Thawing boar semen in the presence of seminal plasma: Effects on sperm quality and fertility

Garcia, José Carlos; Domínguez, Juan Carlos; Peña, Francisco; Alegre, B.; González, Rumualdo; Castro, M.J.; Habing, Gregory; Kirkwood, Roy N.

doi:10.1016/j.anireprosci.2009.11.001

Cited by 62 publications

(55 citation statements)

References 32 publications

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“…The different results in term of percentage of seminal plasma might be because Okazaki et al (2009) used a concentration of seminal plasma between 0 and 20% in post-thawing solutions. In agreement with our study, Garcia et al (2010) used concentration of seminal plasma of 0, 10, 50% (v/v) in post-thawing solutions and found that the latter concentration yielded a significantly higher percentage of post-thawing motility. Taking these results together, the percentage of seminal plasma in post-thawing solution is considered to be one of the factors for obtaining a betterquality of frozen-thawed boar semen.…”

Section: Discussionsupporting

confidence: 91%

A preliminary study on using autologous and heterologous boar sperm supernatant from freezing processes as post-thawing solution: its effect on sperm motility

Kaeoket

Chanapiwat

Tummaruk

et al. 2011

Trop Anim Health Prod

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The aim of this study was to evaluate the effect of post-thawing dilution with autologous and heterologous sperm supernatant on motility of frozen-thawed boar spermatozoa. During the cryopreservation, sperm supernatant (a combination of seminal plasma and semen extender, 50% v/v) or seminal plasma from nine boars (Duroc, Large White, and Landrace; three in each) was collected by centrifugation and stored frozen until use as post-thawing solution. Sperm pellets were further processed and cryopreserved using control-rate freezer and was thawed at 50°C for 12 s. After thawing, frozen thawed semen samples were diluted with seminal plasma (group A), supernatant from Landrace (group B), supernatant from Large White (group C), supernatant from Duroc (group D), and Modena™ semen extender (group E). Post-thawing motility was evaluated using a phase-contrast microscope after thawing at 1, 10, 20, and 30 min. The present results show that at 1 min, a significantly higher percentage (P ≤ 0.001) of progressive motility was found in groups B (53.3%) and C (53.9%) than the other groups. At 10 min, the highest (P ≤ 0.001) progressive motility was found in groups B (65%) and C (61%). At 20 and 30 min, a significantly higher percentage (P ≤ 0.001) of progressive motility was found in groups B (58.9%), C (53.5%), and D (45.6%) than groups A (3.9%) and E (20.6%). It can be stated that supernatant from the freezing processes (consisting of seminal plasma and Modena™, 50% v/v) had a beneficial effect on post-thawing progressive motility of frozen boar semen.

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Section: Discussionsupporting

confidence: 91%

A preliminary study on using autologous and heterologous boar sperm supernatant from freezing processes as post-thawing solution: its effect on sperm motility

Kaeoket

Chanapiwat

Tummaruk

et al. 2011

Trop Anim Health Prod

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“…For this reason, boar spermatozoa are commonly stored in liquid form at 15-18 °C for routine use in AI (Johnson et al, 2000;Purdy et al, 2010;Schmid et al, 2013;Schulze et al, 2013). In addition, swine AI involves the use of semen collected within the previous 3-7 days almost exclusively, although there is evidence to indicate that optimal fertility requires AI within 48 h after collection and extension in short-and long-term extenders (Johnson et al, 2000;Großfeld et al, 2008;Garcia et al, 2010). The components of the preservation solution and the temperature at which the semen is collected and stored following dilution are the main factors that influence the storage tolerance of spermatozoa preserved in a liquid state (Johnson et al, 2000;Martín-Hidalgo et al, 2013a;Schulze et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Storage temperature of boar semen and its relationship to changes in sperm plasma membrane integrity, mitochondrial membrane potential, and oxidoreductive capability

Gączarzewicz¹,

Udała²,

Piasecka³

et al. 2015

Turk J Biol

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The aim of this study was to analyze changes in sperm plasma membrane integrity, mitochondrial activity, and extracellular environment during storage of boar semen at 5 °C, 16 °C, and 25 °C for 10 days. Progressive sperm motility, plasma membrane integrity (SYBR-14/PI-test and HOS test), aspartate aminotransferase (AAT), and mitochondrial activity (JC-1-test and NADH-dependent NBT assay), as well as pH and osmolality were assessed in nine ejaculates of Polish Landrace boars. The plasma membrane integrity of the semen stored at 5 °C was similar to that of the semen stored at 16 °C and 25 °C; however, an increase in AAT activity of the semen stored at 5 °C revealed sperm membrane disorders as early as day 2. Significant differences in the progressive motility of the semen stored at 5 °C and 16 °C were observed at each of the evaluation times. This reduced motility was consistent with decreased sperm mitochondrial transmembrane potential and oxidoreductive capability. We inferred that the cooling of boar semen to 5 °C increases the permeability of the sperm plasma membrane, which may not be revealed by SYBR-14/PI staining or HOS testing. The loss of boar sperm motility that occurs during hypothermic liquid preservation may be connected with alterations in the plasma membrane stability and disorders of mitochondrial transmembrane potential and oxidoreductive capability.

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“…Namely, the sperm motility (57%), plasma membrane integrity (57%) and acrosome membrane integrity (57%) were significantly (P < 0.05) higher in samples with seminal plasma than in the control samples (51%, 50% and 49%, respectively). It has also been shown that the addition of seminal plasma to the media for thawing frozen boar semen is a key factor in achieving greater fertility in sows inseminated with frozenthawed AI doses (Okazaki et al 2009;Garcia et al 2010;Okazaki et al 2012). Flowers (1998) demonstrated that concentrations of seminal plasma proteins were highly correlated with in vitro boar semen fertility.…”

Section: Discussionmentioning

confidence: 99%

Motility of boar spermatozoa supplemented with homologous seminal plasma of high or low protein content after storage for three days

Apić¹,

Stančić²,

Vakanjac³

et al. 2016

Vet. Med. - Czech

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ABSTRACT:The effect of adding high or low protein content homologous seminal plasma to boar spermatozoa on the progressive motility of the spermatozoa after storage for three days, and at a 1 : 4 dilution ratio was investigated. A total of 32 ejaculates collected from four boars (eight ejaculates per boar) with high seminal plasma protein content (4%, HH-group) and 32 ejaculates collected from four boars (eight ejaculates per boar) with low seminal plasma protein content (2%, LL-group) were evaluated. The fresh ejaculate samples were centrifuged at 1000 g for 10 min at 4 °C to separate the spermatozoa from the seminal plasma. After centrifugation, one of the centrifuged spermatozoa samples was used to form an autologous fresh ejaculate sample, and another to form a homologous sample by adding low or high protein content seminal plasma from other boars. It was found that semen samples formed with spermatozoa from HH-group boars, supplemented with seminal plasma from LL-group, boars have significantly (P < 0.01) lower progressive motility (55%) after storage for 72 h than samples containing the boar's own (autologous) seminal plasma (65%). Conversely, when homologous seminal plasma with high protein content was added to the spermatozoa isolated from the boar ejaculate with low protein content in its seminal plasma, progressive motility significantly (P < 0.01) increased from 52% in samples with autologous seminal plasma, to 65% in samples with homologous seminal plasma. It was concluded that addition of homologous high protein content seminal plasma to the spermatozoa of boars with low protein content in their seminal plasma increases their progressive motility after storage for 72 h at a 1 : 4 dilution ratio. This could be a useful tool for increasing reproductive performance in lower fertility high genetic quality boars.

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Thawing boar semen in the presence of seminal plasma: Effects on sperm quality and fertility

Cited by 62 publications

References 32 publications

A preliminary study on using autologous and heterologous boar sperm supernatant from freezing processes as post-thawing solution: its effect on sperm motility

A preliminary study on using autologous and heterologous boar sperm supernatant from freezing processes as post-thawing solution: its effect on sperm motility

Storage temperature of boar semen and its relationship to changes in sperm plasma membrane integrity, mitochondrial membrane potential, and oxidoreductive capability

Motility of boar spermatozoa supplemented with homologous seminal plasma of high or low protein content after storage for three days

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