Tetragonal Chicken Egg White Lysozyme Solubility in Sodium Chloride Solutions

Abstract

The solubility of chicken egg white lysozyme crystallized in the tetragonal form was measured in sodium chloride solutions from 1.6 to 30.7 °C, using a miniature column solubility apparatus. Sodium chloride solution concentrations ranged from 1 to 7% (w/v). The solutions were buffered with 0.1 M sodium acetate buffer, with the solubility being measured at pH values in 0.2 pH unit increments in the pH range 4.0−5.4, with data also included at pH 4.5. Lysozyme solubility was found to increase with increasing in temperature and decreasing salt concentration. Solution pH has a varied and unpredictable effect on solubility.

Protein Crystallization As a Process Step in a Novel Meso Oscillatory Flow Reactor: Study of Lysozyme Phase Behavior

“…The solubility 39,40 and the metastability 41 curves were drawn based on the data available in the literature at quiescent conditions. Clear solutions above the solubility curve were indicative of the metastable region, once in spite of being supersaturated these solutions…”

“…Based on lysozyme phase behavior (Figure 4 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 and metastability curves reported in the literature for microbatch experiments 39,40 were linearized (see Figure 6) according to Martins et al 40 equation:…”

Probabilistic approach to lysozyme crystal nucleation kinetics

“…Chicken egg white lysozyme (Sigma, 3x crystallized) was used at six different concentrations, defining a particular supersaturation under the following other conditions: sodium chloride 5% (w/v), 0.1 M sodium acetate buffer, pH 4.0, temperature of 22°C. At these conditions the lysozyme solubility is reported to be 2.12 mg/ml, determined with an accuracy of ±0.1 mg/ml or better [9]. The experimental setup contains the following elements:…”

Reaching One Single and Stable Critical Cluster through Finite-Sized Systems

“…The solution, a small droplet, is the following: water, pH 4.5, NaCl (4% w/v), NaAc (0.1 M) (for lysozyme solubility see). 15 Results are calculated for the temperature T ) 293.15 K, so that solubility is equal to 3.1 mg/mL. The interfacial energy γ is here taken to be γ ) 1 mJ m -2 .…”