“…15 Nuclear and cytosolic proteins were extracted with NucBuster protein extraction kit (Novagen, Madison, WI, USA) according to manufacturer's instructions. Rabbit anti-human antibodies were used for detection of the following: pmTOR(Ser2448)/total mTOR, peIF4E(Ser209)/total eIF4E, pAkt(Ser473)/total Akt, pSTAT3 (Ser727/Tyr705)/total STAT3, pERK1/2(Thr202/Tyr204)/ total ERK1/2, pMNK(Thr 197/202)/total MNK, p4EBP (Ser65)/total 4EBP, Cyclin D, c-Myc (Cell Signaling Technology), HSC-70, total VEGFR1 (Santa Cruz, CA, USA), pVEGFR1(Tyr 1213; R&D Systems, Minneapolis, MN, USA) and LC3/LC3II (Sigma).…”