\(\textit{In vitro}\) antioxidant, α-amylase and α-glucosidase inhibitory activities of endophytic bacteria from the roots of the mangrove plant \(\textit{Rhizophora stylosa}\) Griffith

Abstract

Mangrove is one of the highly productive ecosystems and contains diverse plants and microbial communities. Bacterial endophytes from mangroves are considered as a prolific source of biological molecules with important functions in the protection of mangrove plants against herbivores, insects as well as pathogens. The present study aimed to isolate endophytic bacteria from the roots of mangrove plant Rhizophora stylosa and to screen antioxidant,

Pharmacological Properties, Volatile Organic Compounds, and Genome Sequences of Bacterial Endophytes from the Mangrove Plant Rhizophora apiculata Blume

“…In the present study, twenty-three endophytic bacteria belonging to nine genera (i.e., Microbulbifer , Streptomyces , Bacillus , Rossellomorea , Micrococcus , Paracoccus , Microbacterium , Pseudovibrio , and Brevibacterium ) were isolated from the fresh roots of R. apiculata . Previous investigations revealed that the genera Bacillus , Streptomyces , and Pseudovibrio have been isolated frequently from mangrove plants, whereas Microbulbifer , Micrococcus , Paracoccus , Microbacterium , and Brevibacterium have been isolated less frequently [ 10 , 70 , 71 , 72 , 73 ]. Genome analyses of the genera Bacillus , Streptomyces , and Pseudovibrio have reported that genomes of these genera contain versatile and diverse genes of metabolic pathways as well as genes linked to symbiosis and lifestyles, allowing for host switching and adapting to various environments [ 74 , 75 , 76 , 77 , 78 , 79 , 80 ].…”

“…The plates were incubated at 30 °C for 3–5 days. After incubation, colonies with different morphological characteristics (e.g., color, shape, and consistency) were streaked on nutrient agar plates (NA, Himedia, Mumbai, India) to obtain the pure isolates [ 70 ].…”

“…The antioxidant effect of the extracts was determined by DPPH and ABTS radical scavenging assays [ 70 ].…”

“…The α-amylase (A8220, Sigma-Aldrich, St. Louis, MO, USA) enzyme inhibitory effect of the extracts was determined according to the described method by Dat et al [ 70 ]. In brief, starch azure was suspended in 0.05 M Tris-HCl buffer (pH 6.9) containing 0.01 M CaCl 2 , and the substrate solution was then boiled for 5 min and pre-incubated at 37 °C for 5 min.…”

Metabolites extracted from microorganisms as potential inhibitors of glycosidases (α-glucosidase and α-amylase): A review

“…They were identified as Bacillus , Streptomyces and Pseudomvibrio . The ethyl acetate extracts of five isolated strains showed inhibitory activities against α-amylase with values ranging from 31.4 ± 3.1% to 59.7 ± 6.4% ( Dat and Thuy, 2021 ). Further, Dat et al (2022) investigated an endophytic Bacillus sp.…”