PLoS ONE volume 9, issue 5, Pe98422 2014 DOI: 10.1371/journal.pone.0098422 View full text
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Wei-Yu Liao, Ting-Yung Ke, Hung-Yi Wu

Abstract: The synthesis of the negative-strand [(−)-strand] complement of the ∼30 kilobase, positive-strand [(+)-strand] coronaviral genome is a necessary early step for genome replication. The identification of cis-acting elements required for (−)-strand RNA synthesis in coronaviruses, however, has been hampered due to insufficiencies in the techniques used to detect the (−)-strand RNA species. Here, we employed a method of head-to-tail ligation and real-time quantitative reverse transcription polymerase chain reaction…

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