frequency of callusogenesis was 80.1 %, the frequency of morphogenic type I callus formation was 25.7 %, type II callus formation was 43.8 %, but for inbreds-standards these ones reached, respectively, 96.2 %, 12.2 % and 65.4 %. The level of callusogenesis varied depending on the year of investigations for all studied genotypes. The influence of genotype, year conditions and the combination of these factors on callus induction for most of the studied inbreds was significant. Conclusions. The impact of the interaction of a genotype and ecological factors of donor plant cultivation on morphogenic callusogenesis of type I as well as type II was the most significant forLancaster inbreds.Lancaster inbreds ДK298, ДK6080, ДK212 and ДK420-1 were the most stable under varying environmental conditions on the total frequency of callusogenesis, the frequencies of morphogenic and type II callus formation. None of the studied inbreds revealed the stability on type I callus formation frequency.
 Keywords: maize (Zea mays L.), Lancaster germplasm, callusogenesis, culture in vitro.

Origanum vulgare L. Cuttings Rhizogenesis in Microclonal Reproduction in Vitro

“…і лікарських [20], застосовується як для оптимізації біотехнологій мікроклонального розмноження цінних видів рослин, так і з використанням калюсних культур, культури пиляків тощо для біотехнологічного забезпечення селекційного процесу [21,22]. Утім дослідники наголошують на диференційованій генотиповій реакції за рахунок внутрішньовидової спадкової мінливості на варіювання як фітогормонального складу живильних середовищ, так і взагалі умов культивування [23,24].…”