2019
DOI: 10.1002/smll.201901907
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Tetrahedral Framework Nucleic Acids Promote Corneal Epithelial Wound Healing in Vitro and in Vivo

Abstract: Poor post‐traumatic wound healing can affect the normal function of damaged tissues and organs. For example, poor healing of corneal epithelial injuries may lead to permanent visual impairment. It is of great importance to find a therapeutic way to promote wound closure. Tetrahedral framework nucleic acids (tFNAs) are new promising nanomaterials, which can affect the biological behavior of cells. In the experiment, corneal wound healing is used as an example to explore the effect of tFNAs on wound healing. Res… Show more

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Cited by 57 publications
(52 citation statements)
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“…Previous studies have indicated that tFNAs can promote the proliferation of various cell types. 24 27 , 29 , 34 , 35 Therefore, in this research, we aimed to determine whether this type of effect also existed in HaCaT and HSF cells. CCK-8 assays were used to examine cell proliferation, while cell-cycle assays were used to analyze changes in the number of cells at each stage.…”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…Previous studies have indicated that tFNAs can promote the proliferation of various cell types. 24 27 , 29 , 34 , 35 Therefore, in this research, we aimed to determine whether this type of effect also existed in HaCaT and HSF cells. CCK-8 assays were used to examine cell proliferation, while cell-cycle assays were used to analyze changes in the number of cells at each stage.…”
Section: Discussionmentioning
confidence: 99%
“…As shown in previous studies, tFNAs are able to promote cell migration. 27 29 , 35 Scratch experiments are widely performed to investigate cell migration in vitro, but there are still factors that can interfere with these outcomes. In this study, to minimize variability, all scratches were made by the same person.…”
Section: Discussionmentioning
confidence: 99%
“…Total protein was isolated from cells using a total protein extraction kit (Keygen Biotech, Nanjing, China). The protein samples were mixed with loading buffer, boiled for 5 min, separated by SDS-PAGE, and transferred to polyvinylidene fluoride membranes [29,30]. The membranes were blocked with 5% dry skim milk in Tris-buffered saline with 0.05% (v/v) Tween-20 (TBST) for 1 h and then incubated with primary antibodies against JKAMP (NBP2-36446SS) (Novus, Littleton, USA), GAPDH (ab181602), JNK1 (ab110724), β-Catenin (ab32572), RUNX2 (ab92336) and OPN (ab8448) (Abcam, Cambridge, UK), GSK-3β (12456), or p-GSK-3β (5558) (Cell Signaling Technology, Danvers, USA) at 4°C overnight.…”
Section: Western Blot Analysismentioning
confidence: 99%
“…Total protein was isolated from cells using a total protein extraction kit (Keygen Biotech, Nanjing, China). The protein samples were mixed with loading buffer, boiled for 5 minutes, separated by SDS-PAGE, and transferred to polyvinylidene fluoride membranes [27,28]. The membranes were blocked with 5% dry skim milk in Tris-buffered saline with 0.05% Figure 1B, C).…”
Section: Western Blot Analysismentioning
confidence: 99%