Tetrahedral framework nucleic acids promote scarless healing of cutaneous wounds via the AKT-signaling pathway

Zhu, Junyao; Zhang, Mei; Gao, Yang; Qin, Xin; Zhang, Tianxu; Cui, Weitong; Shen, Xian; Xiao, Dexuan; Lin, Yunfeng

doi:10.1038/s41392-020-0173-3

Cited by 50 publications

(58 citation statements)

References 46 publications

(54 reference statements)

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“…Polyacrylamide gel electrophoresis (PAGE) and highperformance capillary electrophoresis were performed as previously described [27][28][29]. The UV absorbances of RSV, tFNAs, and tFNAs-RSV were detected using an ultramicrospectrophotometer.…”

Section: Characterization Of Tfnas-rsvmentioning

confidence: 99%

Tetrahedral Framework Nucleic Acid-Based Delivery of Resveratrol Alleviates Insulin Resistance: From Innate to Adaptive Immunity

Gao

Shi

et al. 2021

Nano-Micro Lett.Has correction 2021-9-3 Has erratum 2021-1

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Obesity-induced insulin resistance is the hallmark of metabolic syndrome, and chronic, low-grade tissue inflammation links obesity to insulin resistance through the activation of tissue-infiltrating immune cells. Current therapeutic approaches lack efficacy and immunomodulatory capacity. Thus, a new therapeutic approach is needed to prevent chronic inflammation and alleviate insulin resistance. Here, we synthesized a tetrahedral framework nucleic acid (tFNA) nanoparticle that carried resveratrol (RSV) to inhibit tissue inflammation and improve insulin sensitivity in obese mice. The prepared nanoparticles, namely tFNAs-RSV, possessed the characteristics of simple synthesis, stable properties, good water solubility, and superior biocompatibility. The tFNA-based delivery ameliorated the lability of RSV and enhanced its therapeutic efficacy. In high-fat diet (HFD)-fed mice, the administration of tFNAs-RSV ameliorated insulin resistance by alleviating inflammation status. tFNAs-RSV could reverse M1 phenotype macrophages in tissues to M2 phenotype macrophages. As for adaptive immunity, the prepared nanoparticles could repress the activation of Th1 and Th17 and promote Th2 and Treg, leading to the alleviation of insulin resistance. Furthermore, this study is the first to demonstrate that tFNAs, a nucleic acid material, possess immunomodulatory capacity. Collectively, our findings demonstrate that tFNAs-RSV alleviate insulin resistance and ameliorate inflammation in HFD mice, suggesting that nucleic acid materials or nucleic acid-based delivery systems may be a potential agent for the treatment of insulin resistance and obesity-related metabolic diseases.

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Section: Characterization Of Tfnas-rsvmentioning

confidence: 99%

Tetrahedral Framework Nucleic Acid-Based Delivery of Resveratrol Alleviates Insulin Resistance: From Innate to Adaptive Immunity

Gao

Shi

et al. 2021

Nano-Micro Lett.Has correction 2021-9-3 Has erratum 2021-1

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“…Bacterial nucleic acids can be used to detect the pathogenicity and existence of drug-resistant genes in microorganisms [ 9 , 10 ]. Four single-stranded DNA (ssDNA) molecules self-assemble into tetrahedral configurations, forming Tetrahedral Framework Nucleic Acids (tFNAs), based on the highly specific complementary base pairing principle [ [11] , [12] , [13] ]. Previous studies showed that tFNAs have an excellent biocompatibility and a favorable ability to permeate bacterial and mammalian cells, that have been recognized as a promising delivery vehicle used in delivering drug molecules [ [14] , [15] , [16] , [17] , [18] , [19] ].…”

Section: Introductionmentioning

confidence: 99%

Erythromycin loaded by tetrahedral framework nucleic acids are more antimicrobial sensitive against Escherichia coli (E. coli)

Sun

Liu

Zhang

et al. 2021

Bioactive Materials

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Erythromycin is a commonly used broad-spectrum antibiotic, but resistance to this antibiotic makes its use less effective. Considerable efforts, beside finding alternatives, are needed to enhance its antimicrobial effect and stability against bacteria. Tetrahedral framework nucleic acids (tFNAs), a novel delivery vehicle with a three-dimensional nanostructure, have been studied as a carrying platform of antineoplastic drugs. In this study, the use of tFNAs in delivering erythromycin into Escherichia coli ( E. coli ) was investigated for the first time. The tFNAs vehicle increased the bacterial uptake of erythromycin and promoted membrane destabilization. Moreover, it increased the permeability of the bacterial cell wall, and reduced drug resistance by improving the movement of the drug across the membrane. The tFNAs-based delivery system enhanced the effects of erythromycin against E. coli . It may therefore provide an effective delivery vehicle for erythromycin in targeting antibiotic-resistant bacteria with thick cell wall.

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“…Conventionally, TDNs comprise four different single‐stranded DNA (ssDNA) and self‐assemble according to the specific sequence of ssDNA based on complementary pairing rules (Figure 1A). 25 Eight percent PAGE was performed to evaluate the synthesis and characterization of TDNs. Figure 1B showed the successful synthesis of TDNs ranging around 200 bp in size.…”

Section: Resultsmentioning

confidence: 99%

“…Recently, tetrahedral DNA nanostructures (TDNs) have attracted increasing interest because of their editability and biocompatibility 22‐25 . Despite the polyanionic nature of DNA, TDNs can be internalized via endocytosis and transported into lysosomes in a microtubule‐dependent manner 26,27 .…”

Section: Introductionmentioning

confidence: 99%

Tetrahedral DNA nanostructure improves transport efficiency and anti‐fungal effect of histatin 5 against Candida albicans

et al. 2021

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Objectives Anti‐microbial peptides (AMPs) have been comprehensively investigated as a novel alternative to traditional antibiotics against microorganisms. Meanwhile, Tetrahedral DNA nanostructures (TDNs) have gained attention in the field of biomedicine for their premium biological effects and transportation efficiency as delivery vehicles. Hence, in this study, TDN/Histatin 5 (His‐5) was synthesized and the transport efficiency and anti‐fungal effect were measured to evaluate the promotion of His‐5 modified by TDNs. Materials and Methods Tetrahedral DNA nanostructures/His‐5 complex was prepared via electrostatic attraction and characterized by transmission electron microscopy (TEM), polyacrylamide gel electrophoresis (PAGE), dynamic light scattering (DLS) and electrophoretic light scattering (ELS). The anti‐fungal effect of the TDN/His‐5 complex was evaluated by determining the growth curve and colony‐forming units of C. albicans. The morphological transformation of C. albicans was observed by light microscope and scanning electron microscope (SEM). Immunofluorescence was performed, and potassium efflux was detected to mechanistically demonstrate the efficacy of TDN/His‐5. Results The results showed that Histatin 5 modified by TDNs had preferable stability in serum and was effectively transported into C. albicans, leading to the increased formation of intracellular reactive oxygen species, higher potassium efflux and enhanced anti‐fungal effect against C. albicans. Conclusions Our study showed that TDN/His‐5 was synthesized successfully. And by the modification of TDNs, His‐5 showed increased transport efficiency and improved anti‐fungal effect.

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Tetrahedral framework nucleic acids promote scarless healing of cutaneous wounds via the AKT-signaling pathway

Cited by 50 publications

References 46 publications

Tetrahedral Framework Nucleic Acid-Based Delivery of Resveratrol Alleviates Insulin Resistance: From Innate to Adaptive Immunity

Tetrahedral Framework Nucleic Acid-Based Delivery of Resveratrol Alleviates Insulin Resistance: From Innate to Adaptive Immunity

Erythromycin loaded by tetrahedral framework nucleic acids are more antimicrobial sensitive against Escherichia coli (E. coli)

Tetrahedral DNA nanostructure improves transport efficiency and anti‐fungal effect of histatin 5 against Candida albicans

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